An Ergonomic Smart Eye Designed for Reading and Object Detection for Blind People using IOT

The ability to see is a wonderful blessing.Vision helps people to see and understand their surroundings.Blind people have trouble reading books and detecting  objects in front of them reading books and detecting  objects in front of them. This research paper  focuses on the development of an artificial eye that uses a web camera to enhance text reading and for object recognition. A system with additional functionality is designed to support blind people.It is a visual design composed of a few key components such as a camera, Raspberry Pi, and earphones installed together, as well as additional web-based operating technology intertwined. The image collected and processed with the aid of the camera interfaced to the Raspberry pi/IOT technology is the project's input. As a result, the text and objects are detected, and audio information is sent to the blind man through earphones. The objective of this research is to make a low-cost, easy-to-use portable device that can be used anywhere while walking avoiding hurdles and that can also facilitate blind people in reading articles, books, and gaining knowledge

Androgen-responsive non-coding small RNAs extend the potential of HCG stimulation to act as a bioassay of androgen sufficiency

Background: It is unclear whether a short-term change in circulating androgens is associated with changes in the transcriptome of the peripheral blood mononuclear cells (PBMC). Aims & Methods: To explore the effect of hCG-stimulation on the PBMC-transcriptome, 12 boys with a median age (range) of 0.7yrs (0.3, 11.2) who received intramuscular hCG 1500u on 3 consecutive days as part of their investigations underwent transcriptomic array analysis on RNA extracted from peripheral blood mononuclear cells before and after hCG stimulation. Results: Median pre and post hCG testosterone for the overall group was 0.7nmol/l (<0.5,6) and 7.9nmol/l (<0.5, 31.5), respectively. Of the 12 boys, 3 (25%) did not respond to hCG stimulation with a pre and post median serum testosterone of <0.5nmol/l and <0.5nmol/l, respectively. When corrected for gene expression changes in the non-responders to exclude hCG effects, all 9 of the hCG responders consistently demonstrated a 20% or greater increase in the expression of piR-37153 and piR-39248, non-coding PIWI-interacting RNAs (piRNAs). In addition, of the 9 responders, 8, 6 and 4 demonstrated a 30%, 40% and 50% rise, respectively in a total of 2 further piRNAs. In addition, 3 of the responders showed a 50% or greater rise in the expression of another small RNA, SNORD5. On comparing fold change in serum testosterone with fold change in the above transcripts, a positive correlation was detected for SNORD5 (p=0.01). Conclusions: The identification of a dynamic and androgen-responsive PBMC-transcriptome extends the potential value of the hCG test for assessment of androgen sufficiency

Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ES cell differentiation without affecting naive to primed cell transition

The differentiation of mouse embryonic stem (ES) cells is controlled by the interaction of multiple signaling pathways, typically mediated by post-translational protein modifications. The addition of O-linked N-acetylglucosamine (O-GlcNAc) to serine and threonine residues of nuclear and cytoplasmic proteins is one such modification (O-GlcNAcylation), whose function in ES cells is only now beginning to be elucidated. Here we demonstrate that the specific inhibition of O-GlcNAc hydrolase (Oga) causes increased levels of protein O-GlcNAcylation and impairs differentiation of mouse ES cells both in serum-free monolayer and in embryoid bodies (EBs). Use of reporter cell lines demonstrates that Oga inhibition leads to a reduction in the number of Sox1-expressing neural progenitors generated following induction of neural differentiation, as well as maintained expression of the ES cell marker Oct4 (Pou5f1). In EBs expression of mesodermal and endodermal markers is also delayed. However, the transition of naïve cells to primed pluripotency indicated by Rex1 (Zfp42), Nanog, Esrrb and Dppa3 downregulation and Fgf5 upregulation remains unchanged. Finally, we demonstrate that increased O-GlcNAcylation results in upregulation of genes normally epigenetically silenced in ES cells, supporting the emerging role for this protein modification in the regulation of histone modifications and DNA methylation. Stem Cells 2014

Quantitative proteomics in resected renal cancer tissue for biomarker discovery and profiling

