Diplomacy in the Time of Cholera

Turning everyday ordinary happenings into struggling moments for existence—from breathing to socializing—is how the Covid-19 pandemic will mark history. What we ask here is not how the ordinary becomes abnormal but how it becomes political and diplomatic. We argue that the spread of the Covid-19 virus, which is measured through virologic and epidemiological models, overlaps with feverous diplomatic and political activities taking place among big geopolitical powers. Yet, this is not new in history of health. The first encounters between diplomats and health professionals were elicited by the social and economic challenges caused, on a global scale, by the cholera epidemics of the nineteenth century. Indeed, health sciences and diplomacy have been historically co-produced. Such a historical perspective on science and health diplomacy facilitates our understanding of international institutions such as the World Health Organization as highly political and diplomatic endeavors. The Diplomatic Studies of Science, a new interdisciplinary research field underpinned by a historical perspective on science diplomacy, sheds light on the multiple factors contributing to the worsening of the global COVID-19 crisis we are facing nowadays

N-terminal and C-terminal domains of calmodulin mediate FADD and TRADD interaction

FADD (Fas–associated death domain) and TRADD (Tumor Necrosis Factor Receptor 1-associated death domain) proteins are important regulators of cell fate in mammalian cells. They are both involved in death receptors mediated signaling pathways and have been linked to the Toll-like receptor family and innate immunity. Here we identify and characterize by database search analysis, mutagenesis and calmodulin (CaM) pull-down assays a calcium-dependent CaM binding site in the α-helices 1–2 of TRADD death domain. We also show that oxidation of CaM methionines drastically reduces CaM affinity for FADD and TRADD suggesting that oxidation might regulate CaM-FADD and CaM-TRADD interactions. Finally, using Met-to-Leu CaM mutants and binding assays we show that both the N- and C-terminal domains of CaM are important for binding

Distal unfolding of ferricytochrome C induced by the F82K mutation

It is well known that axial coordination of heme iron in mitochondrial cytochrome c has redox-dependent stability. The Met80 heme iron axial ligand in the ferric form of the protein is relatively labile and can be easily replaced by alternative amino acid side chains under non-native conditions induced by alkaline pH, high temperature, or denaturing agents. Here, we showed a redox-dependent destabilization induced in human cytochrome c by substituting Phe82\u2014conserved amino acid and a key actor in cytochrome c intermolecular interactions\u2014with a Lys residue. Introducing a positive charge at position 82 did not significantly affect the structure of ferrous cytochrome c but caused localized unfolding of the distal site in the ferric state. As revealed by1 H NMR fingerprint, the ferric form of the F82K variant had axial coordination resembling the renowned alkaline species, where the detachment of the native Met80 ligand favored the formation of multiple conformations involving distal Lys residues binding to iron, but with more limited overall structural destabilization

Teaching Comparative and International Education in the UK: a national survey of undergraduate programmes

This BAICE-funded study reports on the status of comparative and international education (CIE) in undergraduate programmes in the UK based on analysis of curriculum documents and interviews with teachers on CIE units. While there are have been numerous efforts to map CIE research over the years (Davidson et al., 2020; Pizmony-Levy, 2021), the nature of CIE teaching has received considerably less attention. To our knowledge this is the first systematic effort to map CIE teaching in UK universities since the 1990s (Schweisfurth 1999). The overarching question guiding this study is the nature of CIE as taught at undergraduate level in the UK, as well as the value and the challenges of such courses from the perspectives of teachers. We explore these issues through the analysis of curriculum documents and interviews with teachers. Evidence for this study was collected as follows. In September 2020 we issued a call via the BAICE mailing list, social media and professional networks for UK-based colleagues to share the handbooks for undergraduate courses which address comparative education, international education and/or globalisation. Materials for 40 units (Appendix 1) were received from colleagues at 24 institutions (9 pre-1992, 15 post-1992). Following this, in Spring 2021 interviews were conducted with seven teachers of CIE (4 pre-1992, 3 post-1992). In this report we share findings on the geographical and thematic coverage of CIE at undergraduate level, and teachers’ perspectives on the purposes as well as the challenges of teaching these courses. We hope that findings from this study can inform future teaching, curriculum development and debate in this area

Taking a global perspective:The nature and value of comparative and international education (CIE) in UK universities

Comparative and international education (CIE) offers undergraduate students a perspective on education systems and contexts beyond their own direct experience. In this chapter, we introduce CIE as a field, and share an overview of what is currently taught within CIE units and modules at undergraduate level in the UK. To do so, we draw on a review of CIE units at 24 UK universities – perhaps even including your own institution, and units that you may have taken or consider taking in the future. Our findings demonstrate some key current debates within the field and push readers to think more deeply about the values, priorities and assumptions that we may take for granted in studying others’ approaches to education

Timing and modulation of activity in the lower limb muscles during indoor rowing: What are the key muscles to target in FES-rowing protocols?

