In-farm cost of an outbreak of diarrhoea in lambs

This article analyses the cost of diarrhoea in lambs on dairy sheep farms located in Grosseto (Italy). Farmers’ profits have recently declined due to a stable product price but increasing production costs. Animal diseases have a cascade of effects on farm productivity. Lamb enteric disease outbreaks, which result in mortality in the herd and reduced weight gain, can drastically compromise the income of farmers. An economic analysis of the impact of an outbreak of diarrhoea in lambs was thus performed, evaluating the cost of disease based on the main visible production losses (such as mortality, reduced weight gain and variation in milk production). A sensitivity analysis was also conducted by applying different observed ranges of prevalence and mortality associated with the disease. Finally, an economic scenario analysis was performed, considering different in-farm management options for delivering lambs to the abattoir, i.e. early, standard and late delivery. The results showed that a dairy sheep farm with around 300 lambs that delivers them to the abattoir at 30 days of age would experience a loss of between 50 and 1200 Euro during an outbreak of diarrhoea with a prevalence of 34.21 (23.54–44.88)% and a mortality of 15.69 (9.98–21.4)%

Mouthwash based on ozonated olive oil in caries prevention: a preliminary in-vitro study

(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against Streptococcus mutans. (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose (IR) (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2-to 256-folds in saline solution, as to reproduce the salivary dilution. Streptococcus mutans CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby–Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate Streptococcus mutans avoiding the salivary dilution effect in the oral cavity

Multimodal Surveillance of HAI in an Intensive Care Unit of a Large Teaching Hospital

Background: Healthcare associated Infections (HAIs) represent a significant burden in terms of mortality, morbidity, length of stay and costs for patients in intensive care units (ICU). Surveillance systems are recommended to gather data in order to elaborate and evaluate intervention to reduce HAIs risk. Here we describe results of the multimodal surveillance system implemented in the ICU of a large teaching hospital in Rome from April 2016 to October 2018. Methods: The surveillance system integrated four different approaches: i) active surveillance focused on inpatients; ii) environmental microbiological surveillance; iii) surveillance focused on isolated microorganisms; iv) behavioral surveillance of the healthcare personnel. The system included the molecular genotyping of bacterial isolates through the pulsed field gel electrophoresis (PFGE). Moreover, an intervention to improve personnel adherence to hand hygiene (HH) guidelines was conducted. Results: Overall, 773 patients were included in the surveillance. The global incidence rate of the device related HAIs was 14.1 (95%CI: 12.2-16.3) per 1000 patient day. Monthly device related HAIs incidence rate showed a decreasing, from 26.9 per 1000 patient day in October 2016, to 4.9 in September 2018. The most common bacterial isolate was K. pneumoniae (20.7%), the 94.0% of which were multidrug-resistant. A total of 305 environmental bacterial isolates were retrieved and the most frequent was A. baumannii (27.2%), that was always multidrug-resistant. Genotyping showed a limited number of major PFGE patters in clinical and environmental isolates. Behavioral compliance to HH guidelines improved after the educational intervention. Conclusions: The data showed an overall slight decrease over time of the adjusted risk HAIs rates. Through the integration of information gathered from the four approaches, the application of this model returns a precise and detailed view of the infectious risk and of the microbial ecology of the ICU

Early and late improvement of global and regional left ventricular function after transcatheter aortic valve implantation in patients with severe aortic stenosis: an echocardiographic study.

The recent development of transcatheter aortic valve implantation (TAVI) for severe aortic stenosis (AS) treatment offers a viable option for high-risk patient categories. Our aim is to evaluate whether 2D strain and strain rate can detect subtle improvement in global and regional LV systolic function immediately after TAVI. 2D conventional and 2D strain (speckle analysis) echocardiography was performed before, at discharge and after three months in thirty three patients with severe AS. After TAVI, we assessed by conventional echocardiography an immediate reduction of transaortic peak pressure gradient (p<0.0001), of mean pressure gradient (p<0.0001) and a concomitant increase in aortic valve area (AVA: 1.08±0.31 cm(2)/m(2); p<0.0001). 2D longitudinal systolic strain showed a significant improvement in all patients, both at septal and lateral level, as early as 72 h after procedure (septal: -14.2±5.1 vs -16.7±3.7%, p<0.001; lateral: -9.4±3.9 vs -13.1±4.5%, p<0.001; respectively) and continued at 3 months follow-up (septal: -18.1±4.6%, p<0.0001; lateral: -14.8±4.4%, p<0.0001; respectively). Conventional echocardiography after TAVI proved a significant reduction of LV end-systolic volume and of LV mass with a mild improvement of LV ejection fraction (EF) (51.2±11.8 vs 52.9±6.4%; p<0.02) only after three months. 2D strain seems to be able to detect subtle changes in LV systolic function occurring early and late after TAVI in severe AS, while all conventional echo parameters seem to be less effective for this purpose. Further investigations are needed to prove the real prognostic impact of these echocardiographic findings

Severe Aortic Stenosis and Myocardial Function: Diagnostic and Prognostic Usefulness of Ultrasonic Integrated Backscatter Analysis