<b>Background:</b>  Proteomics-based approaches for biomarker discovery are promising strategies used in cancer research. We present state-of-art label-free quantitative proteomics method to assess proteome of renal cell carcinoma (RCC) compared with noncancer renal tissues.<p></p> <b>Methods:</b>  Fresh frozen tissue samples from eight primary RCC lesions and autologous adjacent normal renal tissues were obtained from surgically resected tumour-bearing kidneys. Proteins were extracted by complete solubilisation of tissues using filter-aided sample preparation (FASP) method. Trypsin digested proteins were analysed using quantitative label-free proteomics approach followed by data interpretation and pathways analysis.<p></p> <b>Results:</b>  A total of 1761 proteins were identified and quantified with high confidence (MASCOT ion score threshold of 35 and P-value <0.05). Of these, 596 proteins were identified as differentially expressed between cancer and noncancer tissues. Two upregulated proteins in tumour samples (adipose differentiation-related protein and Coronin 1A) were further validated by immunohistochemistry. Pathway analysis using IPA, KOBAS 2.0, DAVID functional annotation and FLink tools showed enrichment of many cancer-related biological processes and pathways such as oxidative phosphorylation, glycolysis and amino acid synthetic pathways.<p></p> <b>Conclusions:<b>  Our study identified a number of differentially expressed proteins and pathways using label-free proteomics approach in RCC compared with normal tissue samples. Two proteins validated in this study are the focus of on-going research in a large cohort of patients.<p></p&gt

Kinetics and spectral properties of electron and <SUP>&#8226;</SUP>OH adducts of dimethylpyridines: a pulse radiolysis study

The reactions of e-aq, &#8226;OH&#8226;-, O&#8226;- and SO&#8226;-4 with 2,4-, 2,6- and 3,5-dimethylpyridines have been investigated in aqueous solution by pulse radiolysis with optical detection. Both e-aq and &#8226;OH radicals have high reactivity toward these compounds with k = 4-8&#215;109 dm3 mol-1 s-1. The rates of O&#8226; and SO&#8226;-4 reactions (1-3 &#215; 109 dm3 mol-1 s-1 were lower compared to the rate observed with the &#8226;OH radical. The transient absorption spectra obtained in the reaction of e-aq with three isomers exhibited a weak broad band around 340-410 nm. The absorption maxima of the intermediates formed in the &#8226;OH and SO&#8226;-4 reactions were centred around 320-330 nm (&#949;= 2450- 3500 dm3 mol-1 cm-1/ with an additional broad peak in the range 460-520 nm which are attributed to the corresponding &#8226;OH adducts. The spectra in the O&#8226;- reaction have absorption maxima between 300 and 320 nm and it reacts both by addition and H-abstraction from the CH3 group. A reaction mechanism consistent with the observed results is proposed

Design of a Fractional Order PI (FOPI) for the speed control of a high-performance electrical drive with induction motor

This paper describes the application of the Fractional Order PIs (FOPI) in the speed loop of a high performance induction motor electrical drive. In particular the speed tracking and load rejection capability of FOPI controller has been investigated and compared with both an integer-order PI and an IP both in simulation and experimentally with constant settling time. Illustrative study proves the simplicity and efficiency of the presented design method over integer controllers

Promising Practices From Fiji in Empowering Women Economically: Learnings From Talanoa Treks, Ra Naari Parishad, Rise Beyond the Reef, and the Fiji Womens Fund

This paper is jointly authored by eight women who work with the Fiji Women's Fund and three of the Fund's partner organisations - Talanoa Treks, Ra Naari Parishad, and, Rise Beyond the Reef. The paper aims to contribute to improved women's economic empowerment programs by sharing the experiences of these three partners. The authors document the learnings of practitioners in Fiji and compare these with the existing literature for the audience of practitioners in the Pacific and abroad. The Fiji Women's Fund supports the documentation of research from practice, so that the expertise of practitioners is recognised, and, to increase the body of knowledge generated from the Global South.The paper examines the experiences and learnings of the three partners using the Gender at Work framework, developed by Rao and Kelleher, which highlights the inter-linked dimensions of change required to achieve sustainable progress on gender equality and women's empowerment. The paper documents the similar journey taken by all three partner organisations, through each of the four quadrants of this framework. All three entities supported the establishment of a formal, collective structure being established, to provide women access to training and income-generating opportunities. Women accessed these opportunities to improve their skills, capabilities, income and assets. These changes, in turn, had an influence on the way the women themselves, and the men in their lives, think about what it means to be a woman or a man and the possibilities available. For example, there is evidence of positive changes to what women and men are doing in their households. Husbands, sons and partners are helping women beneficiaries by taking on some of the care tasks that were previously left to the women. The greatest evidence of change is within households, as changes to exclusionary practices at the village level are less evident