The transcutaneous stimulation of lower limb muscles during indoor rowing (FES Rowing) has led to a new sport and recreation and significantly increased health benefits in paraplegia. Stimulation is often delivered to quadriceps and hamstrings; this muscle selection seems based on intuition and not biomechanics and is likely suboptimal. Here, we sample surface EMGs from 20 elite rowers to assess which, when, and how muscles are activated during indoor rowing. From EMG amplitude we specifically quantified the onset of activation and silencing, the duration of activity and how similarly soleus, gastrocnemius medialis, tibialis anterior, rectus femoris, vastus lateralis and medialis, semitendinosus, and biceps femoris muscles were activated between limbs. Current results revealed that the eight muscles tested were recruited during rowing, at different instants and for different durations. Rectus and biceps femoris were respectively active for the longest and briefest periods. Tibialis anterior was the only muscle recruited within the recovery phase. No side differences in the timing of muscle activity were observed. Regression analysis further revealed similar, bilateral modulation of activity. The relevance of these results in determining which muscles to target during FES Rowing is discussed. Here, we suggest a new strategy based on the stimulation of vasti and soleus during drive and of tibialis anterior during recovery

Integrative analysis of SF-1 transcription factor dosage impact on genome-wide binding and gene expression regulation

Steroidogenic Factor-1 (SF-1) is a nuclear receptor that has a pivotal role in the development of adrenal glands and gonads and in the control of steroid hormone production, being also implicated in the pathogenesis of adrenocortical tumors. We have analyzed the mechanisms how SF-1 controls gene expression in adrenocortical cells and showed that it regulates different categories of genes according to its dosage. Significant correlations exist between the localization of SF-1-binding sites in chromatin under different dosage conditions and dosage-dependent regulation of gene expression. Our study revealed unexpected functional interactions between SF-1 and Neuron-Restrictive Silencer Factor/RE1-Silencing Transcription Factor (NRSF/REST), which was first characterized as a repressor of neuronal gene expression in non-neuronal tissues, in the regulation of gene expression in steroidogenic cells. When overexpressed, SF-1 reshapes the repertoire of NRSF/REST—regulated genes, relieving repression of key steroidogenic genes. These data show that NRSF/REST has a novel function in regulating gene expression in steroidogenic cells and suggest that it may have a broad role in regulating tissue-specific gene expression programs

Surprising Complexity of the [Gd(AAZTA)(H2O)2]- Chelate Revealed by NMR in the Frequency and Time Domains

Typically, Ln(III) complexes are isostructural along the series, which enables studying one particular metal chelate to derive the structural features of the others. This is not the case for [Ln(AAZTA)(H2O)x]- (x = 1, 2) systems, where structural variations along the series cause changes in the hydration number of the different metal complexes, and in particular the loss of one of the two metal-coordinated water molecules between Ho and Er. Herein, we present a 1H field-cycling relaxometry and 17O NMR study that enables accessing the different exchange dynamics processes involving the two water molecules bound to the metal center in the [Gd(AAZTA)(H2O)2]- complex. The resulting picture shows one Gd-bound water molecule with an exchange rate ∼6 times faster than that of the other, due to a longer metal-water distance, in accordance with density functional theory (DFT) calculations. The substitution of the more labile water molecule with a fluoride anion in a diamagnetic-isostructural analogue of the Gd-complex, [Y(AAZTA)(H2O)2]-, allows us to follow the chemical exchange process by high-resolution NMR and to describe its thermodynamic behavior. Taken together, the variety of tools offered by NMR (including high-resolution 1H, 19F NMR as a function of temperature, 1H longitudinal relaxation rates vs B0, and 17O transverse relaxation rates vs T) provides a complete description of the structure and exchange dynamics of these Ln-complexes along the series

Cms gem detector material study for the hl-lhc

A study on the Gaseous Electron Multiplier (GEM) foil material is performed to determine the moisture diffusion rate, moisture saturation level and the effects on its mechanical properties. The study is focused on the foil contact with ambient air and moisture to determine the value of the diffusion coefficient of water in the foil material. The presence of water inside the detector foil can determine the changes in its mechanical and electrical properties. A simulated model is developed with COMSOL Multiphysics v. 4.3 [1] by taking into account the real GEM foil (hole dimensions, shapes and material), which describes the adsorption of water. This work describes the model, its experimental verification, the water diffusion within the entire sheet geometry of the GEM foil, thus gaining concentration profiles and the time required to saturate the system and the effects on the mechanical properties