11 research outputs found
Pseudomonas aeruginosa Induced Airway Epithelial Injury Drives Fibroblast Activation:A Mechanism in Chronic Lung Allograft Dysfunction
Bacterial infections after lung transplantation cause airway epithelial injury and are associated with an increased risk of developing bronchiolitis obliterans syndrome. The damaged epithelium is a source of alarmins that activate the innate immune system, yet their ability to activate fibroblasts in the development of bronchiolitis obliterans syndrome has not been evaluated. Two epithelial alarmins were measured longitudinally in bronchoalveolar lavages from lung transplant recipients who developed bronchiolitis obliterans syndrome and were compared to stable controls. In addition, conditioned media from human airway epithelial cells infected with Pseudomonas aeruginosa was applied to lung fibroblasts and inflammatory responses were determined. Interleukinâ1 alpha (ILâ1α) was increased in bronchoalveolar lavage of lung transplant recipients growing P. aeruginosa (11.5 [5.4â21.8] vs. 2.8 [0.9â9.4] pg/mL, p < 0.01) and was significantly elevated within 3 months of developing bronchiolitis obliterans syndrome (8.3 [1.4â25.1] vs. 3.6 [0.6â17.1] pg/mL, p < 0.01), whereas high mobility group protein B1 remained unchanged. ILâ1α positively correlated with elevated bronchoalveolar lavage ILâ8 levels (r(2) = 0.6095, p < 0.0001) and neutrophil percentage (r(2) = 0.25, p = 0.01). Conditioned media from P. aeruginosa infected epithelial cells induced a potent proâinflammatory phenotype in fibroblasts via an ILâ1α/ILâ1Râdependent signaling pathway. In conclusion, we propose that ILâ1α may be a novel therapeutic target to limit Pseudomonas associated allograft injury after lung transplantation
IL-1α released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts
Epithelial-interleukin-1 inhibits collagen formation by airway fibroblasts: Implications for asthma
Conhecimento tĂĄtico-estratĂ©gico dos levantadores brasileiros campeĂ”es de voleibol: da formação ao alto nĂvel
Extracellular Vesicle-Associated Moonlighting Proteins: Heat Shock Proteins and Metalloproteinases
International Nosocomial Infection Control Consortium report, data summary of 50 countries for 2010-2015: Device-associated module
âąWe report INICC device-associated module data of 50 countries from 2010-2015.âąWe collected prospective data from 861,284 patients in 703 ICUs for 3,506,562 days.âąDA-HAI rates and bacterial resistance were higher in the INICC ICUs than in CDC-NHSN's.âąDevice utilization ratio in the INICC ICUs was similar to CDC-NHSN's.
Background: We report the results of International Nosocomial Infection Control Consortium (INICC) surveillance study from January 2010-December 2015 in 703 intensive care units (ICUs) in Latin America, Europe, Eastern Mediterranean, Southeast Asia, and Western Pacific.
Methods: During the 6-year study period, using Centers for Disease Control and Prevention National Healthcare Safety Network (CDC-NHSN) definitions for device-associated health care-associated infection (DA-HAI), we collected prospective data from 861,284 patients hospitalized in INICC hospital ICUs for an aggregate of 3,506,562 days.
Results: Although device use in INICC ICUs was similar to that reported from CDC-NHSN ICUs, DA-HAI rates were higher in the INICC ICUs: in the INICC medical-surgical ICUs, the pooled rate of central line-associated bloodstream infection, 4.1 per 1,000 central line-days, was nearly 5-fold higher than the 0.8 per 1,000 central line-days reported from comparable US ICUs, the overall rate of ventilator-associated pneumonia was also higher, 13.1 versus 0.9 per 1,000 ventilator-days, as was the rate of catheter-associated urinary tract infection, 5.07 versus 1.7 per 1,000 catheter-days. From blood cultures samples, frequencies of resistance of Pseudomonas isolates to amikacin (29.87% vs 10%) and to imipenem (44.3% vs 26.1%), and of Klebsiella pneumoniae isolates to ceftazidime (73.2% vs 28.8%) and to imipenem (43.27% vs 12.8%) were also higher in the INICC ICUs compared with CDC-NHSN ICUs.
Conclusions: Although DA-HAIs in INICC ICU patients continue to be higher than the rates reported in CDC-NSHN ICUs representing the developed world, we have observed a significant trend toward the reduction of DA-HAI rates in INICC ICUs as shown in each international report. It is INICC's main goal to continue facilitating education, training, and basic and cost-effective tools and resources, such as standardized forms and an online platform, to tackle this problem effectively and systematically