Nanoscale electrochemical patterning reveals the active sites for catechol oxidation at graphite surfaces

Graphite-based electrodes (graphite, graphene, and nanotubes) are used widely in electrochemistry, and there is a long-standing view that graphite step edges are needed to catalyze many reactions, with the basal surface considered to be inert. In the present work, this model was tested directly for the first time using scanning electrochemical cell microscopy reactive patterning and shown to be incorrect. For the electro-oxidation of dopamine as a model process, the reaction rate was measured at high spatial resolution across a surface of highly oriented pyrolytic graphite. Oxidation products left behind in a pattern defined by the scanned electrochemical cell served as surface-site markers, allowing the electrochemical activity to be correlated directly with the graphite structure on the nanoscale. This process produced tens of thousands of electrochemical measurements at different locations across the basal surface, unambiguously revealing it to be highly electrochemically active, with step edges providing no enhanced activity. This new model of graphite electrodes has significant implications for the design of carbon-based biosensors, and the results are additionally important for understanding electrochemical processes on related sp2-hybridized materials such as pristine graphene and nanotubes

Nerve injury and neuropathic pain - A question of age

The effects of peripheral nerve injury on somatosensory processing and pain are highly dependent upon the age at which the damage occurs. Adult nerve injury rapidly triggers neuropathic pain, but this is not so if the same nerve injury is performed in animals below postnatal day (P) 28, consistent with observations in paediatric patients. However, longitudinal studies show that pain hypersensitivity emerges later in life, when the animal reaches adolescence, an observation that could be of clinical importance. Here we discuss the evidence that the central consequences of nerve damage are critically determined by the status of neuroimmune regulation at different ages. In the first postnatal weeks, when spinal somatosensory circuits are undergoing synaptic reorganisation, the 'default' neuroimmune response is skewed in an anti-inflammatory direction, suppressing the excitation of dorsal horn neurons and preventing the onset of neuropathic pain. As animals grow up and the central nervous system matures, the neuroimmune profile shifts in a pro-inflammatory direction, unmasking a 'latent' pain response to an earlier nerve injury. The data predicts that nerve injury in infancy and childhood could go unnoticed at the time, but emerge as clinically 'unexplained' or 'functional' pain in adolescence

Protecting scientists from Gordon Gekko: how organizations use hybrid spaces to engage with multiple institutional logics

Previous work on institutional complexity has discussed two solutions that organizations deploy internally when engaging externally with multiple institutional logics: blended hybrids where logics are combined throughout the organization, and structural hybrids where different logics dominate in different compartments within the organization. While blended hybrids have been extensively investigated, few studies have examined how structural hybrids are constructed and maintained. We address this imbalance by studying university-industry research centers as instances of distinct organizational spaces used to engage with a minority logic. We found that these spaces require three kinds of work: (a) leveraging, where dominant logic practices are drawn on to achieve minority logic objectives; (b) hybridizing, where the practices inside the space are modified to allow engagement with the minority logic; and (c) bolstering, where the space is shielded against excessive minority logic influence and anchored back into the organization. Furthermore, contrary to the existing literature we found that the spaces were hybrid, rather than being dominated by a single logic. Our finding is likely generalizable across many instances of structural hybrids given the integration problems that organizations with pure single logic spaces would face, combined with the usefulness of hybrid spaces. Our study is novel in revealing the work needed to sustain hybrid spaces and questioning the previously held conceptualization of structural hybrids as made up of single-logic compartments

The Time Has Come... To Move Many Things: Inventorying and Preparing a Collection for Offsite Storage

In the spring of 2019, the Montana State University (MSU) Library embarked on a large-scale inventory project that involved weeding and moving portions of their collection to an offsite storage facility within six months in order to create more student study space in the Library. The department primarily responsible for leading the project, Collections Access & Technical Services, the result of two departments merging, was also simultaneously navigating their new structure and a remodel of their workspace thus adding further challenges to the project. This poster session demonstrated how MSU Library approached and completed this project by advocating to their Library Administration for additional resources, including hiring a project manager and third-party companies to assist with the inventory and moving of the collection. It also discussed the types of work groups formed to identify new workflows (i.e., retrieval of offsite items) and modify existing ones, involving student employees in the project, and internal and external collaborations that took place. Additionally, presenters shared strategies used to communicate to their campus community, and the impact this project has had on our patrons. They also included statistics that were gathered during the project including deselection figures, the number of materials that did not have barcodes and were not accounted for in the Library’s catalog and discovery layer (Ex Libris’ Alma and Primo), and what subject areas currently remain in the main library building

