1,190 research outputs found
Identification of a novel type of spacer element required for imprinting in fission yeast
Asymmetrical segregation of differentiated sister chromatids is thought to be important for cellular differentiation in higher
eukaryotes. Similarly, in fission yeast, cellular differentiation involves the asymmetrical segregation of a chromosomal
imprint. This imprint has been shown to consist of two ribonucleotides that are incorporated into the DNA during laggingstrand
synthesis in response to a replication pause, but the underlying mechanism remains unknown. Here we present key
novel discoveries important for unravelling this process. Our data show that cis-acting sequences within the mat1 cassette
mediate pausing of replication forks at the proximity of the imprinting site, and the results suggest that this pause dictates
specific priming at the position of imprinting in a sequence-independent manner. Also, we identify a novel type of cis-acting
spacer region important for the imprinting process that affects where subsequent primers are put down after the
replication fork is released from the pause. Thus, our data suggest that the imprint is formed by ligation of a not-fullyprocessed
Okazaki fragment to the subsequent fragment. The presented work addresses how differentiated sister
chromatids are established during DNA replication through the involvement of replication barriers
Concepts for a multi-criteria sustainability assessment of a new more biobased economy in rural production landscapes
This study presents a new approach to model the biophysical potentials for increased biomass harvest in Denmark, and a multi-criteria sustainability assessment of the socio-economic and environmental effects of different scenarios for such increased production and new biobased economy in rural landscapes. Thereby the results serve as input to the ongoing productivism – post-productivism debate related to rural spaces in Europe.
Empirical results from a case study of large scale conversion to biorefenery technologies, aiming to increase the total Danish harvest of biomass by 10 million tonnes via the conversion to new types of crop production and land management, is used as an example, and will be related to the common conceptual model presented by the workshop convenors, and a so called “Traffic-light” multi-criteria sustainability assessment scheme introduced and exemplified in the present paper. The aim is to contextualize how the problems in relation to the concrete scenario results can be considered with the landscape as the meeting basis, and used as input for a discussion of innovative models for future farming systems, landscape research and management
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