ISOLATION, SCREENING AND CHARACTERIZATION OF L-ASPARAGINASE PRODUCING FUNGI FROM MEDICINAL PLANTS

Objective: To isolate and characterize of L-asparaginase producing fungi from medicinal plants.Methods: Fungal strains were isolated on standard media, characterized and were screened for their ability to produce L-asparginase, used in the treatment of certain types of cancers, using modified Czapek-Dox medium supplemented with L-asparagine.Results: A total of 10 fungal isolates were obtained from 3 plant leaf samples and two isolates exhibited significant L-aspargniase production. Fusarium sp. (SMGR-F1) isolated from the papaya leaves showed the maximum activity. The organism was grown under submerged fermentation conditions at 30 °C produced 111.07±1.53 IU/ml of L-asparaginase at 120 h.Conclusion: L-asparaginase is being effectively used for the treatment of acute lymphoblastic leukemia and tumor cells. Fusarium sp. was isolated from the papaya plant leaves showing significant L-asparaginase activity and thus can be further exploited for the commercial production of L-asparaginase.Â

Effect of shade materials on rectal temperature, respiration rate and body surface temperature of crossbred calves during rainy season

An attempt was made to study the effect of different shade materials on body surface temperature, rectal and respiration rate in Vrindavani calves during the rainy season. Crossbred calves (18) were divided into 3 group, viz. thatch shading roof with plastic covering (T1), agro-net shading roof- 60% light diffusion (T2), and asbestos with canvas shading roof (T3). The recording of macro and microclimate as well as the entire physiological parameters, viz. rectal temperature, respiration rate and body surface temperature were recorded at 9:00 and 2:00 PM for 2 consecutive days at every fortnight interval. The microclimate, viz. maximum and minimum, RH, THI and surface temperature of roof was lower in T2 group. The physiological parameters values were significantly lower in T2 group. It can be concluded that in rainy season agro-net helped to protect calves from the hot and humid condition

Effect of different heat ameliorating measures on the micro-climate of buffalo sheds during hot-dry summer

Present investigation studied the effect of heat ameliorating measures on micro-climate of sheds of Murrah buffalo heifers during hot dry summer. Buffalo heifers (24) were categorised in 4 groups (6 in each), viz. control (T0), cooling jacket (T1), cooling jacket+forced ventilation (T2) and sprinklers+forced ventilation (T3). Daily minimum and maximum and dry and wet bulb temperatures of both micro and macro-climate were measured at 10.00 AM and 2.00 PM of Indian Standard Time (IST) by using maximum and minimum and dry and wet bulb thermometer, respectively. Significant reduction in maximum temperature was observed in T3, T2 and T1 groups than control group. However, significantly higher minimum temperature was found in T0 group as compared to T3, T2 and T1 respectively. Relative humidity (RH) was significantly lower in T3 and T2 (65.67±0.48 and 65.97±0.53%, respectively) than T0 (68.51±0.56%) group at 10.00 AM whereas, at 2.00 PM significant reduction in RH was observed in T3, and T2 (55.31±0.51 and 57.20±0.62) than T0 (58.96±0.63%) group. During peak hot periods, significantly lower Temperature humidity Index (THI) was found in T3, T2 and T1 than T0 group. However, at 10.00 AM significant reduction in THI was found in T3 and T2 groups than T0 group. The findings of the present study indicated that forced ventilation along with sprinklers and cooling jacket had helped in protecting the animals from thermal stress under loose housing system during hot-dry summer

Effect of weaning on milking behaviour and temperament of Murrah buffaloes

The study was conducted to compare the milking behaviour and temperament of Murrah buffaloes due to suckling and weaning at different ages. Initially, 42 recently calved buffaloes were selected and assigned alternately in to 3 groups as suckling (Gr 1), weaning at birth (Gr 2) and weaning on 45th day (Gr 3). The different behavioural parameters of the buffaloes were recorded for the first 15 days of milking/weaning during each milking. The overall average milk let down time was non-significant among the groups. The average milk yield per milking (morning and evening) was significantly (P<0.01) higher in Gr 1 (3.44±0.14 kg) and Gr 2 (3.18±0.46 kg) in comparison to Gr 3 (2.24±0.25 kg). The overall average milking time was significantly (P<0.01) higher in Gr 1 (4.41±0.14 min) than Gr 3 (3.05±0.21 min); however milking time in Gr 2 (4.29±0.41min) was not significantly different from Gr 1. The percent of dams showing stepping behaviour in Gr 1, Gr 2 and Gr 3 was 20.23, 27.57 and 44.11, respectively, and the corresponding value for kicking behaviour was 2.61, 10.60 and 13.39, respectively. The average number of dams showing bellowing behaviour in Gr 1, Gr 2 and Gr 3 was 3.56, 9.09 and 12.66, respectively. Similar trends were observed among the groups as mentioned in stepping and kicking. The percentage of dams which licked calf or looked at milker in Gr 1, Gr 2 and Gr 3 was 34.99, 9.38 and 33.33 respectively. The percentage of dams showing head shaking activity during milking operation in Gr 1, Gr 2 and Gr 3 was 26.42, 0.00 and 10.00 percent, respectively. The overall mean milking temperament score was significantly (P<0.05) higher in Gr 3 (1.73±0.18) than Gr 1 (1.20±0.05) and Gr 2 (1.48±0.17). The success rate of weaning practice in dairy buffaloes was higher at calving than later stage of lactation

Proof-of-Principle Experiment for FEL-Based Coherent Electron Cooling,”

