Lactoferrin's anti-cancer properties. Safety, selectivity, and wide range of action

Despite recent advances in cancer therapy, current treatments, including radiotherapy, chemotherapy, and immunotherapy, although beneficial, present attendant side effects and long-term sequelae, usually more or less affecting quality of life of the patients. Indeed, except for most of the immunotherapeutic agents, the complete lack of selectivity between normal and cancer cells for radio- and chemotherapy can make them potential antagonists of the host anti-cancer self-defense over time. Recently, the use of nutraceuticals as natural compounds corroborating anti-cancer standard therapy is emerging as a promising tool for their relative abundance, bioavailability, safety, low-cost effectiveness, and immuno-compatibility with the host. In this review, we outlined the anti-cancer properties of Lactoferrin (Lf), an iron-binding glycoprotein of the innate immune defense. Lf shows high bioavailability after oral administration, high selectivity toward cancer cells, and a wide range of molecular targets controlling tumor proliferation, survival, migration, invasion, and metastasization. Of note, Lf is able to promote or inhibit cell proliferation and migration depending on whether it acts upon normal or cancerous cells, respectively. Importantly, Lf administration is highly tolerated and does not present significant adverse effects. Moreover, Lf can prevent development or inhibit cancer growth by boosting adaptive immune response. Finally, Lf was recently found to be an ideal carrier for chemotherapeutics, even for the treatment of brain tumors due to its ability to cross the blood-brain barrier, thus globally appearing as a promising tool for cancer prevention and treatment, especially in combination therapies

Automated Meteor Fluxes with a Wide-Field Meteor Camera Network

Within NASA, the Meteoroid Environment Office (MEO) is charged to monitor the meteoroid environment in near ]earth space for the protection of satellites and spacecraft. The MEO has recently established a two ]station system to calculate automated meteor fluxes in the millimeter ]size ]range. The cameras each consist of a 17 mm focal length Schneider lens on a Watec 902H2 Ultimate CCD video camera, producing a 21.7 x 16.3 degree field of view. This configuration has a red ]sensitive limiting meteor magnitude of about +5. The stations are located in the South Eastern USA, 31.8 kilometers apart, and are aimed at a location 90 km above a point 50 km equidistant from each station, which optimizes the common volume. Both single station and double station fluxes are found, each having benefits; more meteors will be detected in a single camera than will be seen in both cameras, producing a better determined flux, but double station detections allow for non ]ambiguous shower associations and permit speed/orbit determinations. Video from the cameras are fed into Linux computers running the ASGARD (All Sky and Guided Automatic Real ]time Detection) software, created by Rob Weryk of the University of Western Ontario Meteor Physics Group. ASGARD performs the meteor detection/photometry, and invokes the MILIG and MORB codes to determine the trajectory, speed, and orbit of the meteor. A subroutine in ASGARD allows for the approximate shower identification in single station meteors. The ASGARD output is used in routines to calculate the flux in units of #/sq km/hour. The flux algorithm employed here differs from others currently in use in that it does not assume a single height for all meteors observed in the common camera volume. In the MEO system, the volume is broken up into a set of height intervals, with the collecting areas determined by the radiant of active shower or sporadic source. The flux per height interval is summed to obtain the total meteor flux. As ASGARD also computes the meteor mass from the photometry, a mass flux can be also calculated. Weather conditions in the southeastern United States are seldom ideal, which introduces the difficulty of a variable sky background. First a weather algorithm indicates if sky conditions are clear enough to calculate fluxes, at which point a limiting magnitude algorithm is employed. The limiting magnitude algorithm performs a fit of stellar magnitudes vs camera intensities. The stellar limiting magnitude is derived from this and easily converted to a limiting meteor magnitude for the active shower or sporadic source

Video gaming in a hyperconnected world: A cross-sectional study of heavy gaming, problematic gaming symptoms, and online socializing in adolescents

Aims Examining online social interactions along with patterns of video gaming behaviors and game addiction symptoms has the potential to enrich our understanding of disorders related to excessive video game play. Methods We performed latent class analysis in a sample of 9733 adolescents based on heavy use of games, social networking and instant messaging, and game addiction symptoms. We used latent class regression to determine associations between classes, psychosocial well-being and friendship quality. Results We identified two types of heavy gaming classes that differed in probability of online social interaction. Classes with more online social interaction reported fewer problematic gaming symptoms than those with less online social interaction. Most adolescents estimated to be in heavy gaming classes had more depressive symptoms than normative classes. Male non-social gamers had more social anxiety. Female social gamers had less social anxiety and loneliness, but lower self-esteem. Friendship quality attenuated depression in some male social gamers, but strengthened associations with loneliness in some male non-social gamers. Conclusions In adolescents, symptoms of video game addiction depend not only on video game play but also on concurrent levels of online communication, and those who are very socially active online report fewer symptoms of game addiction

REGULATION OF PROSTAGLANDIN GENERATION IN CARRAGEENAN-INDUCED PLEURISY BY INDUCIBLE NITRIC OXIDE SYNTHASE IN KNOCKOUT MICE

In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p < 0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p < 0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p < 0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis