Massive fish mortality in Ismarida Lake, Greece: identification of drivers contributing to the fish kill event

During the end of August 2013, a massive fish mortality occurred in Ismarida Lake, a small and shallow system of Northern Greece, where approximately 10-18 tons of euryhaline fish died. This study attempts to describe the event of this fish kill or Massive Fish Mortality (MFM) that occurred in Ismarida Lake during the night of August 28, 2013, and to identify the possible drivers that may have triggered this event. A combined hydrographic, ichthyological and phytoplankton survey were carried out along with a toxicological analysis. Finally, the study proposes both short-term and long-term measures for the management of both quality and quantity of the water (ground and surface) resources in the broader basin of Ismarida Lake

Monitoring of cyanobacteria for water quality: doing the necessary right or wrong?

Cyanobacteria are an essential biological component of phytoplankton water quality assessment. However, there are some problems associated with the widely used everyday practices of sampling, estimation and use of cyanobacteria when calculating phytoplankton indices assessing water quality. Many indices were developed during the implementation of the Water Framework Directive, considered the most innovative European environmental legislation. Most indices include cyanobacteria as a composition or bloom metric. Problems with the indices concern the exclusion of most chroococcalean taxa from cyanobacterial biovolume estimations in lakes and reservoirs of the Mediterranean region, treatment of the mucilage of colonial chroococcalean taxa in biovolume estimations and overlooking of deep-water cyanobacterial blooms due to sampling depth. These problems may lead to a biased view of water quality. In this paper we argue in favour of including all cyanobacteria taxa and their mucilage in biovolume estimations and considering a sampling depth that covers deep-water maxima, such as those formed by Planktothrix rubescens or colonial chroococcalean taxa

Phytoplankton and water quality in a Mediterranean drinking-water reservoir (Marathonas Reservoir, Greece)

Phytoplankton and water quality of Marathonas drinking-water Reservoir were examined for the first time. During the study period (July-September 2007), phytoplankton composition was indicative of eutrophic conditions although phytoplankton biovolume was low (max. 2.7 mm(3) l (-aEuro parts per thousand 1)). Phytoplankton was dominated by cyanobacteria and diatoms, whereas desmids and dinoflagellates contributed with lower biovolume values. Changing flushing rate in the reservoir (up to 0.7% of reservoir's water volume per day) driven by water withdrawal and occurring in pulses for a period of 15-25 days was associated with phytoplankton dynamics. Under flushing pulses: (1) biovolume was low and (2) both 'good' quality species and the tolerant to flushing 'nuisance' cyanobacterium Microcystis aeruginosa dominated. According to the Water Framework Directive, the metrics of phytoplankton biovolume and cyanobacterial percentage (%) contribution indicated a moderate ecological water quality. In addition, the total biovolume of cyanobacteria as well as the dominance of the known toxin-producing M. aeruginosa in the reservoir's phytoplankton indicated a potential hazard for human health according to the World Health Organization

Different phytoplankton descriptors show asynchronous changes in a shallow urban lake (L. Kastoria, Greece) after sewage diversion

Phytoplankton long-term changes were examined in a shallow urban lake (L. Kastoria, Greece) after sewage diversion in nine distinct years from 1994-2010. Using different levels of phytoplankton descriptors (diversity, taxonomic and functional composition, dominant species, total phytoplankton and cyanobacterial biomass) asynchronous changes were observed in the lake's phytoplankton community. The first dynamic response was observed five years after sewage diversion by a sharp decrease in total phytoplankton and cyanobacterial biomass. However, cyanobacteria remained the predominant taxonomic group. Only in 2005, a shift from cyanobacteria to chlorophytes-diatoms was observed, coinciding with a considerable decrease in the lake's water level due to flushing. A depletion of the long-lasting dominant filamentous cyanobacteria Limnothrix redekei and Cylindrospermopsis raciborskii was observed from 2005-2010. In contrast, a persistent dominance of toxic Microcystis species was recorded, indicating insufficient water quality. Fifteen years after sewage diversion, phytoplankton species richness doubled and functional diversity increased to include newly established species. Overall, the presented data provide insights into how the phytoplankton community assembles during restoration, with implications both for scientific analysis and ecological water quality management in the Mediterranean region

Molecular diversity of bacteria in commercially available "Spirulina" food supplements

The cyanobacterium Arthrospira is among the most well-known food supplements worldwide known as "Spirulina." While it is a widely recognized health-promoting natural product, there are no reports on the molecular diversity of commercially available brands of "Spirulina" supplements and the occurrence of other cyanobacterial and heterotrophic bacterial microorganisms in these products. In this study, 454- pyrosequencing analysis of the total bacterial occurrence in 31 brands of "Spirulina" dietary supplements from the Greek market was applied for the first time. In all samples, operational taxonomic units (OTUs) of Arthrospira platensis were the predominant cyanobacteria. Some products contained additional cyanobacterial OTUs including a few known potentially toxic taxa. Moreover, 469 OTUs were detected in all 31 products collectively, with most of them being related to the Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria and Verrucomicrobia. All samples included heterotrophic bacterial OTUs, ranging from 9-157 per product. Among the most common OTUs were ones closely related to taxa known for causing health issues (i.e., Pseudomonas, Flavobacterium, Vibrio, Aeromonas, Clostridium, Bacillus, Fusobacterium, Enterococcus). The observed high cyanobacterial and heterotrophic bacterial OTUs richness in the final product is a point for further research on the growth and processing of Arthrospira biomass for commercial purposes. © 2016 Vardaka et al