Background— The aim of this study was to assess the myocardial reflectivity pattern in severe aortic valve stenosis through the use of integrated backscatter (IBS) analysis. Patients with aortic stenosis (AS) were carefully selected in the Department of Cardiology. Methods and Results— Thirty-five subjects (AS: valve orifice ≤1 cm2; 12 female; mean age, 71.8±6.2 years) and 25 healthy subjects were studied. All subjects of the study had conventional 2D-Doppler echocardiography and IBS. Backscatter signal was sampled at the septum and posterior wall levels. Patients with AS were divided into 2 groups: 16 patients with initial signs of congestive heart failure and a depressed left ventricular systolic function (DSF) (ejection fraction [EF] range, 35% to 50%) and 19 asymptomatic patients with normal left ventricular systolic function (NSF) (EF >50%). Myocardial echo intensity (pericardium related) was significantly higher at the septum and posterior wall levels in DSF than in NSF and in control subjects. IBS variation, as an expression of variation of the signal, appeared to be significantly lower in AS with DSF than in NSF and in control subjects, at both the septum and posterior wall levels. Patients with DSF underwent aortic valve replacement, and, during surgical intervention, a septal myocardial biopsy was made for evaluation of myocardium/fibrosis ratio. Abnormally increased echo intensity was detected in left ventricular pressure overload by severe aortic stenosis and correlated with increase of myocardial collagen content (operating biopsy). Conclusions— One year after aortic valve replacement, we observed a significant reduction of left ventricular mass, and, only if pericardial indexed IBS value (reduction of interstitial fibrosis) decreased, it was possible to observe an improvement of EF and of IBS variation

The 2012 Emilia seismic sequence (Northern Italy): Imaging the thrust fault system by accurate aftershock location

Starting from late May 2012, the Emilia region (Northern Italy) was severely shaken by an intense seismic sequence, originated from a ML 5.9 earthquake on May 20th, at a hypocentral depth of 6.3 km, with thrusttype focal mechanism. In the following days, the seismic rate remained high, counting 50 ML ≥ 2.0 earthquakes a day, on average. Seismicity spreads along a 30 km east–west elongated area, in the Po river alluvial plain, in the nearby of the cities Ferrara and Modena. Nine days after the first shock, another destructive thrust-type earthquake (ML 5.8) hit the area to the west, causing further damage and fatalities. Aftershocks following this second destructive event extended along the same east-westerly trend for further 20 km to the west, thus illuminating an area of about 50 km in length, on thewhole. After the first shock struck, on May 20th, a dense network of temporary seismic stations, in addition to the permanent ones, was deployed in the meizoseismal area, leading to a sensible improvement of the earthquake monitoring capability there. A combined dataset, including threecomponent seismic waveforms recorded by both permanent and temporary stations, has been analyzed in order to obtain an appropriate 1-D velocity model for earthquake location in the study area. Here we describe the main seismological characteristics of this seismic sequence and, relying on refined earthquakes location, we make inferences on the geometry of the thrust system responsible for the two strongest shocks

The 2012 Emilia seismic sequence (Northern Italy): Imaging the thrust fault system by accurate aftershock location

Starting from late May 2012, the Emilia region (Northern Italy) was severely shaken by an intense seismic sequence, originated from a ML 5.9 earthquake on May 20th, at a hypocentral depth of 6.3 km, with thrusttype focal mechanism. In the following days, the seismic rate remained high, counting 50 ML ≥ 2.0 earthquakes a day, on average. Seismicity spreads along a 30 km east–west elongated area, in the Po river alluvial plain, in the nearby of the cities Ferrara and Modena. Nine days after the first shock, another destructive thrust-type earthquake (ML 5.8) hit the area to the west, causing further damage and fatalities. Aftershocks following this second destructive event extended along the same east-westerly trend for further 20 km to the west, thus illuminating an area of about 50 km in length, on thewhole. After the first shock struck, on May 20th, a dense network of temporary seismic stations, in addition to the permanent ones, was deployed in the meizoseismal area, leading to a sensible improvement of the earthquake monitoring capability there. A combined dataset, including threecomponent seismic waveforms recorded by both permanent and temporary stations, has been analyzed in order to obtain an appropriate 1-D velocity model for earthquake location in the study area. Here we describe the main seismological characteristics of this seismic sequence and, relying on refined earthquakes location, we make inferences on the geometry of the thrust system responsible for the two strongest shocks.Published44-552T. Tettonica attivaJCR Journalope

On Quantum Effects in Soft Leptogenesis

It has been recently shown that quantum Boltzman equations may be relevant for leptogenesis. Quantum effects, which lead to a time-dependent CP asymmetry, have been shown to be particularly important for resonant leptogenesis when the asymmetry is generated by the decay of two nearly degenerate states. In this work we investigate the impact of the use of quantum Boltzman equations in the framework ``soft leptogenesis'' in which supersymmetry soft-breaking terms give a small mass splitting between the CP-even and CP-odd right-handed sneutrino states of a single generation and provide the CP-violating phase to generate the lepton asymmetry.Comment: 15 pages, 4 figures. Replacement to match published versio