Crispr gene editing in lipid disorders and atherosclerosis: Mechanisms and opportunities

Elevated circulating concentrations of low-density lipoprotein cholesterol (LDL-C) have been conclusively demonstrated in epidemiological and intervention studies to be causally associated with the development of atherosclerotic cardiovascular disease. Enormous advances in LDL-C reduction have been achieved through the use of statins, and in recent years, through drugs targeting proprotein convertase subtilisin/kexin type 9 (PCSK9), a key regulator of the hepatic LDL-receptor. Existing approaches to PCSK9 targeting have used monoclonal antibodies or RNA interference. Although these approaches do not require daily dosing, as statins do, repeated subcutaneous injections are nevertheless necessary to maintain effectiveness over time. Recent experimental studies suggest that clustered regularly interspaced short palindromic repeats (CRISPR) gene-editing targeted at PCSK9 may represent a promising tool to achieve the elusive goal of a ‘fire and forget’ lifelong approach to LDL-C reduction. This paper will provide an overview of CRISPR technology, with a particular focus on recent studies with relevance to its potential use in atherosclerotic cardiovascular disease

Large-Scale Statistical Analysis of Defect Emission in hBN: Revealing Spectral Families and Influence of Flakes Morphology

Quantum emitters in two-dimensional layered hexagonal boron nitride are quickly emerging as a highly promising platform for next-generation quantum technologies. However, precise identification and control of defects are key parameters to achieve the next step in their development. We conducted a comprehensive study by analyzing over 10,000 photoluminescence emission lines, revealing 11 distinct defect families within the 1.6 to 2.2 eV energy range. This challenges hypotheses of a random energy distribution. We also reported averaged defect parameters, including emission linewidths, spatial density, phonon side bands, and the Debye-Waller factors. These findings provide valuable insights to decipher the microscopic origin of emitters in hBN hosts. We also explored the influence of hBN host morphology on defect family formation, demonstrating its crucial impact. By tuning flake size and arrangement we achieve selective control of defect types while maintaining high spatial density. This offers a scalable approach to defect emission control, diverging from costly engineering methods. It highlights the importance of investigating flake morphological control to gain deeper insights into the origins of defects and to expand the spectral tailoring capabilities of defects in hBN

Welfare and performance traits of meat rabbit females. A review

[ES] El manejo de reproductoras en grupo o semigrupo, con objeto de mejorar su bienestar, proporciona solo un ligero enriquecimiento del comportamiento locomotor y social, y algunos resultados zootécnicos próximos pero estadísticamente inferiores a los obtenidos en jaula individual, sin conseguir así compensar los resultados negativos del método: mayor mortalidad de gazapos lactantes, estrés, agresividad, heridas y mortalidad de las madres, unido a la complejidad, tiempo y trabajo requeridos por este manejo. Enriquecer la jaula individual mediante plataforma no deteriora los resultados zootécnicos, permite incrementar la movilidad y el repertorio comportamental de las conejas (saltar, erguirse), aumentando además la superficie disponible, pero empeora la higiene y aumentan los problemas sanitarios (pododermatitis, fracturas). Reposapatas y elementos para roer, así como refinar el enriquecimiento en las jaulas estándar, parece una vía útil para mejorar el bienestar mientras se profundiza en la gestión de la respuesta agresiva, así como en la idoneidad y en el modelo de grupo en las reproductoras de carne.[EN] Continuous and part-time group housing systems have been tested to improve the welfare of reproducing rabbit does. Part-time group provides some improvements in locomotor and social behavior, some good reproductive results ¿still significantly lower than individual cages¿, without making up for its negative effects: higher kit mortality, rates of aggression, injured and does mortality, on top of the complexity, time and effort invested. The continuous group system increases more the aforementioned problems. Individual cages enriched by a platform have no negative effect on productive performances, provide more opportunities for movements and behavioral repertoire in does (jumping, stand up), increase space availability, but deteriorate hygiene and health (pododermatitis, fractures). Plastic footrests, gnawing blocks, have a positive impact on improving welfare in rabbits. In conclusion, refining the enrichment of standard cages seems a useful way to improve welfare while studying how to reduce aggression, as well as the suitability of the breeding group and the group model in the meat rabbit does.López, M.; Cervera Fras, MC.; Pascual Amorós, JJ. (2020). Bienestar y resultados zootécnicos en conejas de aptitud carne. Revisión bibliográfica. ITEA-Informacion Tecnica Economica Agraria. 116(2):131-149. https://doi.org/10.12706/itea.2020.002S131149116

