Revealing Our Melting Past: Rescuing Historical Snow and Ice Data

Analog archival data can supplement modern digital research, but only if those data are preserved, described, and migrated to appropriate formats. The National Snow and Ice Data Center (NSIDC) at the University of Colorado Boulder (CU) is responsible for managing, archiving, and disseminating cryospheric and polar data. The clear majority of these data are digital, but the NSIDC also houses a collection of historical archival materials that include measurements related to the earth\u27s glaciated regions prior to the development of modern instrumentation. Their formats, however, are not conducive to contemporary analysis, rendering them ostensibly “lost” to research. This paper describes a series of efforts to provide access to these collections that date back to their original acquisition, as long ago as the mid-nineteenth century, with focus primarily on activities over the last 15 years. The most recent effort was funded by the Council on Library & Information Resources and won the 2016 International Data Rescue Award. The intent is to highlight key challenges, and our proposed own solutions to those challenges, in designing a digitization project centered on providing online access to analog data in glaciological, geomorphological, and related research

Characterization of a nicotinamide-adenine dinucleotide-dependent cation channel in the CRI-G1 rat insulinoma cell line.

1. Cell-free excised membrane patches were used to examine the properties of a novel nicotinamide-adenine dinucleotide (beta-NAD+)-activated ion channel in the rat insulin-secreting cell line, CRI-G1. 2. In inside-out recordings, beta-NAD+ (0.05-1.0 mM) induced the appearance of a channel characterized by extremely slow kinetics, with mean open times in the range of seconds. The estimated EC50 for activation was 114 microM. Channel activity declined with time (run-down) following activation by beta-NAD+ in excised patches and this was not prevented by intracellular application of trypsin. 3. The single channel current-voltage relationship was linear with a conductance of 74 pS in symmetrical NaCl. The channel appears equally permeable to Na+, K+ and Cs+, exhibits an appreciable permeability to Ca2+, Mg2+ and Ba2+, but excludes anions. 4. The channel displays an unusual voltage sensitivity, with an abrupt increase in open-state probability at depolarized voltages. 5. Channel opening, in the presence of beta-NAD+, required both Ca2+ and Mg2+ to be present at the internal side of the membrane. Activation by Ca2+ required a concentration of at least 10 microM and was maximal at 0.1 mM. Ba2+ did not substitute for Ca2+ in inducing channel activity nor did it inhibit activation by Ca2+. Increasing the concentration of intracellular Mg2+ stabilized the open state of NAD(+)-activated channels. 6. The non-selective cation channel reported here differs in its gating and modulatory characteristics from non-selective cation channels described in other tissues. This channel may play a role in the pathophysiological responses of beta-cells to oxidative stress