Behaviour of two SCAR markers for seedlessness within Central European varieties of grapevine

Two working sequence-characterised amplified region markers, SCC8 and SCF27, linked to the seedless phenotype and particularly to the major locus involved in this trait, sdI, were identified in grapevine. Several seeded varieties also showed the alleles for seedlessness at these marker loci. Based on comparison of allelic distribution of two markers in a set of several seedless and seeded varieties, as well as three segregating progenies, we provide evidence that 'Chaouch rose', an ancient seeded variety of unknown origin, has potential to promote seedlessness and most probably belongs to the group of seeded varieties that harbour the sdI+ allele. We conclude that using both SCC8 and SCF27 and, in special cases, even their null alleles can help to elucidate the seedlessness of individuals that lack the amplicon accountable for seedlessness at one marker locus. However, the presence of null alleles and the genetic distance of markers from the sdI locus involved in seedlessness may cause complications.

Design of deeply cooled ultra-low dissipation amplifier and measuring cell for quantum measurements with a microwave single-photon counter

The requirements and details of designing a measuring cell and low-back-action deeply-cooled amplifier for quantum measurements at 10 mK are discussed. This equipment is a part of a microwave single-photon counter based on a superconducting flux qubit. The high electron mobility transistors (HEMTs) in the amplifier operate in unsaturated microcurrent regime and dissipate only 1 microwatt of dc power per transistor. Simulated amplifier gain is 15 dB at 450 MHz with a high-impedance (~5 kOhm signal source and standard 50-Ohm output.Comment: 10 pages, 7 figures. To be published in Fizika Nizkikh Temperatur (Low Temperature Physics) vol. 50, No.1 (2024

The demonstration of the GFLV Nepovirus isolates on naturally infected grapevine cultivars and evaluation of variability within genome region encoding movement protein

In this work, the infection of Grapevine fanleaf virus (GFLV) disease was described on the base of symptomatic differences within eight grapevine plants of six grape ciltivars with positive tests on GFLV. Among them, cultivars Kodrjanka, Pamjati Negrula, Kišmiš Lučistyj were planted in wine region of the South Moravia (Czech Republic), three infected grapevine cultivars (URS, Cinsaut, Dimrit) included in this study originating from Italy. Except symptomatic evaluation, the differences between isolates were emphasized at the genetic level too, exactly in the frame of RNA2 genomic region coding movement protein. The variability of the tested isolates within the eight plants was in the range from 86.59 to 97.61% at the nucleotide level. The results confirmed very high degree of similarity between virus isolates of GFLV within studied RNA2 region. This fact was assessed by the phylogenetic analysis of obtained sequencing data too

Regional typology of Poland

Секция 1. Региональные проблемы социально-экономического развити

Ocena różnych metod ekstrakcji dna w celu wykrycia bakterii Xanthomonas campestris pv. Campestris w liściach kapusty

The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determination of Xccon the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three sets of primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv. campestris detection protocol were recommended – commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.Bakteria Xanthomonas campestris pathovar campestris (Xcc) jako przyczyna czarnej zgnilizny roślin z rodziny krzyżowych powoduje znaczne straty w plonie rolniczym na całym świecie. Zwalczanie czarnej zgnilizny, a także określenie Xcc na podstawie parametrów morfologicznych lub za pomocą testów patogeniczności jest trudne. W celu zoptymalizowania protokołu PCR przetestowano dziesięć różnych możliwości ekstrakcji DNA, a następnie metodę PCR. Na podstawie walidowanej metody ISTA za-stosowano trzy zestawy primerów, mianowicie UBP 1052F-BACR, DLH 120-125 i ZUP 2309-2310. Porównano wyniki zmierzonego stężenia i jakości DNA oraz skuteczności dla amplifikacji PCR. Zalecono trzy sposoby ekstrakcji DNA w ramach protokołu detekcji Xanthomonas campestris pv. Campestris: zestaw komercyjny używany do izolacji z tkanek według Macherey-Nagel, MO BIO oraz zestaw dla hodowli mikrobowych według MO BIO