Bio-inspired octopus robot based on novel soft fluidic actuator

This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.European Commission’s project Horizon 2020 Research and Innovation Programme, project FourByThree under grant agreement No 63709

Effect of increasing lignin in isoenergetic diets at two soluble fibre levels on digestion, performance and carcass quality of growing rabbits

[EN] To assess the effect of increasing dietary lignin in isoenergetic diets at two soluble fibre (SF) levels on digestion, performance and carcass quality of growing rabbits, four diets were formulated according a 2 x 2 factorial design: low SF-low lignin (LSF/LL), low SF-high lignin (LSF/HL), high SF-low lignin (HSF/LL) and high SF-high lignin (HSF/HL). On average, in HSF diets SF was increased by 49 g/kg DM, mainly replacing starch ( - 53 g/kg DM), and in HL diets lignin was increased by 40 g/kg, mainly reducing starch (-78 g/kg DM), with increasing EE ( + 31 g/kg DM). Two hundred and sixty crossbred weaned rabbits (35 days old) were assigned to the experimental diets, individually housed and fed ad libitum until 63 days of age. Digestibility (from 49 to 53 days old), growth performance (from 35 to 63 days old), carcass quality (at 63 days old) and caecal environment (at 63 days old) were studied in 12, 65, 45 and 16 rabbits per diet, respectively. High SF diets showed higher CTTAD of fibrous fractions ( + 0.206 +/- 0.011, + 0.207 +/- 0.015, + 0.214 +/- 0.011 and + 0.167 +/- 0.015 for aNDFom, ADFom, hemicelluloses and cellulose, respectively, P < 0.001), OM ( + 0.042 +/- 0.004, P < 0.001) and GE ( + 0.055 +/- 0.005, P < 0.001), resulting in high DE content (10.6 vs. 9.30 MJ/kg DM). In contrast, CTTAD of CP was lower ( - 0.023 +/- 0.009, P = 0.013), as well as the DP content (96.9 vs. 103 g/kg DM). This dietary variation reduced the DM content of caecal digesta ( - 28 +/- 3 g/kg, P < 0.001), besides increasing its VFA concentration ( + 18.0 +/- 4.0 mmol/L, P < 0.001) and reducing its pH ( - 0.28 +/- 0.05, P < 0.001). Feed intake and LW gain decreased, with an improvement of feed to gain ratio ( -13.8 %, - 4.7 %, - 9.4 %, respectively; P < 0.001). The proportion of gastrointestinal tract was increased, with a subsequent reduction in dressing out ( + 19 +/- 2 g/kg LW and -15 +/- 2 g chilled carcass weight/kg LW, respectively, P < 0.001). High lignin diets showed lower CTTAD of OM (-0.055 +/- 0.004, P < 0.001) and GE ( - 0.034 +/- 0.005, P < 0.001) without affecting DE and DP contents. This dietary variation increased DM content of caecal digesta ( +21 +/- 3 g/kg, P < 0.001), but did not affect the other caecal digesta traits. Feed intake was higher ( + 4.9 %, P < 0.001), although differences were dependent on the growth phase and the SF level (maximum difference at 35-49 days with low SF diets, + 11.0 %, P < 0.001; minimum difference at 49-63 days with high SF diets, + 1.0 %, P = 0.689), but did not affect LW gain and consequently impaired the feed to gain ratio ( + 5.1 %, P < 0.001). No effect was observed on dressing out, but the dissectible fat proportion increased ( + 6.7 +/- 1.1 g/kg reference carcass weight, P < 0.001).Financial support was provided by the Spanish Ministry of Education and Science (Project AGL2006-07596).Caisin, L.; Martinez-Paredes, E.; Ródenas Martínez, L.; Moya, V.; Pascual Amorós, JJ.; Cervera Fras, MC.; Blas Ferrer, E.... (2020). Effect of increasing lignin in isoenergetic diets at two soluble fibre levels on digestion, performance and carcass quality of growing rabbits. Animal Feed Science and Technology. 