Detection of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients

BACKGROUND: Diagnosis of tuberculous meningitis (TBM) is difficult. Rapid confirmatory diagnosis is essential to initiate required therapy. There are very few published reports about the diagnostic significance of 65 kD heat shock protein (hsp) in TBM patients, which is present in a wide range of Mycobacterium tuberculosis species and elicits a cellular and humoral immune response. In the present study we have conducted a prospective evaluation for the demonstration of 65 kD hsp antigen in cerebrospinal fluid (CSF) of TBM patients, by indirect ELISA method using monoclonal antibodies (mAb) against the 65 kD hsp antigen, for the diagnosis of TBM. METHODS: A total of 160 CSF samples of different groups of patients (confirmed TBM {n = 18}, clinically suspected TBM {n = 62}, non TBM infectious meningitis {n = 35} and non-infectious neurological diseases {n = 45}) were analyzed by indirect ELISA method using mAb to 65 kD hsp antigen. The Kruskal Wallis test (Non-Parametric ANOVA) with the Dunnett post test was used for statistical analysis. RESULTS: The indirect ELISA method yielded 84% sensitivity and 90% specificity for the diagnosis of TBM using mAb to 65 kD hsp antigen. The mean absorbance value of 65 kD hsp antigen in TBM patients was [0.70 ± 0.23 (0.23–1.29)], significantly higher than the non-TBM infectious meningitis group [0.32 ± 0.14 (0.12–0.78), P < 0.001] and also higher than the non-infectious neurological disorders group [0.32 ± 0.13 (0.20–0.78), P < 0.001]. A significant difference in the mean absorbance of 65 kD hsp antigen was noted in the CSF of culture-positive TBM patients [0.94 ± 0.18 (0.54–1.29)] when compared with clinically suspected TBM patients [0.64 ± 0.20 (0.23–0.98), P < 0.05]. CONCLUSION: The presence of 65 kD hsp antigen in the CSF of confirmed and suspected cases of TBM would indicate that the selected protein is specific to M. tuberculosis and could be considered as a diagnostic marker for TBM

Improving Power Delivery of Grid-Connected Induction Machine Based Wind Generators under Dynamic Conditions Using Feedforward Linear Neural Networks

In the conventional grid-connected Wind Energy Conversion System (WECS), the generator side inverter is typically controlled via Field Oriented Control (FOC), while Voltage Oriented Control (VOC) controls the grid side inverter. However, robust operation cannot be guaranteed during sudden changes in wind speeds and weak grid connections. This paper presents a novel method to improve the overall dynamic performance of on-grid induction machine-based wind generators. An online mechanical parameter estimation technique is devised using Recursive Least Squares (RLS) to compute the machine inertia and friction coefficient iteratively. An adaptive feedforward neural (AFN) controller is also proposed in the synchronous reference frame, which is constructed using the estimated parameters and the system's inverse. The output of the neural controller is added to the output of the speed PI controller in the outer loop of the FOC to enhance the speed response of the wind generator. A similar approach is taken to improve the classical VOC structure for the grid-side inverter. In this case, the RLS estimates the equivalent Thevenin's grid impedance in real-time. As for the adaptive action, two identical neural networks are integrated with the inner loop direct and quadrature axis current PI controllers. Under nominal operating conditions, it is observed that the PI+AFN provides a faster settling time for the generator's speed and torque response. Upon being subjected to variations in the wind speed, the PI+AFN outperforms the classical PI controller and attains a lower integral-time error. In addition, the proposed PI+AFN controller has a better ability to maintain the grid-side inverter stability during stochastic variations in grid impedance. One significant advantage of the proposed control approach is that no data for training or validation is required since the neural network weights are directly the output of the RLS estimator. Hardware verification for the improved FOC for wind generators using the adaptive controller is also made using the DSPACE 1007 AUTOBOX platform

Common and unique transcriptional responses to dietary restriction and loss of insulin receptor substrate 1 (IRS1) in mice

Dietary restriction (DR) is the most widely studied non-genetic intervention capable of extending lifespan across multiple taxa. Modulation of genes, primarily within the insulin/insulin-like growth factor signalling (IIS) and the mechanistic target of rapamycin (mTOR) signalling pathways also act to extend lifespan in model organisms. For example, mice lacking insulin receptor substrate-1 (IRS1) are long-lived and protected against several age-associated pathologies. However, it remains unclear how these particular interventions act mechanistically to produce their beneficial effects. Here, we investigated transcriptional responses in wild-type and IRS1 null mice fed an ad libitum diet (WTAL and KOAL) or fed a 30% DR diet (WTDR or KODR). Using an RNAseq approach we noted a high correlation coefficient of differentially expressed genes existed within the same tissue across WTDR and KOAL mice and many metabolic features were shared between these mice. Overall, we report that significant overlap exists in the tissue-specific transcriptional response between long-lived DR mice and IRS1 null mice. However, there was evidence of disconnect between transcriptional signatures and certain phenotypic measures between KOAL and KODR, in that additive effects on body mass were observed but at the transcriptional level DR induced a unique set of genes in these already long-lived mice