Temperature rather than photoperiod controls growth cessation and dormancy in Sorbus species

Environmental regulation of growth and dormancy of four Sorbus genotypes was studied in controlled environments. Emphasis was placed on assessment of the presence and nature of the deficient photoperiodic dormancy regulation system that has previously been reported for some woody Rosaceae species. Two genotypes of Sorbus aucuparia L. maintained indeterminate growth for 8 weeks and 9 weeks at temperatures of 15 °C and 21 °C in both 20 h and 10 h photoperiods, while at 9 °C, in the same photoperiodic conditions, they immediately ceased growing. At the higher temperatures, initiation of new leaves (nodes) was unaffected by photoperiod, while internode elongation was significantly enhanced by long days (LD). However, even after prolonged exposure to 9 °C, most plants resumed growth when moved to high temperature and LD, indicating a shallow state of dormancy. Seedlings of Sorbus intermedia (J. F. Ehrh.) Pers. and micro-propagated plantlets of S. commixta Hedl. ‘Dodong’ were also unaffected by photoperiod during primary growth, but failed to elongate and gradually became dormant regardless of temperature and day-length conditions. However, after chilling and breaking of dormancy, the plants elongated vigorously but changed to a determinate mode of growth. Furthermore, a temperature of 9 °C was found to be fully effective for breaking dormancy in S. intermedia plants. It is concluded that deficient photoperiodic dormancy control seems widespread in the Rosaceae and that, in such plants, both dormancy induction and release is brought about by low temperature. The potential impacts of climate change on such trees are discussed

Haptic Control of Multistate Generative Music Systems

Force-feedback controllers have been considered as a solution to the lack of sonically coupled physical feedback in digital-music interfaces, with researchers focusing on instrument-like models of interaction. However, there has been little research applied to the use of force-feedback interfaces to the control of real-time generative-music systems. This paper proposes that haptic interfaces could enable performers to have a more fully embodied engagement with such systems, increasing expressive control and enabling new compositional and performance potentials. A proof-of-concept project is described, which entailed development of a core software toolkit and implementation of a series of test cases

Teacher questioning to elicit students’ mathematical thinking in elementary school classrooms

Cognitively guided instruction (Cgi) researchers have found that while teachers readily ask initial questions to elicit students' mathematical thinking, they struggle with how to follow up on student ideas. This study examines the classrooms of three teachers who had engaged in algebraic reasoning Cgi professional development. We detail teachers' questions and how they relate to students' making explicit their complete and correct explanations. We found that after the initial "How did you get that?" question, a great deal of variability existed among teachers' questions and students' responses

SimShiftDB; local conformational restraints derived from chemical shift similarity searches on a large synthetic database

We present SimShiftDB, a new program to extract conformational data from protein chemical shifts using structural alignments. The alignments are obtained in searches of a large database containing 13,000 structures and corresponding back-calculated chemical shifts. SimShiftDB makes use of chemical shift data to provide accurate results even in the case of low sequence similarity, and with even coverage of the conformational search space. We compare SimShiftDB to HHSearch, a state-of-the-art sequence-based search tool, and to TALOS, the current standard tool for the task. We show that for a significant fraction of the predicted similarities, SimShiftDB outperforms the other two methods. Particularly, the high coverage afforded by the larger database often allows predictions to be made for residues not involved in canonical secondary structure, where TALOS predictions are both less frequent and more error prone. Thus SimShiftDB can be seen as a complement to currently available methods

Teacher questioning to elicit students’ mathematical thinking in elementary school classrooms

Downloaded from 380 Cognitively guided instruction (Cgi) researchers have found that while teachers readily ask initial questions to elicit students' mathematical thinking, they struggle with how to follow up on student ideas. This study examines the classrooms of three teachers who had engaged in algebraic reasoning Cgi professional development. We detail teachers' questions and how they relate to students' making explicit their complete and correct explanations. We found that after the initial "How did you get that?" question, a great deal of variability existed among teachers' questions and students' responses

Excretion and folding of plasmalemma function to accommodate alterations in guard cell volume during stomatal closure in Vicia faba L.

Stomatal movement results in large and repetitive changes in cell volume and consequently surface area. While endocytosis has been extensively studied and is thought to be a major mechanism for accommodating the volume changes as evidenced mainly by fluorescent labelling and confocal imaging, studies at the ultrastructural level in intact guard cells of stomata regulated by natural factors have never been reported. Here, it is reported that excretion and folding of the plasmalemma are critical for accommodating the volume alterations in intact guard cells in Vicia faba L. Using transmission electron microscopy in combination with laser confocal microscopy, it was observed that in fully opened stomata the plasmalemma was smooth and tightly adhered to the cell walls while a whole large vacuole appeared in the cell. In the closed stomata, besides vacuole fragmentation, endocytosis of the tonoplast rather than the plasmalemma commonly occurred. Importantly, in stomata where pore closure was induced by circadian rhythm or CO2, numerous tiny vesicles were found outside the plasmalemma and, moreover, extensive folding of the plasmalemma could also be found in some regions of the cells. Additionally, an unknown structure was found at the interface between the plasmalemma and cell walls, especially in those areas of the cell where extensive folding occurred, suggesting that plasmalemma turnover is possibly associated with an interaction between the plasmalemma and cell walls. Collectively, the results strongly indicate that excretion and folding of the plasmalemma are critical for the accommodation of the cell volume alterations during stomatal movement

Arabidopsis annexin1 mediates the radical-activated plasma membrane Ca2+ - and K+ -permeable conductance in root cells

Plant cell growth and stress signaling require Ca2+ influx through plasma membrane transport proteins that are regulated by reactive oxygen species. In root cell growth, adaptation to salinity stress, and stomatal closure, such proteins operate downstream of the plasma membrane NADPH oxidases that produce extracellular superoxide anion, a reactive oxygen species that is readily converted to extracellular hydrogen peroxide and hydroxyl radicals, OH_. In root cells, extracellular OH_ activates a plasma membrane Ca2+-permeable conductance that permits Ca2+ influx. In Arabidopsis thaliana, distribution of this conductance resembles that of annexin1 (ANN1). Annexins are membrane binding proteins that can form Ca2+-permeable conductances in vitro. Here, the Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal OH_-activated Ca2+- and K+-permeable conductance. This manifests in both impaired root cell growth and ability to elevate root cell cytosolic free Ca2+ in response to OH_. An OH_-activated Ca2+ conductance is reconstituted by recombinant ANN1 in planar lipid bilayers. ANN1 therefore presents as a novel Ca2+-permeable transporter providing a molecular link between reactive oxygen species and cytosolic Ca2+ in plants

Dynamics of value-tracking in financial markets

The effciency of a modern economy depends on value-tracking: that market prices of key assets broadly track some underlying value. This can be expected if a suffcient weight of market participants are valuation- based traders, buying and selling an asset when its price is, respectively, below and above their well-informed private valuations. Such tracking will never be perfect, and we propose a natural unit of tracking error, the 'deciblack' . We then use a simple discrete-time model to show how large tracking errors can arise if enough market participants are not valuation-based traders, regardless of how much information the valuation-based traders have. Similarly to Lux [17] and others who study subtly different models, we find a threshold above which value-tracking breaks down without any changes in the underlying value of the asset. We propose an estimator of the tracking error and establish its statistical properties. Because financial markets are increasingly dominated by non-valuation-based traders, assessing how much valuation-based investing is required for reasonable value tracking is of urgent practical interest