Computational binding mechanism of Mycobacterium tuberculosis UDP-NAG enolpyruvyl transferase (MurA) with inhibitors fosfomycin, cyclic disulfide analog RWJ-3981, pyrazolopyrimidine analog RWJ-110192, purine analog RWJ-140998, 5-sulfonoxy-anthranilic aci

Worldwide, tuberculosis (TB) remains the most frequent and important infectious disease causing morbidity and death. One-third of the world's population is infected with Mycobacterium tuberculosis (Mtb), the etiologic agent of TB. In this context, TB is in the top three, with malaria and HIV being the leading causes of death from a single infectious agent, and about two million deaths are attributable to TB annually. The bacterial enzyme MurA catalyzes the transfer of enolpyruvate from phosphoenolpyruvate (PEP) to uridine diphospho-N-acetylglucosamine (UNAG), which is the first committed step of bacterial cell wall biosynthesis. In this work, 3D structural model of Mtb-MurA enzyme has been developed, for the first time, by homology modeling and molecular dynamics simulation techniques. The model provided clear insight in its structure features, i.e. substrate binding pocket, and common docking site. Multiple sequence alignment and 3D structure model provided the putative substrate binding pocket of Mtb-MurA with respect to E.coli MurA. This analysis was helpful in identifying the binding sites and molecular function of the MurA homologue. Molecular docking study was performed on this 3D structural model, using different classes of inhibitors like fosfomycin, cyclic disulfide analog RWJ-3981, pyrazolopyrimidine analog RWJ-110192, purine analog RWJ-140998, 5-sulfonoxy-anthranilic acid derivatives T6361, T6362 and the results showed that the 5-sulfonoxyanthranilic acid derivatives is showed best interaction compared with other inhibitor, taking in to this we also design a new efficient analogs of T6361 and T6362 which are showed even better interaction with Mtb-MurA than the parental5-sulfonoxy-anthranilic acid derivatives. Further the comparative molecular electrostatic potential and cavity depth analysis of Mtb-MurA suggested several important differences in its substrate and inhibitor binding pocket. Such differences could be exploited in the future for designing of a more specific inhibitor for Mtb-MurA enzym

Selection of Machining Parameters of Face Milling operation for Aluminium with HSS cutter using Response Surface Methodology and Genetic Algorithm

Components used in chemical equipments are produced from forging, extrusion and casting processes with classic dimension tolerances due to its producing ability. So machining processes were introduced for close tolerance assembly and improve the product working efficiencies. At present,  lot of machining processes are available for producing chemical equipments such as turning, milling, drilling and grinding etc.,. Milling operation is playing critical role on making the chemical equipment’s components with high accuracy and higher productivity. Face milling operation is one of the milling processes which is used for achieving higher flatness and surface finish of chemical equipment’s parts. Thiswork concentrates the parameters influence on Material Removal Rate (MRR) and Surface Roughness (SR) by using aluminium as work piece material. Actually, aluminium alloy has the  most significant in chemical industries because of its inherent properties such as, corrosive resistance , low weight to strength ratio. The milling parameters such as feed rate, spindle speed  and depth of cut are selected as parameters for improving the quality and productivity. This work put together the link between input and response variables for developing the face milling performances. The Response Surface Methodology (RSM) is employ for making the link between dependent and independent variables. Building the empirical model by conducting regression analysis The performance of developed regression models are verified with experimental results. Verification results show the developed models have best agreement with experimental results. The developed models are used for achieving the best input parameters by using Genetic Algorithm (GA). Finally, the optimal parameters are evaluated by GA

