The human glucocorticoid receptor as an RNA-binding protein: global analysis of glucocorticoid receptor-associated transcripts and identification of a target RNA motif.

Posttranscriptional regulation is emerging as a key factor in glucocorticoid (GC)-mediated gene regulation. We investigated the role of the human GC receptor (GR) as an RNA-binding protein and its effect on mRNA turnover in human airway epithelial cells. Cell treatment with the potent GC budesonide accelerated the decay of CCL2 mRNA (t(1/2) = 8 ± 1 min versus 62 ± 17 min in DMSO-treated cells) and CCL7 mRNA (t(1/2) = 15 ± 4 min versus 114 ± 37 min), but not that of CCL5 mRNA (t(1/2)=231 ± 8 min versus 266 ± 5 min) in the BEAS-2B cell line. This effect was inhibited by preincubation with an anti-GR Ab, indicating that GR itself plays a role in the turnover of these transcripts. Coimmunoprecipitation and biotin pulldown experiments showed that GR associates with CCL2 and CCL7 mRNAs, but not CCL5 mRNA. These methods confirmed CCL2 mRNA targeting by GR in human primary airway epithelial cells. Association of the GR was localized to the 5' untranslated region of CCL2 mRNA and further mapped to nt 44-60. The collection of transcripts associated with GR, identified by immunoprecipitation of GR-mRNA complexes followed by microarray analysis, revealed 479 transcripts that associated with GR. Computational analysis of the primary sequence and secondary structures of these transcripts yielded a GC-rich motif, which was shown to bind to GR in vitro. This motif was used to predict binding of GR to an additional 7889 transcripts. These results indicate that cytoplasmic GR interacts with a subset of mRNA through specific sequences and can regulate turnover rates, suggesting a novel posttranscriptional role for GR as an RNA-binding protein

Steak Location Within the Semitendinosus Muscle Impacts Metmyoglobin Accumulation on Steaks During Retail Display

Beef color is a major attribute consumers utilize to make purchasing decisions. It is estimated poor color shelf-life of beef steaks costs the meat industry more than $1 billion annually. Shelf-life color is influenced by a balance of two biochemical processes within steaks: metmyoglobin reducing ability and oxygen consumption. Steaks that exhibit a greater metmyoglobin reducing and a reduced oxygen consumption are typically characterized as more color stable. Characteristics of the muscle fiber or muscle cell are what determine the properties of a steak. Commonly, muscles with more oxidative fibers have an elevated oxygen consumption and reduced metmyoglobin reducing ability. The Semitendinosus muscle or eye of round possesses a divergent muscle fiber isoform distribution based on the location steaks are fabricated. The objective of this study was to examine effects of steak location on muscle fiber type distribution and metmyoglobin accumulation of Semitendinosus steaks

Coarse Marbled Beef is Juicier and More Flavorful Than Fine or Medium Marbled Beef

Beef palatability and eating experience is driven primarily by U.S. Department of Agriculture quality grade and marbling levels. Beef USDA quality grade consists of both marbling levels and maturity. Conventionally, marbling texture has not been a consideration of quality grades. Currently, only one study has assessed the effects of marbling texture on beef palatability. Despite this, preferences for fine or medium marbling exist with both packers and retailers, as approximately 75% of branded beef programs under the supervision of USDA-AMS require fine or medium textured marbling, which equates to losses of premiums for packers and producers (USDA, 2016). The objective of the study was to evaluate the effects of marbling texture on trained sensory panel ratings of beef strip loin steaks of varying USDA quality grades and marbling textures

Marbling Texture Does Not Affect Consumer Preference of Beef Strip Loin Steaks

In the beef industry, U.S. Department of Agriculture quality grades and marbling levels have long been associated with beef palatability and eating experience. Marbling score and maturity are the two major components of USDA quality grade. Traditionally, marbling texture has not been considered a factor of marbling score; however, there are often discernments at both the packer and retail level, as more than 75% of branded beef programs supervised by USDA-AMS have a specification of fine or medium textured marbling (USDA, 2015). Additionally, in some cases, fine and medium textured steaks are graded higher than their coarse counterparts, which results in a loss of possible premiums for producers and packers. There is very little research evaluating marbling texture and its effect on palatability and eating experience

How do Alternative Pressures Affect the Accuracy of the Pressed Juice Percentage (PJP) at Predicting Consumer Juiciness Rating?

