A Balint curriculum's effect on family medicine resident empathy and psychological skills

Balint groups are comprised of physicians who regularly gather to present and reflect on clinical cases with the goal of improving the physician-patient relationship by enhancing the physician's interpersonal and communication skills, patient care, and professionalism. The goal of this study was to assess the effect on empathy and psychological skills of a voluntary Balint curriculum in the University of Missouri Family Medicine Residency program since initiation in 2010

Statistical processing and visualization of the medical data

The aim of the research is to consider basic concepts of descriptive statistics and to show basic histograms for the medical data

Serial intravital 2-photon microscopy and analysis of the kidney using upright microscopes

Serial intravital 2-photon microscopy of the kidney and other abdominal organs is a powerful technique to assess tissue function and structure simultaneously and over time. Thus, serial intravital microscopy can capture dynamic tissue changes during health and disease and holds great potential to characterize (patho-) physiological processes with subcellular resolution. However, successful image acquisition and analysis require significant expertise and impose multiple potential challenges. Abdominal organs are rhythmically displaced by breathing movements which hamper high-resolution imaging. Traditionally, kidney intravital imaging is performed on inverted microscopes where breathing movements are partly compensated by the weight of the animal pressing down. Here, we present a custom and easy-to-implement setup for intravital imaging of the kidney and other abdominal organs on upright microscopes. Furthermore, we provide image processing protocols and a new plugin for the free image analysis software FIJI to process multichannel fluorescence microscopy data. The proposed image processing pipelines cover multiple image denoising algorithms, sample drift correction using 2D registration, and alignment of serial imaging data collected over several weeks using landmark-based 3D registration. The provided tools aim to lower the barrier of entry to intravital microscopy of the kidney and are readily applicable by biomedical practitioners