49 research outputs found
Young camel ceruloplasmin : purification and partial characterization
La céruloplasmine d'un chamelon âgé de six mois a été isolée et purifiée en une seule étape, utilisant une chromatographie sur Sépharose activée par de la chloroéthylamine. La masse moléculaire de la protéine a été déterminée par électrophorèse avec SDS et a été estimée à 130 000 Da. La protéine possède une mobilité électrophorétique légèrement supérieure à celle de l'homme, ce qui suggère que la céruloplasmine du chamelon est compacte et plus acide. Le nombre d'atomes de cuivre par molécule de céruloplasmine a été de 5,8 ± 0,3. Le spectre optique de la céruloplasmine du chamelon a montré une absorption maximale à 610 nm attribuée au cuivre de type 1 . Le spectre EPR a été totalement dépourvu d'un signal correspondant au cuivre de type 2. Les paramètres cinétiques de l'activité oxidasique, utilisant la p-phénylendiamine comme substrat, ont été déterminés : Km = 0,42 µM NADH/mn/mg céruloplasmine et Vmax = 0,93. Le pH optimal de l'activité a été de 5,7
Some factors affecting dairy she-camel performance
Abstract In order to determine the effect of some factors, as different levels of energy and protein, milking interval, lactation stage, and lactation rank on she-camel performance after weaning of camel-calves, 20 lactating shecamels were divided into four groups, 5 animals each, according to age and weight at last parturition. Groups had randomly allocated to one of four treatments diets. Group A received diet with 13% Crude Protein (CP) and 2.4 MCal Metabolisable Energy (ME). Group B received diet with 13% CP and 3.0 MCal ME. Group C and D received diet with 15% CP and 2.4, 3.0 MCal ME respectively. Diets contain 35/65 (roughage/concentrate, respectively). After 14 days of adaptation period, individual feed offered and orts had been recorded, daily and continued up to entire experimental period of 10 months. Milk yield was recorded two milking time from three consecutive days. The results show that diet (B) gave higher milk yield (MY), Fat Corrected Milk (FCM), Energy Corrected Milk (ECM) and Feed Conversion Ratio (FCR) 8.32, 11.77, 7.47, and 1.38 respectively. Diet (A) has higher fat % content when comparing with the other diets. Treatment did not affect milk composition except on fat and ash percentage. Milk secretion rate for 10 hours milking interval "evening milking" was higher comparing with 14 hours milking interval "morning milking" 397, 353 g/h respectively. Maximum MY, FCM and ECM were at mid lactation. In late lactation MY, FCM, ECM decreased. Higher milk productivity was at 3rd and 6th season of lactation