Role of product characteristics for the adoption of fruit and fruit product innovations

The aim of this study was the identification of those product characteristics that are important for the adoption of fruit and fruit product innovations by consumers. Sixteen focus group discussions were held in four European countries (Greece, The Netherlands, Poland, and Spain). Different aspects of six innovative fruit products were discussed, revealing those characteristics that were important for the adoption of each of them. It was observed that the participants did not perceive fruit innovations as a homogenous group, but assigned them to different groups, which led to a number of categories of fruit innovation. Three categories concerned the level of preparation of fruit. These were fresh, prepared, and processed fruit product innovations. Another two categories, radical and evolutionary innovations, related to the level of novelty of the fruit innovation. Characteristics important for the adoption of each of these categories are given.The results will be used for further, more quantitative, research

The use of secondary school student ratings of their teacher’s skillfulness for low-stake assessment and high-stake evaluation

Previous studies in higher education have shown that the reliability of student ratings of teaching skill increases if multiple ratings by different students are aggregated. This study examines the generalizability of these findings to the context of secondary education. Also, it seeks to validate these findings by comparing reliability levels estimated by the routinely used nested design with those estimated using a more complex design. The sample consisted of 410 students from 17 classes rating 63 teachers working at eight schools across the Netherlands. Using the nested design, the study replicates findings of previous studies in higher education. The findings illustrate how the reliability level of secondary school students’ ratings increases with an increasing number of students. However, these replicated reliability levels were not validated by the more complex design which provided lower estimates. This indicates that the nested design may not provide accurate estimations of rating reliability

Factors determining a successful socioeconomic introduction of horticulture in foreign countries - Academic Consultancy Training Report

Course: Academic Consultancy Training (YMC 60809) Project: Sustainable development of greenhouse horticulture in developing countries (756) Commissioner: Wageningen UR Greenhouse Horticulture Contact person: Ir. C.J.M. van der Lans Coach: Dr. Ir. J.W. Hofstee Expert: Prof. Dr. O. van Kooten A lot of capital is attracted in the initiation of Dutch horticulture businesses abroad. There is however a lack of knowledge about what is known about the critical factors determining success or failure of a horticultural initiative in a foreign country. The goal of this project is to achieve socio-economic sustainability of the greenhouse horticulture in foreign countries. Therefore a model is presented which contains all the necessary factors that an entrepreneur should keep in mind to achieve socio-economic sustainability. In order to get an overview of all the important factors literature was studied and interviews were conducted. This led to a detailed description of all the issues that should be considered when setting-up businesses abroad. Those factors are represented in a ‘dial’ model pointing out what issues an entrepreneur should consider. The model is subdivided in three topics: market, production and trade & logistics. Important factors related to the market are whether there is a market and how to influence the whole production chain. Important factors for the production are the physical place and the employees. A very important factor of the trading is the legalization and the bureaucracy of the country you are producing in

Structural insights into the synthesis of FMN in prokaryotic organisms

Riboflavin kinases (RFKs) catalyse the phosphorylation of riboflavin to produce FMN. In most bacteria this activity is catalysed by the C-terminal module of a bifunctional enzyme, FAD synthetase (FADS), which also catalyses the transformation of FMN into FAD through its N-terminal FMN adenylyl transferase (FMNAT) module. The RFK module of FADS is a homologue of eukaryotic monofunctional RFKs, while the FMNAT module lacks homologyto eukaryotic enzymes involved in FAD production. Previously, the crystal structure of Corynebacterium ammoniagenes FADS (CaFADS) was determined in its apo form. This structure predicted a dimer-of-trimers organization with the catalytic sites of two modules of neighbouring protomers approaching each other, leading to a hypothesis about the possibility of FMN channelling in the oligomeric protein. Here, two crystal structures of the individually expressed RFK module of CaFADS in complex with the products of the reaction, FMN and ADP, are presented. Structures are complemented with computational simulations, binding studies and kinetic characterization. Binding of ligands triggers dramatic structural changes in the RFK module, which affect large portions of the protein. Substrate inhibition and molecular-dynamics simulations allowed the conformational changes that take place along the RFK catalytic cycle to be established. The influence of these conformational changes in the FMNAT module is also discussed in the context of the full-length CaFADS protomer and the quaternary organization.This work has been supported by MINECO, Spain (BIO2013-42978-P to MM and BFU2014-59389-P to JAH), the Aragonian Government-FEDER (B18), Autonomous Community of Madrid (S2010/BMD-2457), Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) and Universidad Industrial de Santander (project 1818 to IL).Peer Reviewe

Entrepreneurship Education and Training in a Small Business Context: Insights from the Competence-based Approach

The concept of competence, as it is brought into play in current research, is a potentially powerful construct for entrepreneurship education research and practice. Although the concept has been the subject of strong debate in educational research in general, critical analysis of how it has been used, applied and experienced in entrepreneurship education practice is scarce. This article contributes specifically to the discussion of entrepreneurial competence by theoretically unfolding and discussing the concept. Subsequently, the implications of applying a competence-based approach in entrepreneurship education are illustrated and discussed based on analysis of two cases t

On the "association between two things":the case of student surveys and classroom observations of teaching quality

This study examines the association between two measures of teaching effectiveness—a student survey measure and a classroom observation measure—to determine whether their correlation depends on the study design. The sample includes 160 classroom observations of 56 teachers across 15 classes, in which students also rated the teachers with a survey. Dependencies are examined using generalizability theory. Results suggest that the correlation between the survey and observation measures depends on the number of classroom observations, the number of student ratings, and whether the designs are nested or partially nested. The effect is substantial: Predicted correlations range between 0.10–0.80 for the same variables with different study designs. In particular, the number of classroom observations has a notable influence, such that across all investigated scenarios, the correlation doubles when observers visit three lessons instead of one. Correlations also tend to be positively biased when research designs are nested

Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues

Transcription-coupled nucleotide excision repair (TC-NER) is an important DNA repair mechanism that protects against the negative effects of transcription-blocking DNA lesions. Hereditary TC-NER deficiencies cause pleiotropic and often severe neurodegenerative and progeroid symptoms. While multiple assays have been developed to determine TC-NER activity for clinical and research purposes, monitoring TC-NER is hampered by the low frequency of repair events occurring in transcribed DNA. ’Recovery of RNA Synthesis’ is widely used as indirect TC-NER assay based on the notion that lesion-blocked transcription only resumes after successful TC-NER. Here, we show that measuring novel synthesis of a protein after its compound-induced degradation prior to DNA damage induction is an equally effective but more versatile manner to indirectly monitor DNA repair activity in transcribed genes. This ‘Recovery of Protein Synthesis’ (RPS) assay can be adapted to various degradable proteins and readouts, including imaging and immunoblotting. Moreover, RPS allows real-time monitoring of TC-NER activity in various living cells types and even in differentiated tissues of living organisms. To illustrate its utility, we show that DNA repair in transcribed genes declines in aging muscle tissue of C. elegans. Therefore, the RPS assay constitutes an important novel clinical and research tool to investigate transcription-coupled DNA repair