262:1-9. https://doi.org/10.1016/j.anifeedsci.2020.114396S19262Batey, I. L. (1982). Starch Analysis Using Thermostable alpha-Amylases. Starch - Stärke, 34(4), 125-128. doi:10.1002/star.19820340407Delgado, R., Nicodemus, N., Abad-Guamán, R., Sastre, J., Menoyo, D., Carabaño, R., & García, J. (2018). Effect of dietary soluble fibre and n-6/n-3 fatty acid ratio on growth performance and nitrogen and energy retention efficiency in growing rabbits. Animal Feed Science and Technology, 239, 44-54. doi:10.1016/j.anifeedsci.2018.03.006Delgado, R., Menoyo, D., Abad-Guamán, R., Nicodemus, N., Carabaño, R., & García, J. (2019). Effect of dietary soluble fibre level and n-6/n-3 fatty acid ratio on digestion and health in growing rabbits. Animal Feed Science and Technology, 255, 114222. doi:10.1016/j.anifeedsci.2019.114222Fernández, C., & Fraga, M. J. (1996). The effect of dietary fat inclusion on growth, carcass characteristics, and chemical composition of rabbits. Journal of Animal Science, 74(9), 2088. doi:10.2527/1996.7492088xGarcía, J., Gidenne, T., Luisa Falcao-e-Cunha, & de Blas, C. (2002). Identification of the main factors that influence caecal fermentation traits in growing rabbits. Animal Research, 51(2), 165-173. doi:10.1051/animres:2002011Gidenne, T. (2015). Dietary fibres in the nutrition of the growing rabbit and recommendations to preserve digestive health: a review. Animal, 9(2), 227-242. doi:10.1017/s1751731114002729Gidenne, T., Perez, J., Lapanouse, A., & Ségura, M. (1994). Apports de lignines et alimentation du lapin en croissance. I. Conséquences sur la digestion et le transit. Annales de Zootechnie, 43(4), 313-322. doi:10.1051/animres:19940401Gidenne, T., Arveux, P., & Madec, O. (2001). The effect of the quality of dietary lignocellulose on digestion, zootechnical performance and health of the growing rabbit. Animal Science, 73(1), 97-104. doi:10.1017/s1357729800058094Grueso, I., De Blas, J. C., Cachaldora, P., Mendez, J., Losada, B., & García-Rebollar, P. (2013). Combined effects of supplementation of diets with hops and of a substitution of starch with soluble fiber on feed efficiency and prevention of digestive disorders in rabbits. Animal Feed Science and Technology, 180(1-4), 92-100. doi:10.1016/j.anifeedsci.2013.01.009Martínez-Vallespín, B., Martínez-Paredes, E., Ródenas, L., Cervera, C., Pascual, J. J., & Blas, E. (2011). Combined feeding of rabbit female and young: Partial replacement of starch with acid detergent fibre or/and neutral detergent soluble fibre at two protein levels. Livestock Science, 141(2-3), 155-165. doi:10.1016/j.livsci.2011.05.014Martínez-Vallespín, B., Martínez-Paredes, E., Ródenas, L., Moya, V. J., Cervera, C., Pascual, J. J., & Blas, E. (2013). Partial replacement of starch with acid detergent fibre and/or neutral detergent soluble fibre at two protein levels: Effects on ileal apparent digestibility and caecal environment of growing rabbits. Livestock Science, 154(1-3), 123-130. doi:10.1016/j.livsci.2013.02.012Nicodemus, N., Carabaño, R., Garcı́a, J., Méndez, J., & de Blas, C. (1999). Performance response of lactating and growing rabbits to dietary lignin content. Animal Feed Science and Technology, 80(1), 43-54. doi:10.1016/s0377-8401(99)00042-5Ocasio-Vega, C., Delgado, R., Abad-Guamán, R., Carabaño, R., Carro, M. D., Menoyo, D., & García, J. (2018). The effect of cellobiose on the health status of growing rabbits depends on the dietary level of soluble fiber. Journal of Animal Science, 96(5), 1806-1817. doi:10.1093/jas/sky106Pascual, M., Soler, M. D., Cervera, C., Pla, M., Pascual, J. J., & Blas, E. (2014). Feeding programmes based on highly-digestible fibre weaning diets: Effects on health, growth performance and carcass and meat quality in rabbits. Livestock Science, 169, 88-95. doi:10.1016/j.livsci.2014.07.007Trocino, A., García Alonso, J., Carabaño, R., & Xiccato, G. (2013). A meta-analysis on the role of soluble fibre in diets for growing rabbits. World Rabbit Science, 21(1). doi:10.4995/wrs.2013.1285Trocino, A., Fragkiadakis, M., Majolini, D., Tazzoli, M., Radaelli, G., & Xiccato, G. (2013). Soluble fibre, starch and protein level in diets for growing rabbits: Effects on digestive efficiency and productive traits. Animal Feed Science and Technology, 180(1-4), 73-82. doi:10.1016/j.anifeedsci.2013.01.007Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74(10), 3583-3597. doi:10.3168/jds.s0022-0302(91)78551-2Xiccato, G., Trocino, A., Majolini, D., Fragkiadakis, M., & Tazzoli, M. (2011). Effect of decreasing dietary protein level and replacing starch with soluble fibre on digestive physiology and performance of growing rabbits. Animal, 5(8), 1179-1187. doi:10.1017/s175173111100024

