10 research outputs found

    Review Article: Molecular Mechanisms of Resistance to Potato virus X and Y in Potato

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    The most important viral pathogens of the cultivated potato are Potato virus X (PVX) and Potato virus Y (PVY), which can reduce potato production up to 80%. Thus resistance breeding is one of the major goals of plant breeders. Wild potato species are good sources of resistance (R) genes. The resistant plants respond to viral infection with hypersensitive reaction (HR) or extreme resistance (ER). HR is accompanied by programmed cell death, while ER localizes the virus at the primary infection site and limits virus replication without visible symptoms. While HR is generally strain-specific, ER can act against a broad spectrum of viral pathogens. This review aims to describe the molecular mechanisms of resistance against PVX and PVY in potato

    Morphological and RAPD analysis of poplar trees of anther culture origin.

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    Our objectives were to improve the rate of haploid plant regeneration through increasing the rate of callus initiation on the anthers and sustaining shoot regeneration frequency, and to analyze the field population of anther culture origin by morphological and molecular methods. Regarding the callus initiation, the most responsive clones were 'N-90' (59%) in P. nigra and 'D-29' (75%) in P. deltoides. The rate of shoot regeneration and number of shoots/calli ranged from 4%-79% and 1-9, respectively. From the 208 rooted plants 8 haploid, 179 diploid, 4 tetraploid and 17 aneuploid plants were found. In the field population, the haploid plants could be easily identified by their retarded development and morphological characteristics (size and shape of the leaves, strong branching, etc). Several diploid plants showed depressed developmental and morphological traits similar to the haploid ones. Three traits (growth rate, leaf blade length and shape of leaf base) of the 6 different morphological characteristics measured were in correlation with the ploidy level within the poplar field population. Six primers of the 48 primers tested were able to detect polymorphism among the field plants

    Less is more: strategies to remove marker genes from transgenic plants

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