Abstract Coherent electron cooling (CEC) has a potential to significantly boost luminosity of high-energy, highintensity hadron-hadron and electron-hadron colliders. In a CEC system, a hadron beam interacts with a cooling electron beam. A perturbation of the electron density caused by ions is amplified and fed back to the ions to reduce the energy spread and the emittance of the ion beam. To demonstrate the feasibility of CEC we propose a proof-of-principle experiment at RHIC using SRF linac. In this paper, we describe the setup for CeC installed into one of RHIC&apos;s interaction regions. We present results of analytical estimates and results of initial simulations of cooling a gold-ion beam at 40 GeV/u energy via CeC

Efficacy of an electrically active sonic toothbrush and an oscillating/rotating powered toothbrush in the reduction of plaque and gingivitis: A comparative clinical trial

Objectives: The purpose of the present study was to clinically evaluate and compare the efficacy of an electrically active sonic toothbrush and an oscillating/rotating powered toothbrush in the reduction of plaque and gingivitis. Material and Methods: For this study, 40 healthy student volunteers (20 males, 20 females) were selected. The subjects were randomly assigned into two groups by a second examiner; one group used a current producing sonic toothbrush and the other group used a battery powered toothbrush. Plaque Index, Modified Gingival Index and Gingival Bleeding Index were assessed at baseline, 15 th day, 30 th day, 45 th day and 60 th day. Results: All the baseline indices appeared to be well balanced. At the end of the study, reduction in plaque index, modified gingival index and gingival bleeding index were statistically highly significant during each interval for both the toothbrushes. The difference in reduction of clinical parameters between the two toothbrushes was statistically non significant. Conclusion: Both the toothbrushes used in this study were clinically effective in removing plaque and improving gingival health

Role of platelet-rich-fibrin in enhancing palatal wound healing after free graft

Platelet-rich fibrin has long been used as a wound healing therapy in skin wounds and recently evidence has suggested its usage in oral cavity for different treatment procedures. This article proposes an overview of use of platelet-rich fibrin in management of complicated oral wounds. Excessive hemorrhage of the donor area, necrosis of epithelium, and morbidity associated with donor site have been described as the possible complications after harvesting subepithelial connective tissue graft, but little has been mentioned about their management. The article includes a case report of a 45-year-old male patient who showed a delayed wound healing after subepithelial connective tissue graft harvestation, which was treated with platelet-rich fibrin

Growth Dynamics and Yield of Zero-Till Maize as Influenced by Microbial Consortia Treated Paddy Residue Incorporation

A field experiment was conducted at College farm, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad during 2020-21 and 2021-22 to assess the influence of paddy residue management practices at different fertility levels on certain growth parameters and the yield of rabi maize succeeding kharif rice in a strip plot design. The growth analysis indicated that the leaf area index, crop growth rate, net assimilation rate and leaf area duration remained the same with residue management practices at 0-30 DAS and 30-60 DAS but between 60 DAS-harvest, maize leaf area index (LAI), crop growth rate (CGR), net assimilation rate (NAR) and leaf area duration (LAD) was significantly increased by incorporation of crop residues treated with microbial consortia and SSP. Different fertility levels affected leaf area index, crop growth rate and leaf area duration at all the growth intervals whereas net assimilation rate remained unaffected. Interaction effect of residue management practices and fertility levels on leaf area index, crop growth rate, net assimilation rate and leaf area duration was found non significant at 0-30 DAS and 30-60 DAS, while at 60 DAS-harvest integration of residues and microbial consortia along with chemical fertilizers resulted in a significant increase of LAI, CGR and LAD with no significant improvement in NAR. In 2020-21 and 2021-22, the grain yield and straw yield were higher in the incorporation of crop residues treated with microbial consortia and single super phosphate (SSP) in combination with 125 % recommended dose of fertilizers (RDF) which was on par with 100% RDF and 75 % RDF and was significantly superior over other treatments

Successful management of phenytoin and phenobarbitone induced gingival enlargement: A multimodal approach

Medication-related gingival enlargement is a common reactionary phenomenon that occurs with the use of several types of therapeutic agents, including antiepileptic drugs (AEDs). This disorder has been documented since 1939, shortly after the introduction of phenytoin. In the present case, a concise review of literature concerning the etiopathogenesis and management of AEDs (phenobarbitone and phenytoin) induced gingival enlargement has been described. It is vital that not only the periodontist, but also dental surgeons and medical practitioners should become aware of the potential etiologic agents, characteristic features, and the differential diagnosis of drug induced gingival enlargement in order to be able to prevent, diagnose and successfully manage the condition

Design and Implementation of Wireless Sensor Network for Environmental Monitoring

Wireless Sensor Networks (WSN) are being developed in the agriculture and weather station sectors to monitor and manage a number of objects. Sensor network hardware platforms are low-power embedded systems featuring a variety of sensors, such as on-board sensors and analogue I/O ports for adding sensors. The design of genuine environmental monitoring and communication systems is greatly aided by the creation of a WSN. A WSN is a collection of sensors that are geographically spread and specialized for monitoring and recording environmental variables and storing the findings in a central location. Limited power consumption as sensor nodes works on batteries, Nodes are mobile opposed to conventional wires, Usage in harsh environment, Nodes can be deployed in any Fashion over an area, Easy to use and multi-layer design operation these are the main characteristics of WSN. Limits imposed on the energy storage device by their applications and deployment settings are less relevant to WSN environment-monitoring applications than controlling power consumption and optimizing data transmission. A WSN is used to record real-time traffic and environmental data in order to develop descriptive and predictive models for determining the optimum routes to reduce traffic congestion and, as a result, urban pollution.