Microporous Biodegradable Films Promote Therapeutic Angiogenesis

Peripheral arterial disease and critical limb ischemia are common symptoms of cardiovascular disease. Vascular surgery is used to create a bypass around occluded blood vessels to improve blood flow to ischemic muscle, thus avoiding the need for amputation. Attempts to vascularize tissues by therapeutic angiogenesis using delivery of exogenous angiogenic agents are underwhelming. A material-based approach that provides an endogenous stimulus capable of promoting angiogenesis and increased tissue perfusion would provide a paradigm shift in treatment options available. It is reported here that microporous biodegradable films produced using thermally induced phase separation provide a localized biophysical stimulus of proangiogenic genes in vivo that is associated with increased blood vessel density and restoration of blood flow to ischemic tissue. These findings show, for the first time, that acellular, nonfunctionalized biodegradable biomaterials can provide an innovative, material-based approach for therapeutic angiogenesis to enhance tissue reperfusion in vivo

Pulse energy packing effects on material transport during laser processing of < 1

The effects of energy pulse packing on material transport during single-pulse laser processing of silicon is studied using temporarily shaped pulses with durations from 50 to 150 ns. Six regimes of material transport were identified and disambiguated through energy packing considerations over a range of pulse durations. Energy packing has been shown to shift the interaction to energetically costlier regimes without appreciable benefit in either depth, material removal or crater morphology and quality.The authors would like to thank the UK Technology Strategy Board under project TP14/HVM/6/I/BD5665. The authors acknowledge the EPSRC Centre for Doctoral Training in Photonic Systems Development for their generous support

Investigation of plume dynamics during picosecond laser ablation of H13 steel using high-speed digital holography

Ablation of H13 tool steel using pulse packets with repetition rates of 400 and 1000 kHz and pulse energies of 75 and 44μJ, respectively, is investigated. A drop in ablation efficiency (defined here as the depth per pulse or μm/μJ) is shown to occur when using pulse energies of E$_{pulse}$>44μJ, accompanied by a marked difference in crater morphology. A pulsed digital holographic system is applied to image the resulting plumes, showing a persistent plume in both cases. Holographic data are used to calculate the plume absorption and subsequently the fraction of pulse energy arriving at the surface after traversing the plume for different pulse arrival times. A significant proportion of the pulse energy is shown to be absorbed in the plume for E$_{pulse}$>44μJ for pulse arrival times corresponding to > 1 MHz pulse repetition rate, shifting the interaction to a vapour-dominated ablation regime, an energetically costlier ablation mechanism.This work was collaboratively carried out under EPSRC Grant Number EP/K030884/1, as part of the EPSRC Centre for Innovative Manufacturing in Laser-based Production Processes. One of the authors acknowledges his PhD studentship by the Federal Government of Nigeria (TETFUND) in conjunction with the Federal University of Petroleum Resources Effurun (FUPRE)

Formation of tribological structures by laser ablation

Many technical applications can benefit from the use of tribological structures in minimizing abrasive material wear and energy consumption without the integration of additional materials in a working assembly. Especially in lubricated friction systems, the tribological character can be significantly improved through the addition of oriented and repetitive microstructure. In this study, experimental tests are discussed for a small range of structure dimensions to verify the effect of optimizing the tribological contact performance. A nanosecond pulsed fiber laser is used to create various test structures with different sizes and form. The quality of the fabricated surface pattern, particularly form correctness, feathering and material modification effects of the ablated area is characteriz ed and optimized. The influence of pulse duration, pulse energy and pulse delay using normal pulsing is presented and compared to various burst modes

Ultrastructural Observations of the Vitelline Cells of Metamicrocotyla macracantha (Monogenea, Microcotylidae)

An electron microscopic study of the vitelline follicles of Metamicrocotyla macracantha (Alexander, 1954) Koratha,1955 showed that they are composed of cells in different stages of development. The immature cells have a large nucleus, nucleolus, cytoplasm with free ribosomes and few mitochondria. The developing vitelline cells present granules which are small in the early stages, increasing with maturity. The mature cells have an extensive granular endoplasmic reticulum and droplets of shell-protein; with maturation, clusters of shell protein and yolk bodies are formed and released in the ciliated vitelline ducts. Vitelline development is continuous and all of the cellular stages involved can be found in each follicle

Governing protracted displacement: an analysis across global, regional and domestic contexts

This working paper explores the governance of protracted displacement across global, regional and domestic levels in the context of the project “Transnational Figurations of Displacement” (TRAFIG). The multiple contemporary crises that have led to forced displacement show not only the limits of a tight definition of ‘refugee’, but also highlight the gaps in international protection frameworks. A significant number of those forcibly displaced are in protracted displacement situations. This paper is an effort to make sense of the legislative and policy frameworks of protection that apply globally, regionally and domestically, and the way in which these frameworks facilitate or hinder solutions for people in protracted displacement. We evaluate how these frameworks contribute (directly or indirectly) to resolving or creating protracted displacement, assess how they contribute to relevant policy developments and identify engagement trends and (unintended) effects. Along the way, we also draw comparative insights across different global, regional and domestic levels, including eight different countries that host large groups of displaced people and are the focus of the TRAFIG project: Greece, Germany and Italy in Europe; Ethiopia, the Democratic Republic of Congo (DRC) and Tanzania in Africa; and Jordan and Pakistan in Asia. We explore some selected gaps in the current systems of governance of displacement, while concentrating on three key perspectives: governing protection, exercising rights and accessing services, and mobility and transnational dimensions of displacement. We conclude with ten key messages regarding the shortcomings of the current governance system of displacement. They highlight the need for stronger stakeholder collaboration, integration of global and local policies, enhanced focus on IDPs, investment in progressive regional policies, redesign of EU policies to avoid promotion of protracted displacement, greater ownership of processes and resources, de-politicisation of displacement policies, alignment of durable solutions with development-oriented interventions, realisation of the development potential of refugee integration. They also focus on mobility and translocal connectivity as a fourth durable solution to protracted displacement