Analysis of public food procurement in relation to dairy products and their quality criteria

IntroductionPublic procurement of food is key to ensuring proper nutrition with high-quality products in public institutions such as schools and kindergartens. However, it should not be considered a mandatory expenditure from public finances but rather an investment in health promotion.Materials and methodsA total of 1,126 public procurement orders processed by schools and kindergartens in Poland during the period from November 2022 to March 2023 were analyzed. Ultimately, 197 public procurement orders meeting the inclusion criteria were considered for analysis. Based on these orders, 2,753 food products classified as dairy and its derivatives were extracted. The ordered quantities of individual products were analyzed, as well as their descriptions (quality characteristics).ResultsCriteria related to composition were most commonly described, the most common criterion was the fat content and the absence of preservatives. On the second places were organoleptic characteristics, where taste and consistency expectations were most frequently specified. Sustainable public procurement criteria were the least frequently mentioned and were treated as highly marginal.ConclusionIntroducing minimum standards for the descriptions of dairy products in terms of organoleptic characteristics, composition features, and sustainability criteria will improve the quality of dairy products supplied to public institutions, particularly schools and kindergartens

Spatial patterns of dissipative polariton solitons in semiconductor microcavities

We report propagating bound microcavity polariton soliton arrays consisting of multipeak structures either along (x) or perpendicular (y) to the direction of propagation. Soliton arrays of up to five solitons are observed, with the number of solitons controlled by the size and power of the triggering laser pulse. The breakup along the x direction occurs when the effective area of the trigger pulse exceeds the characteristic soliton size determined by polariton-polariton interactions. Narrowing of soliton emission in energymomentum space indicates phase locking between adjacent solitons, consistent with numerical modeling which predicts stable multihump soliton solutions. In the y direction, the breakup originates from inhomogeneity across the wave front in the transverse direction which develops into a stable array only in the solitonic regime via phase-dependent interactions of propagating fronts