Nanoscale intermittent contact-scanning electrochemical microscopy

A major theme in scanning electrochemical microscopy (SECM) is a methodology for nanoscale imaging with distance control and positional feedback of the tip. We report the expansion of intermittent contact (IC)-SECM to the nanoscale, using disk-type Pt nanoelectrodes prepared using the laser-puller sealing method. The Pt was exposed using a focused ion beam milling procedure to cut the end of the electrode to a well-defined glass sheath radius, which could also be used to reshape the tips to reduce the size of the glass sheath. This produced nanoelectrodes that were slightly recessed, which was optimal for IC-SECM on the nanoscale, as it served to protect the active part of the tip. A combination of finite element method simulations, steady-state voltammetry and scanning electron microscopy for the measurement of critical dimensions, was used to estimate Pt recession depth. With this knowledge, the tip-substrate alignment could be further estimated by tip approach curve measurements. IC-SECM has been implemented by using a piezo-bender actuator for the detection of damping of the oscillation amplitude of the tip, when IC occurs, which was used as a tip-position feedback mechanism. The piezo-bender actuator improves significantly on the performance of our previous setup for IC-SECM, as the force acting on the sample due to the tip is greatly reduced, allowing studies with more delicate tips. The capability of IC-SECM is illustrated with studies of a model electrode (metal/glass) substrate

Performance of charge-injection-device infrared detector arrays at low and moderate backgrounds

Three 2 x 64 element charge injection device infrared detector arrays were tested at low and moderate background to evaluate their usefulness for space based astronomical observations. Testing was conducted both in the laboratory and in ground based telescope observations. The devices showed an average readout noise level below 200 equivalent electrons, a peak responsivity of 4 A/W, and a noise equivalent power of 3x10 sq root of W/Hz. Array well capacity was measured to be significantly smaller than predicted. The measured sensitivity, which compares well with that of nonintegrating discrete extrinsic silicon photoconductors, shows these arrays to be useful for certain astronomical observations. However, the measured readout efficiency and frequency response represent serious limitations in low background applications

Detector arrays for low-background space infrared astronomy

The status of development and characterization tests of integrated infrared detector array technology for astronomy applications is described. The devices under development include intrinsic, extrinsic silicon, and extrinsic germanium detectors, with hybrid silicon multiplexers. Laboratory test results and successful astronomy imagery have established the usefulness of integrated arrays in low-background astronomy applications

New approaches and applications in electrochemical scanning probe microscopy

This thesis is concerned with the development of new electrochemical scanning probe techniques and the application of these to biological problems. These techniques allow high resolution quantitative investigations of surface processes through measurements at a precisely placed electrode probe. A new technique, called intermittent contact scanning electrochemical microscopy, which allowed the probe-surface distance to be decisively determined through the physical interaction of the probe with the surface was developed. Separately, a new type of dual electrode probe was developed and characterised, and a new instrument (including both hardware and software) capable of a wide range of electrochemical imaging modes was developed with wide applications. The quantitative analysis of the electrochemical signal, typically measured at the probe, requires understanding the mass transport between the probe and the surface. Finite element modelling was used extensively throughout to solve the mass transport problem and therefore quantitatively analyse experimental results. Intermittent contact scanning electrochemical microscopy was used to quantify the mass transport through a porous biological membrane, dentin, that separates the pulp and enamel in teeth. Oxygen generation and consumption rates during photosynthesis were determined by measuring the local oxygen flux at an electrode placed a precise distance above a monolayer of isolated chloroplasts or thylakoid membranes. Finally, the new dual electrode probe was used to measure the reduction of an artificial electron acceptor by isolated thylakoid membranes