Millimeter Observations of GRB 030329: Continued Evidence for a Two-Component Jet

We present the results of a dedicated campaign on the afterglow of GRB 030329 with the millimeter interferometers of the Owens Valley Radio Observatory (OVRO), the Berkeley-Illinois-Maryland Association (BIMA), and with the MAMBO-2 bolometer array on the IRAM 30-m telescope. These observations allow us to trace the full evolution of the afterglow of GRB 030329 at frequencies of 100 GHz and 250 GHz for the first time. The millimeter light curves exhibit two main features: a bright, constant flux density portion and a steep power-law decline. The absence of bright, short-lived millimeter emission is used to show that the GRB central engine was not actively injecting energy well after the burst. The millimeter data support a model, advocated by Berger et al., of a two-component jet-like outflow in which a narrow angle jet is responsible for the high energy emission and early optical afterglow, and a wide-angle jet carrying most of the energy is powering the radio and late optical afterglow emissionComment: Accepted to ApJ

SILYMARIN PROTECTS AGAINST COPPER-ASCORBATE INDUCED INJURY TO GOAT CARDIAC MITOCHONDRIA IN VITRO: INVOLVEMENT OF ANTIOXIDANT MECHANISM(S)

Silymarin, 'one of the component of the Milk thistle seeds Silybum marianum (L.) is used in traditional food and medicine in India. In the present study, we investigated the antioxidant activities of Silymarin against copper-ascorbate induced toxic injury to mitochondria obtained from goat heart, in vitro. Incubation of isolated cardiac mitochondria with copper-ascorbate resulted in elevated levels of lipid peroxidation and protein carbonylation of the mitochondrial membrane, a reduced level of mitochondrial GSH and altered status of antioxidant enzymes as well as decreased activities of pyruvate dehydrogenase and the Kreb's cycle enzymes, altered mitochondrial morphology, mitochondrial swelling and di-tyrosine level. All these changes were found to be ameliorated when the cardiac mitochondria were co-incubated with copper-ascorbate and Silymarin, in vitro. Silymarin, in our in vitro experiments, was found to scavenge hydrogen peroxide, superoxide anion free radicals, hydroxyl radicals and DPPH radical, in a chemically defined system, indicating that this compound may provide protection to cardiac mitochondria against copper-ascorbate induced toxic injury through its antioxidant activities. The results of this study suggest that Silymarin may be considered as a future therapeutic antioxidant and may be used singly or as a co-therapeutic in the treatment of diseases associated with mitochondrial oxidative stress

The Central Velocity Field in NGC 253 : Possible Indication of a Bar

We have investigated whether motion of gas in a bar-like potential can account for the peculiar but systematic velocity field observed in the nuclear region of the starburst galaxy NGC 253. This unusual velocity field with gradients along both major and minor axes was revealed in a high resolution (1.8^{\arcsec}\times 1.0^{\arcsec}) H92$\alpha$ recombination line observation by Anantharamiah and Goss (1996). A simple logarithmic potential is used to model the bar. Assuming that the bulk of the gas flows along closed and non-intersecting x1 (bar) and x2 (anti-bar) orbits of the bar potential, we have computed the expected velocity field and position-velocity diagrams and compared them with the observations. A comparison of the integrated CO intensity maps with the spatial distribution of the x1 and x2 orbits in the model indicates that the nuclear molecular gas in NGC 253 lies mainly on the x2 orbits. We also find that the velocity field observed in the central 100 pc region in the H92$\alpha$ recombination line is well accounted for by the bar model if most of the ionized gas resides in the inner x2 orbits. However, the model is unable to explain the velocity field on a larger scale of $\sim 500$ pc observed using the OVRO interferometer with a resolution of 5^{\arcsec}\times 3^{\arcsec}. The direction of the observed CO velocity field appears twisted compared to the model. We suggest that this perturbation in the velocity field may be due to an accretion event that could have occurred $10^7$ years ago.Comment: 26 latex pages, 7 figures, accepted in Astrophysical Journa

Coordinated Activation of Candidate Proto-Oncogenes and Cancer Testes Antigens via Promoter Demethylation in Head and Neck Cancer and Lung Cancer

Background: Epigenetic alterations have been implicated in the pathogenesis of solid tumors, however, proto-oncogenes activated by promoter demethylation have been sporadically reported. We used an integrative method to analyze expression in primary head and neck squamous cell carcinoma (HNSCC) and pharmacologically demethylated cell lines to identify aberrantly demethylated and expressed candidate proto-oncogenes and cancer testes antigens in HNSCC. Methodology/Principal Findings: We noted coordinated promoter demethylation and simultaneous transcriptional upregulation of proto-oncogene candidates with promoter homology, and phylogenetic footprinting of these promoters demonstrated potential recognition sites for the transcription factor BORIS. Aberrant BORIS expression correlated with upregulation of candidate proto-oncogenes in multiple human malignancies including primary non-small cell lung cancers and HNSCC, induced coordinated proto-oncogene specific promoter demethylation and expression in non-tumorigenic cells, and transformed NIH3T3 cells. Conclusions/Significance: Coordinated, epigenetic unmasking of multiple genes with growth promoting activity occurs i