Texture is the most important sensory trait when evaluating meat products (Gomes et al., 2014). In beef products, tenderness and juiciness interact to form overall texture and mouthfeel. As a result, beef juiciness is one of the most important factors in creating a satisfactory beef eating experience. A recent study by Woolley (2014) developed an instrumental technique for measuring beef juiciness and predicting consumer beef juiciness satisfaction. The method utilizes a texture analyzer to compress cooked beef samples for a period of 8 seconds at 17.6 lb of force and quantifies the percentage of moisture lost as Pressed Juice Percentage (PJP), a predictor of beef juiciness. Results from the initial study found PJP accounts for 20% of the variation in consumer juiciness scores; however, an evaluation of additional pressures is needed to determine if the PJP method can account for an even greater percentage of variation. Thus, the objective of this study was to evaluate the PJP method utilizing three different pressures and determine the relationship of these values to consumer sensory scores for juiciness

Palatability of Ground Beef Increases When Brand Is Disclosed in Consumer Testing

Of the beef products on the market, ground beef is one of the least expensive and most universal. Ground beef represents the largest volume of protein served in the foodservice industry, at 64%, and is the most popular beef item for consumers when preparing meals in their home (NCBA, 2012). To date, little research has evaluated ground beef palatability despite representing a large sector of the beef market. All ground beef is not the same to consumers. Ground beef from branded beef programs, higher lean points, and primal-specific blends are traditionally sold at retail for higher prices. Branding is used to indicate an increased quality level associated with the product, and encourages consumers to pay a premium in order to receive a superior product (Grunert et al., 2004). Certain branding strategies can influence consumers’ purchasing decisions even if they have not previously tasted the product (Levin and Gaeth, 1988). There is no conclusive evidence of how ground beef palatability changes with fat levels, although some studies have indicated products with higher fat levels are perceived to be more juicy (Myers et al., 2012). It is common for meat product studies to evaluate palatability differences in products through blind testing; however, consumers do not purchase and consume meat without being exposed to information about the product. Therefore, the objective of this study was to determine the effect of brand and product identification on consumer palatability ratings of ground beef patties

Pressed Juice Percentage Can Accurately Sort Beef into Categories of Predicted Juiciness

There are three main factors that contribute to meat palatability: tenderness, juiciness, and flavor (Bratzler, 1971). These three individual factors all play a role in the overall palatability perceived by a consumer. If a product fails for juiciness, there is a greater chance that it will fail in overall acceptability (Emerson et. al, 2013). In the past, research has established a method of segregating steaks based on tenderness acceptability. Researchers have been able to institute thresholds to be able to accurately explain at what shear force a steak will be rated tender by consumers. Similar methods have not been evaluated for juiciness until very recently when Woolley (2014) developed a method to objectively quantify beef juiciness. The method that was created included calculating the percentage of moisture loss from each sample after being compressed. From this work, thresholds for juiciness acceptability were established using loigistic regression; however, additional research is needed to verify these established thresholds. Therefore, the objective of this study was to determine the accuracy of previously established threshold values by testing consumer juiciness ratings for beef steaks in relation to objective juiciness measures

Does Knowing Brand or USDA Grade of Beef Strip Steaks Affect Palatability for Consumers?

In consumers’ eyes, not all beef is considered equal. In addition to USDA quality grades, close to 150 branded beef programs are approved for the segregation and marketing of beef products (USDA, 2015). This large number of product categories allows consumers to have a choice in the products they purchase. Blind sensory panel testing of beef, where consumers are not shown the brand or information about a product, has been used for many years. While important to determine palatability characteristics of beef when evaluated blind, consumers do not select, purchase, and consume beef without additional product information. Evidence suggests that branding and product labeling has an influence on consumers’ decisions before having firsthand experience of the product (Levin and Gaeth, 1988). Branding products allows pieces of information to be used to form quality expectations (Steenkamp and vanTrijp, 1996) and can encourage consumers to pay a premium for the increased quality associated with a brand (Grunert et al., 2004). Moreover, previous research suggests that consumers perceive a product differently when brand information is disclosed (Allison and Uhl, 1964). Numerous studies have evaluated the economic impact of beef branding; however, no research has focused on the effect of branding on consumer perception of beef eating quality. Therefore, our objective was to determine how consumer palatability ratings of beef strip loin steaks are affected when products are identified with a brand or USDA grade

The Effect of Enhancement on Trained Panel Beef Palatability Scores Is Dependent Upon USDA Quality Grade

Quality grades are used to determine beef value. The U.S. Department of Agriculture grading system categorizes beef into levels of eating satisfaction with the highest being Prime and decreases until reaching the Canner quality grade. Currently the premium of Prime graded carcasses over Select is $16.73 (USDA, 2015). Traditionally, USDA Select cuts are known to have lower palatability ratings for juiciness, tenderness, and overall liking. Select steaks also fail to meet consumer eating expectations more than 33% of the time (Corbin, 2015). This failure rate represents a large cost for the industry. Product enhancement utilizing a water, salt, and phosphate solution is commonly used in the pork and poultry industries to increase product eating satisfaction. This technology offers an opportunity for the beef industry to improve palatability as well. Previous research has shown enhancing beef results in a higher juiciness, tenderness, and overall liking ratings by consumers and trained panelists (Pietrasik and Janz, 2009). Previous research has shown enhancing Select cuts results in products that rate similar to Prime (Woolley, 2015). To date, it is unknown if enhancement of higher quality beef (Choice and Prime) results in the same increase in palatability observed in lower quality cuts. Therefore, the objective of this study was to determine the effect of enhancement on trained panel beef palatability scores of strip loins of three quality grades when cooked to three degrees of doneness