Artificially-born "killer" lake: Phytoplankton based water quality and microcystin affected fish in a reconstructed lake

Lake Karla (Greece) is an example of a lake ecosystem which was dried in 1960's and now is restored, facing various anthropogenic pressures, whereas it is also listed in the network of Greek protected areas in terms of its conservation value. The objective of the present study was to determine the presence of microcystins (MCYST) in the lake water and their accumulation in tissues of the commercial fish species Cyprinus carpio, along with the highlighting of phytoplankton community and general limnological features of Lake Karla, a newly reconstructed lake, the first year of its refilling. MCYST concentrations in water and fish tissues were determined by an enzyme-linked immunosorbent assay (ELISA). Results suggest that Lake Karla has undergone a progressive cultural eutrophication with frequent cyanobacterial blooms. The most dominant species in lake's phytoplankton were Anabaenopsis elenkinii, Sphaerospermopsis and Planktothrix agardhii. MCYST concentrations were detected in water samples comparable to those reported for other eutrophicated Mediterranean lakes while considerable amounts of MCYST were detected in the tissues of the species C carpio in the following order: liver>kidney>brain>intestine>muscles. The presence of prominent cyanobacterial blooms dominated by toxic species highlights the need to undertake eutrophication control measures so as to avoid further toxicological problems. (C) 2013 Elsevier B.V. All rights reserved

Variability of airborne bacteria in an urban Mediterranean area (Thessaloniki, Greece)

The abundance, biomass and the taxonomic composition of the total airborne bacterial communities in a coastal urban area of Northeastern Mediterranean Sea were examined. In total, 27 air samples were collected across three seasons from a sampling point of approximately 30 m altitude in the center of the city. The abundance and biomass were determined with the use of epifluorescent microscopy, while the taxonomic composition was characterized by next-generation sequencing methods. Overall, the highest values of bacterial abundance were recorded during summer, with values exceeding abundances recorded in other urban sites across Europe, reaching 41 × 104 cells m−3. Out of 6 core meteorological parameters, only air temperature was found to significantly affect the abundance and biomass of airborne bacteria. Concerning the taxonomic composition of the airborne bacterial community, the group of Proteobacteria was the most diverse, with 47% of the total number of OTUs belonging to them, followed by Firmicutes, Actinobacteria and Bacteroidetes. The most dominant OTU belonged to γ-Proteobacteria, and was closely affiliated to Pseudomonas sp., a taxon commonly found to actively participate in the formation of ice-nuclei in the atmosphere. Finally, 19 OTUs were shared between all seasons and were found to be among the most dominant overall. The majority of these OTUs were affiliated to genera from soil and wastewater origin, while several were affiliated to genera that include known or opportunistic pathogens. Yet, only rare OTUs were affiliated to taxa with possible marine origin (e.g. Synechococcus sp.). The results showed that the atmosphere of the study area harbors a diverse and abundant bacterial community. © 2017 Elsevier Lt

Raphidiopsis mediterranea Skuja represents non-heterocytous life-cycle stages of Cylindrospermopsis raciborskii (Woloszynska) Seenayya et Subba Raju in Lake Kastoria (Greece), its type locality: Evidence by morphological and phylogenetic analysis

The taxonomical relationship of Cylindrospermopsis raciborskii and Raphidiopsis mediterranea was studied by morphological and 16S rRNA gene diversity analyses of natural populations from Lake Kastoria, Greece. Samples were obtained during a bloom (23,830 trichomes mL(-1)) in August 2003. A high diversity of apical cell, trichome, heterocyte and akinete morphology, trichome fragmentation and reproduction was observed. Trichomes were grouped into three dominant morphotypes: the typical and the non-heterocytous morphotype of C. raciborskii and the typical morphotype of R. mediterranea. A morphometric comparison of the dominant morphotypes showed significant differences in mean values of cell and trichome sizes despite the high overlap in the range of the respective size values. Additionally, two new morphotypes representing developmental stages of the species are described while a new mode of reproduction involving a structurally distinct reproductive cell is described for the first time in planktic Nostocales. A putative life-cycle, common for C. raciborskii and R. mediterranea is proposed revealing that trichome reproduction of R. mediterranea gives rise both to R. mediterranea and C. raciborskii non-heterocytous morphotypes. The phylogenetic analysis of partial 16S rRNA gene (ca. 920 bp) of the co-existing Cylindrospermopsis and Raphidiopsis morphotypes revealed only one phylotype which showed 99.54% similarity to R. mediterranea HB2 (China) and 99.19% similarity to C. raciborskii form 1 (Australia). We propose that all morphotypes comprised stages of the life cycle of C. raciborkii whereas R. mediterranea from Lake Kastoria (its type locality) represents non-heterocytous stages of Cylindrospermopsis complex life cycle. (C) 2009 Elsevier B.V. All rights reserved