Evaluation of Hungarian Wines for Resveratrol by Overpressured Layer Chromatography

A method, including solid phase extraction sample preparation, overpressured layer chromatographic separation and subsequent densitometric evaluation, was developed for measurement of total resveratrol (cis- and trans-isomers) content of wine. The amount of resveratrol was determined in wine samples from different winemaking regions of Hungary. The total resveratrol was high in Hungarian red wines (3.6–11 mg/L), and much lower in white ones (0.04–1.5 mg/L)

Underweight and overweight men have greater exercise-induced dyspnoea than normal weight men.

INTRODUCTION: Persons with high or low body mass index (BMI), involved in clinical or mechanistic trials involving exercise testing, might estimate dyspnoea differently from persons with a normal BMI. AIMS: Our objective was to investigate the relationship between BMI and dyspnoea during exercise in normal subjects with varying BMI. MATERIAL AND METHODS: A total of 37 subjects undertook progressive exercise testing. Subjects were divided into three groups: underweight (UW), normal weight (NW), and overweight (OW). Dyspnoea was estimated using the visual analogue scale (VAS). Spirometry, maximum voluntary ventilation (MVV), and respiratory muscle strength (RMS) were measured. RESULTS AND DISCUSSION: The intercept of the VAS/ventilation relationship was significantly higher in NW subjects compared to UW (P = 0.029) and OW subjects (P = 0.040). Relative to the OW group, FVC (P = 0.020), FEV(1) (P = 0.024), MVV (P = 0.019), and RMS (P = 0.003) were significantly decreased in the UW group. The greater levels of dyspnoea in UW subjects could possibly be due to decreased RMS. Healthy persons should aim to achieve an optimum BMI range to have the lowest exercise-induced dyspnoea

Rescue of Photoreceptor Degeneration by Curcumin in Transgenic Rats with P23H Rhodopsin Mutation

The P23H mutation in the rhodopsin gene causes rhodopsin misfolding, altered trafficking and formation of insoluble aggregates leading to photoreceptor degeneration and autosomal dominant retinitis pigmentosa (RP). There are no effective therapies to treat this condition. Compounds that enhance dissociation of protein aggregates may be of value in developing new treatments for such diseases. Anti-protein aggregating activity of curcumin has been reported earlier. In this study we present that treatment of COS-7 cells expressing mutant rhodopsin with curcumin results in dissociation of mutant protein aggregates and decreases endoplasmic reticulum stress. Furthermore we demonstrate that administration of curcumin to P23H-rhodopsin transgenic rats improves retinal morphology, physiology, gene expression and localization of rhodopsin. Our findings indicate that supplementation of curcumin improves retinal structure and function in P23H-rhodopsin transgenic rats. This data also suggest that curcumin may serve as a potential therapeutic agent in treating RP due to the P23H rhodopsin mutation and perhaps other degenerative diseases caused by protein trafficking defects

Time Course of MERS-CoV Infection and Immunity in Dromedary Camels

Knowledge about immunity to Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels is essential for infection control and vaccination. A longitudinal study of 11 dam–calf pairs showed that calves lose maternal MERS-CoV antibodies 5–6 months postparturition and are left susceptible to infection, indicating a short window of opportunity for vaccination

The role of transdisciplinary approach and community participation in village scale groundwater management: insights from Gujarat and Rajasthan, India

Sustainable use of groundwater is becoming critical in India and requires effective participation from local communities along with technical, social, economic, policy and political inputs. Access to groundwater for farming communities is also an emotional and complex issue as their livelihood and survival depends on it. In this article, we report on transdisciplinary approaches to understanding the issues, challenges and options for improving sustainability of groundwater use in States of Gujarat and Rajasthan, India. In this project, called Managed Aquifer Recharge through Village level Intervention (MARVI), the research is focused on developing a suitable participatory approach and methodology with associated tools that will assist in improving supply and demand management of groundwater. The study was conducted in the Meghraj watershed in Aravalli district, Gujarat, and the Dharta watershed in Udaipur district, Rajasthan, India. The study involved the collection of hydrologic, agronomic and socio-economic data and engagement of local village and school communities through their role in groundwater monitoring, field trials, photovoice activities and education campaigns. The study revealed that availability of relevant and reliable data related to the various aspects of groundwater and developing trust and support between local communities, NGOs and government agencies are the key to moving towards a dialogue to decide on what to do to achieve sustainable use of groundwater. The analysis of long-term water table data indicated considerable fluctuation in groundwater levels from year to year or a net lowering of the water table, but the levels tend to recover during wet years. This provides hope that by improving management of recharge structures and groundwater pumping, we can assist in stabilizing the local water table. Our interventions through Bhujal Jankaars (BJs), (a Hindi word meaning “groundwater informed” volunteers), schools, photovoice workshops and newsletters have resulted in dialogue within the communities about the seriousness of the groundwater issue and ways to explore options for situation improvement. The BJs are now trained to understand how local recharge and discharge patterns are influenced by local rainfall patterns and pumping patterns and they are now becoming local champions of groundwater and an important link between farmers and project team. This study has further strengthened the belief that traditional research approaches to improve the groundwater situation are unlikely to be suitable for complex groundwater issues in the study areas. The experience from the study indicates that a transdisciplinary approach is likely to be more effective in enabling farmers, other village community members and NGOs to work together with researchers and government agencies to understand the groundwater situation and design interventions that are holistic and have wider ownership. Also, such an approach is expected to deliver longer-term sustainability of groundwater at a regional level

Participatory groundwater management at village level in India – empowering communities with science for effective decision making

There are many reasons behind the worsening groundwater situation that have led to a scarcity of quality water supply for sustaining lives and livelihoods in India, as well as in other parts of the world. The lack of a proper scientific understanding of this situation by the various stakeholders has been identified as one of the important gaps in the sustainable management of groundwater. This paper shares experiences from Gujarat and Rajasthan in western India where scientists, NGOs, government agencies and village leaders have worked together to explore strategies for sustainable groundwater management. The study involved a total of eleven villages in Gujarat and Rajasthan, India. The study’s main aim was to educate these communities through an intensive capacity building of (mainly) rural youth, called Bhujal Jaankars (BJs), a Hindi word meaning ‘groundwater informed’. The BJs were trained in their local settings through relevant theory and practical exercises, so that they could perform a geo-hydrological evaluation of their area, monitor groundwater and share their findings and experiences with their village community. The BJs went through a training program of a series of sessions totalling 45-days that covered mapping, land and water resource analysis, geo-hydrology, and water balance analysis, and finally groundwater management strategies. This approach has highlighted important learning that can be replicated in other parts of the two states and beyond. There are now 35 trained BJs who regularly monitor groundwater and rainfall in the two study watersheds, and provide data to both scientific and their own rural communities. This study has demonstrated that BJ capacity building has helped to provide a scientific basis for village level groundwater dialogue. This is now leading the communities and other stakeholders to improve their decision making regarding groundwater use, crop selection, agronomy, recharge strategies and other aspects of sustainable groundwater management. Although the BJ program has been successful and BJs can act as a valuable interface between local communities and other stakeholders, there still exists some challenges to the BJ programme, such as the need for mechanisms and funding sources that will sustain the BJs over the longer term; wider acceptance of BJs among scientific communities and policy makers; and the acceptance of the role and involvements of BJs in natural resources management programs of the State and Central governments in India