Retrospective Study on Milk Production and Reproductive Performance of Dairy Cattle in a Farm in Selangor, Malaysia

Dairy industry is small in Malaysia and unable to fulfill its dairy self-sufficiency. Therefore, Malaysia has to import most of the dairy products to satisfy its domestic demands. This study was performed to evaluate the performance of a selected dairy farm in Malaysia. A dairy farm in Selangor was selected and the farm records between 2011 and 2015 were analysed for the annual milk yield, calving rate and disease occurrence. It was found that the milk yield was low with average annual yield of 44,967 kg and the average milk production per cow per day was 6.83 kg. Clinical mastitis (67%) and traumatic injury (6.9%) were the most common disease occurrence. The calving rate of 75% was within the farm target. Calving percentage and diseases showed significant (P0.05) positive and negative influences on milk production, respectively

Anger mood detection based on face recognition and heartbeat

Anger is one of the feeling that showing strong resentment that can be found in facial expressions, body language, physiological responses and at times in public acts of aggression. So, this project has designed to examine the existence of the individual nature of anger using facial expression and heartbeat. The objective of this project is to warn and help people to control their temper. This can prevent the bad effect towards heart rate, blood pressure, levels of adrenaline and noradrenalin and other physical effects. Matlab-Image-processing is used to analyze facial expressions while heartbeat is measured using a heartbeat detector circuit. The Graphic User Interface (GUI) indicates the angry face result while the Liquid Crystal Display states the faster heartbeat when a person is angry

Passive maternal antibody transfer to eggs and larvae of tiger grouper (Epinephelus fuscoguttatus)

The immune response of Tiger grouper (E. fuscoguttatus) broodstocks and its passive transfer of maternal antibodies to eggs and larvae were evaluated following vaccination with an inactivated V.harveyi. Tiger grouper broodstock (mean BW 8.66 ± 0.09 kg, n=19) were vaccinated intraperitoneally (IP) and followed by a booster two weeks post vaccination, while Controlled Non-vaccinated (CG) broodstock were IP injected with PBS. The serum antibody level against V.harveyi was monitored for two weeks on post-vaccination and monthly up to 5 months post-vaccination. This study showed that the Vaccinated Group (VG) broodstock induced significantly (P<0.05) higher in specific IgM antibody level against V.harveyi as compared to the CG, which in turn induced a marked increased (P<0.05) in specific IgM in eggs and larvae produced from VG broodstock at 14 weeks post vaccination. The findings from this study suggested that inactivated V.harveyi vaccines were able to stimulate the immune response in broodstock and passively transferred the maternal antibody to their eggs and larvae

Geochemical distribution of heavy metals in peat soil profile and estimation of water table patterns in peatland at Klias Peninsular, Sabah

The geochemical distribution of heavy metals in peat soil profile and estimation of water table patterns of peatland in oil palm plantation were carried out in the Klias Peninsular West Coast of Sabah. The geological setting of the Klias Peninsular mainly consists of the Crocker Formation and Quaternary Alluvium which in mainly composed of peat soil. The peat soil from 10 sites were collected for the geochemical analysis. The geophysical surveys were performed to measure the water table, which influenced the concentration of trace metals. The geochemical analysis of the peat soil at 0-6m depth shows an acidic pH value (3.0-4.0), moisture content (90%- 1000%) and organic matter content (30-99) %. The texture of the peat soil profile varies from peat at the top of profile to silty clay at the bottom of the peat profile. The result of geochemical analysis shows that the element of Fe has the highest concentration (37.54-2049.18)mg/kg and followed by Zn(5.13-270.06)mg/kg, Mn(0.1-253.56)mg/kg, Pb(0.71-157.46)mg/kg, Cr(0.05- 152.62)mg/kg, Ni(0.1-64.48) mg/kg and Cu(1.81-61.39)mg/kg. The water table ranging from (0.25m-0.3m) induced the oxidation of Fe. The concentration of Fe2+ tends to absorb other elements such as Zn and Mn. Whereas, the organic matter at pH value (5-5.5) enhanced the absorption of elements Pb, Cr, Ni and Cu in the peat soil profile

Ultrastructural pathology of the upper respiratory tract of rabbits experimentally infected with Pasteurella multocida A:3

Twenty-four 8 to 9 week-old Pasteurella multocida-free rabbits were divided into three equal groups, the first group was pretreated with hydrocortisone and inoculated intranasally with pasteurella multocida serotype A:3. The second group was inoculated intranasally with P. multocida without hydrocortisone treatment. The third group was inoculated with phosphate buffered saline only and used as a control group. Pasteurella multocida was isolated from the nasal cavity of all infected rabbits in group 1 and 2 and from the trachea of seven rabbits in group 1 and five rabbits in group 2. This study was conducted to observe the ultrastructural changes of the upper respiratory tract of hydrocortisone treated and non-treated rabbits infected with P. multocida serotype A:3. The ultrastructural changes detected in infected rabbits were ciliary destruction and deciliation of the ciliated epithelial cells, cellular swelling, goblet cell hyperplasia and endothelial cell damage. Pasteurella multocida was observed attached to the degenerated cilia,microvilli and mucus. Pasteurella multocida infection was associated with inflammatory responses, which may have caused tissue damage. It is possible that hydrocortisone modulates the severity of infection as an immune suppressor and an inhibitor of goblet cell secretion

Microscopic Evaluation Of The Natural Coral (Porties spp.) Post-implantation In Sheep Femur.

