8 research outputs found

    Biosorption of Arsenic(III) from Aqueous Solutions by Modified Fungal Biomass of Paecilomyces

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    The biosorption of As(III) on iron-coated fungal biomass of Paecilomyces sp. was studied in this work. It was found that the biomass was very efficient removing the metal in solution, using Atomic Absorption, reaching the next percentage of removals: 64.5%. The highest adsorption was obtained at pH 6.0, at 30°C after 24 hours of incubation, with 1 mg/L of modified fungal biomass

    Hexavalent Chromium (VI) Removal by Penicillium sp. IA-01

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    The objective of this work was to study the removal of chromium (VI) in aqueous solution by the fungus Penicillium sp. IA-01, isolated from polluted air with industrial vapors. To obtain the fungal biomass, pre-inoculums were performed in thioglycolate broth from a strain isolated from vapours contaminated with Cr (VI). The fungus was incubated for four weeks at ambient temperature, filtered, and washed three times with trideionized water. In preparing cellullar fractions, it was necessary to break the fungal cells with glass beads using a homogenizer being careful to keep the samples in frosty cold ice. To obtain the fungal biomass, the fungus was filtered and stored in an oven at 80°C, allowing it to dry for 48 h. Removal of Cr (VI) in vitro was evaluated using different cellular fractions and dead fungal biomass. We determine the optimal characteristics for metal removal in the reaction mixture. Concluding that the ideal conditions for the removal of Cr (VI) in the cell extracts were 37°C and pH 7.0, also we observ that the highest enzyme activity was in the mixed membrane fraction. In dead fungal biomass, the ideal conditions for removal of metal are 60°C and pH 1.0

    Remoción de cromo (VI) en solución por la cáscara de naranja (Citrus sinensis Osbeck)

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    The objective of this work was to determine the removal of Chromium (VI) by the orange peel biomass, by a spectrophotometric method using diphenylcarbazide as the complexing agent. Effects of time and temperature of incubation, pH and metal concentration were studied. The highest adsorption was obtained at pH= 1.0 ± 0.2, at 60°C after 10 minutes of incubation, with 50.0 g/L of initial concentration of Chromium (VI) and 1.0 g/100 mL of orange biomass, and remove fully 297 mg Cr (VI)/g of waste soil contaminated. It was concluded that the cellular biomass remove efficiently Cr (VI), and can be used for decontaminate wastewater.Se estudió la remoción de Cr (VI) en solución acuosa, mediante el empleo de biomasa obtenida de cáscara de naranja. Se observaron los efectos del tiempo y temperatura de incubación, el pH y la cantidad de metal sobre la remoción de éste. La concentración del metal en solución se determinó por la técnica de la difenilcarbazida. La mayor bioadsorción se obtuvo a los 10 minutos, a pH=1.0 ± 0.2, a 60ºC (50 mg/L de Cr VI) con 1 g de biomasa, y elimina totalmente 297 mg/g de tierra contaminada. La biomasa analizada remueve eficientemente el Cr (VI) en solución, por lo cual puede utilizarse para la eliminación de este metal en suelos contaminados y en aguas residuales

    ArtĂ­culo: RESISTANCE TO CRUDE OIL AND ALCOHOL OXIDASE ACTIVITY IN Mucor sp.

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    We isolated a fungal strain (Mucor sp), resistant to lead, the strain can grow in the presence of petroleum as the sole carbon source. Furthermore, the fungal strain shows good activity of alcohol oxidase in the cytosolic fraction with different substrates. It was concluded that this microorganism could be used for decontamination of aquatic habitats polluted with petroleum

    ArtĂ­culo: ISOLATION AND IDENTIFICATION OF BACTERIA AND FUNGI RESISTANT TO CRUDE OIL

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    Recently, has been studied the isolation of crude oil tolerant microorganisms and his capacity of degradation of crude oil in contaminated soils, such bacteria, yeast and fungi. Therefore, in this work, we isolate different microorganisms, which grow in presence of petroleum as the sole carbon source and we determined the activity of alcohol oxidase by a colorimetric method, in different cell fractions. Bacteria found were Serratia marcescens, Pseudomonas aeruginosa, Enterobacter aerogenes and Escherichia coli, the yeast, Candida albicans, and the fungi Aspergillus niger. In addition, they have good activity of alcohol oxidase in the cytosolic fraction with different substrates. It was concluded that this microorganisms could be used for decontamination of aquatic habitats polluted with petroleum

    ArtĂ­culo: BIOSORPTION OF CHROMIUM (VI) BY Citrus paradise SHELL

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    Cr (VI) is a toxic metal, which belongs to the list of priority pollutants due to its mutagenic and carcinogenic properties, defined by the US EPA. Contamination with Cr (VI) comes from electroplating, leather tanning, textile dyeing and metal finishing industries. Recently, a variety of low cost materials has been studied for their ability to remove this metal from aqueous solution with promising results. We studied the removal capacity of Chromium (VI) in solution by the grapefruit shell biomass, using the Diphenylcarbazide method to evaluate the metal concentration. Therefore, the highest biosorption of the metal (50 mg/L) occurs within 70 minutes, at pH of 1, and 28 °C. According to temperature, the highest removal was observed at 60°C, in 180 minutes, when the metal (1 g/L) is completely adsorbed. At the concentrations of Cr (VI) analyzed, the biomass showed excellent removal capacity, besides, removes efficiently the metal in situ (100% removal, 7 days of incubation, 5 g of biomass), and after of 1 hour of incubation the studied biomass reduces 1.0 g of Cr (VI) with the simultaneous production of Cr (III), so it can be used to eliminate it from industrial wastewater
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