Combined Forward-Backward Asymmetry Measurements in Top-Antitop Quark Production at the Tevatron

The CDF and D0 experiments at the Fermilab Tevatron have measured the asymmetry between yields of forward- and backward-produced top and antitop quarks based on their rapidity difference and the asymmetry between their decay leptons. These measurements use the full data sets collected in proton-antiproton collisions at a center-of-mass energy of $\sqrt s =1.96$ TeV. We report the results of combinations of the inclusive asymmetries and their differential dependencies on relevant kinematic quantities. The combined inclusive asymmetry is $A_{\mathrm{FB}}^{t\bar{t}} = 0.128 \pm 0.025$. The combined inclusive and differential asymmetries are consistent with recent standard model predictions

Microstructures and mechanical properties of HPT processed 6063 Al alloy

6063 Al alloy is subjected to severe plastic deformation through high-pressure torsion (HPT) using disk samples. The values of the Vickers microhardness and equivalent strain were recorded along diameters in each disk. The microhardness of 6063 Al alloys increases strongly and continuously with increasing equivalent strain but levels off and enters into a steady-state where the hardness remains unchanged with further straining. It is confirmed that the yield and ultimate tensile strength also follows the same single role of the equivalent strain as the hardness. Transmission electron microscopy showed that a subgrain structure develops at an initial stage of straining with individual grains containing disloca- tions. When increasing the straining, the subgrain size decreases whereas the misorientation angle increases and more dislocations are formed within the grains. In the steady-state range, some recrystallized grains formed which are free from dislocations. The mechanism for the grain refinement is discussed in terms of dislocation mobility

Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.

Abstract Background Di(2-ethylhexyl) phthalate (DEHP) is a widely detected plasticizer and a priority pollutant of utmost concern for its adverse impact on humans, wildlife and the environment. To eliminate such toxic burden, biological processes are the most promising ways to combat rampant environmental insults under eco-friendly conditions. The present study investigated the biochemical and molecular assessment of the catabolic potential of Mycolicibacterium sp. strain MBM in the assimilation of estrogenic DEHP. Results A detailed biochemical study revealed an initial hydrolytic pathway of degradation for DEHP followed by the assimilation of hydrolyzed phthalic acid and 2-ethylhexanol to TCA cycle intermediates. Besides the inducible nature of DEHP-catabolic enzymes, strain MBM can efficiently utilize various low- and high-molecular-weight phthalate diesters and can grow under moderately halotolerant conditions. Whole genome sequence analysis exhibited a genome size of 6.2 Mb with a GC content of 66.51% containing 6,878 coding sequences, including multiple genes, annotated as relevant to the catabolism of phthalic acid esters (PAEs). Substantiating the annotated genes through transcriptome assessment followed by RT-qPCR analysis, the possible roles of upregulated genes/gene clusters in the metabolism of DEHP were revealed, reinforcing the biochemical pathway of degradation at the molecular level. Conclusions A detailed co-relation of biochemical, genomic, transcriptomic and RT-qPCR analyses highlights the PAE-degrading catabolic machineries in strain MBM. Further, due to functional attributes in the salinity range of both freshwater and seawater, strain MBM may find use as a suitable candidate in the bioremediation of PAEs

Synthesis, characterization, X-ray crystallography and antimicrobial activities of new Co(III) and Cu(II) complexes with a pyrazole based Schiff base ligand

A new pyrazole based 'NNS' tridentate ligand, 5-methylpyrazole-3yl-N-(2'-methylthiophenyl)methyleneimine (MPzOATA), has been synthesized and characterized by elemental analyses, mass, IR and NMR spectroscopy. The versatile coordination mode of the ligand has been established with the synthesis of two metal-organic complexes with different metal ions, Co(III) (complex I) and Cu(II) (complex II). These metal-organic complexes have been characterized by single crystal X-ray structure analyses, along with several spectral techniques. Structural analyses of both the monomeric complexes reveal that complex I crystallized in the monoclinic C2/c space group, while complex II crystallized in the orthorhombic Pbca (#61) space group. In complex I, the Co(III) centre shows a six coordinate octahedral geometry, while in complex II, the Cu(II) centre shows a five coordinate square pyramidal geometry. The reported ligand and the metal ion complexes have been screened for their in vitro antimicrobial activity against some pathogenic bacteria. (C) 2013 Elsevier Ltd. All rights reserved.</p