Bone defects resulting from congenital defects, tumor or trauma are conventionally repaired using bone graft. Allogenic and xenogenic bone grafts are used as alternatives but several problems are generally associated with them such as virus transfer, considerable care, high cost and regular immuna-defensive reaction

Feed Formulation Based on Local Feed Resources and Its Effects on Nutritional-Related Blood Profile in Breeder Goats

A comparative study was conducted to determine the nutrient composition of different feed formulations and its effect on nutritional related blood profile in breeder goats. Eighteen female adult goats, Capra aegagrus hircus (Boer cross) were selected and allocated into three different groups (n = 6) and assigned according to different feed formulations. Blood sampling was conducted before and after fourth weeks of feeding trial and analysed for selected parameters. Proximate analysis of diets was done and revealed comparable value of crude protein and crude fat. Glucose, total protein and calcium level in serum showed significant difference between groups (P-value 0.05) while cholesterol and fatty acid showed no significant difference (P-value 0.05). Fatty acid analysis of feed and serum showed polyunsaturated fatty acid was higher than satureated fatty acid. Overall, diet 3 shows the highest value of all blood parameters analysed in this study. Hence, it is very important to provide animal with nutrient according to production stages as sufficent protein and energy will improve the animal performance in terms breeding efficacy and quality of animal products. This study indicates that different feed formulation affects certain nutritional related blood profile in goat

The Effect of Different Feed Formulation Based on Local Feed Resource on Performance and Stress Parameter in Breeder Goat

Balanced nutrition is essential for health and performance of livestock. It is best when feed is formulated according to production stages of goat. However, as the goat grows, it may undergo transition of different feed formulation to meet its daily requirement. The transition may affect the performance of animals as well as it is a stressful condition. In this study, body weight gain and feed intake were used as indicators on performance, whereas hematological stress leucograms were used as indicator of stress parameter during implementation of different feed formulation among does. Eighteen adult does with similar body condition score and body weight approximately 20 kg were selected and divided into three groups equally (n = 6) and fed with different feed formulations, which were formulated using local feed source in the farm for a period of four weeks. Body weight was measured before implementation, on week 2 and week 4, blood sampling was conducted before implementation and during week 4 for comparison and feed intake was measured every day. All data were statistically analyzed using SPSS. Does fed with Diet 3 showed highest body weight gain and feed intake, followed by Diet 2 and Diet 1. There was significant difference (P0.05) on body weight gain for all groups. Stress parameter on leucocyte counts for Diet 1 showed the highest number as compared to the other groups but no significant difference (P0.05) as compared to normal value. Neutrophils:lymphocytes ratio for all groups were within normal range and no significant difference (P0.05). The implementation of feed formulation according to production stages is recommended as it increases performance and prevents from nutritional stress

Comparison of Methods for Isolating High Quality DNA and RNA from an Oleaginous Fungus Cunninghamella bainieri Strain 2a1

A number of protocols have been reported for efficient fungal DNA and RNA isolation. However, many of these methods are often designed for certain groups or morphological forms of fungi and, in some cases, are species dependent. In this report, we compared four published protocols for DNA isolation from a locally isolated oleaginous fungus, Cunninghamella bainieri strain 2a1. These protocols either involved the use of polyvinyl pyrrolidone (PVP), hexacetyltrimethylammonium bromide (CTAB) or without using PVB or CTAB. For RNA isolation, we tested two published protocols, one of which is based on TRI REAGENT (Molecular Research Center, USA) and another is simple method employing phenol for RNA extraction and LiCl for precipitation. We found that the protocol involving the use of CTAB produced the highest genomic DNA yield with the best quality compared to other protocols. In the presence of CTAB, unwanted polysaccharides were removed and this method yielded an average amount of 816 ± 12.2 µg DNA/g mycelia with UV absorbance ratios A260/280 and A260/230 of 1.67 ± 0.64 and 1.97 ± 0.23, respectively. The genomic DNA isolated via this protocol is also suitable for PCR amplification and restriction enzyme digestion. As for RNA isolation, the method involving phenol extraction and LiCl precipitation produced the highest yield of RNA with an average amount of 372 ± 6.0 µg RNA/g mycelia. The RNA appears to be relatively pure since it has UV absorbance ratios A260/280 and A260/230 of 1.89 ± 2.00 and 1.99 ± 0.03, respectively. Finally, we have demonstrated that this method could produce RNA of sufficient quality for RT-PCR that amplified a 600 bp fragment of ∆12-fatty acid desaturase gene in C. bainieri

Water Thermocline Confirms Susceptibility of Tilapia Cultured in Lakes to Streptococcus agalactiae

A study was conducted on water quality profiling to confirm susceptibility of tilapia cultured in lakes to Streptococcus agalactiae infection. A total of 1,010 and 719 tilapias of different sizes were collected from two lakes; the Kenyir and Pedu lakes, respectively. They were randomly sampled for a period of 24 months. Swabs of brain, eye and kidney were streaked directly onto blood agar before S. agalactiae was identified by the API 20 STREP kit, Slidex Strepto-kit and PCR technique. The water temperature (thermocline) and dissolved oxygen profiling were determined at 1 m intervals for up to 20 m deep. Water clarity and flow rate were also recorded using Secchi disk and a current meter. S. agalactiae was successfully isolated from both lakes throughout the year, ranging between 2 and 78%. Isolation was more frequent during the hot and dry months of both years. During this period, the mean water temperature was >29 degrees C for up to 8 m deep due to the significantly (p12 m deep. This and the slow water flow kept the water temperature at 4 m deep where tilapias under the cage culture system were kept to remain high causing stress to tilapia and increases susceptibility to S. agalactiae. Dissolved oxygen profiling, however remained high at >5 mg L-1 for up to 8 m deep and did not give adverse effects to susceptibility of tilapia to S. agalactiae