A Study on Sanctuary and Seclusion Issues in Internet-of-Things

Internet-of-Things (IoT) are everywhere in our daily life. They are used in our homes, in hospitals, deployed outside to control and report the changes in environment, prevent fires, and many more beneficial functionality. However, all those benefits can come of huge risks of seclusion loss and sanctuary issues. To secure the IoT devices, many research works have been con-ducted to countermeasure those problems and find a better way to eliminate those risks, or at least minimize their effects on the user�s seclusion and sanctuary requirements. The study consists of four segments. The first segment will explore the most relevant limitations of IoT devices and their solutions. The second one will present the classification of IoT attacks. The next segment will focus on the mechanisms and architectures for authentication and access control. The last segment will analyze the sanctuary issues in different layers

Implementation of Driver Software of Trailer Module Chip

The aim of the project is to develop a driver software for UJA1076A SBC in embedded C using IAR Embedded Workbench and integrate the driver software with application software of Trailer module. Currently MC33903 system basis chip from Freescale is used in Trailer Module. As an initiative to reduce the material cost for the Trailer module product, a lower price SBC NXP UJA1076A has been used. Also due to the fact that the newly proposed SBC has less number of operating modes and registers to configure, it helps in making the driver software much more simpler, thus reducing the risk of hidden issues in the otherwise complex design and code of the current SBC driver software

COST-EFFECTIVENESS ANALYSIS IN THE MANAGEMENT OF STROKE

Objective: Stroke is one of the leading causes of death and disabilities worldwide. Cost-effectiveness analysis helps identify neglected opportunities by highlighting interventions that are relatively inexpensive, yet have the potential to reduce the disease burden substantially. In India, there are wide social and economic disparities. Socioeconomic environment influences occupation, lifestyle, and nutrition of social classes which in turn would influence the prevalence and profile of stroke. By reduction of delays in access to hospital and improving provision of affordable treatments can reduce morbidity and mortality in patients with stroke in India. This study is designed to measure and compare the costs (resources consumed) and consequences (clinical, economic, and humanistic) of pharmaceutical products and services and their impact on individuals, healthcare systems and society.Methods: The purpose of this study is to analyze and conduct a cost-effectiveness analysis for the treatment of stroke in Guntur City Hospitals. The patients were treated either with aspirin or clopidogrel. The health outcomes were measured using Modified Rankin Scale, A prominent risk assessment scale for stroke. The pharmacoeconomic data were computed from the patient data collection forms.Result: The incremental cost-effectiveness ratio of aspirin and clopidogrel were calculated to be Rs. 8046.2/year.Conclusion: The study concludes that aspirin has the increased socioeconomic impact when compared to Clopidogrel and we can see that the earlier therapy has supported discharge, home-based rehabilitation along with reduced hospital stay and hence preferable

A randomized controlled study of nebulized 3% saline versus 0.9% saline with adrenaline in the treatment of acute bronchiolitis

Objective: The objective was to determine whether nebulized hypertonic (3%) saline with adrenaline is more effective than nebulized0.9% saline with adrenaline in the treatment of acute bronchiolitis. Materials and Methods: In this randomized, double-blind, controlledstudy 100 patients were randomly allocated into two groups (50 patients in each group). In Group A (normal saline group), 4 ml ofnormal saline (0.9%) and 1 ml of 1:1,000 adrenaline was given as nebulization with oxygen flow of 6-8 L/min. In Group B (hypertonicsaline group), 4 ml of hypertonic saline (3%) and 1 ml of 1:1,000 adrenaline was given as nebulization with oxygen flow of 6-8 L/min.The nebulization was given at an interval of 4 h, 6 times daily till the patient was ready for discharge. Results: The percentageimprovement in clinical severity scores after inhalation therapy was not significant in Group A on 1st-3rd day after admission (3.4%,2.1%, and 4%, respectively). In Group B, significant improvement was observed on these days (7.4%, 8.7%, and 9.9%, respectively,p<0.001). Furthermore, the improvement in clinical severity scores differed significantly on each of these days between the two groups.Using 3% saline decreased the hospitalization stay by 25%, from 3.4±1.7 days in Group A to 2.5±1.4 days in Group B (p<0.05).Conclusion: In the treatment of acute bronchiolitis, 3% saline nebulization with adrenaline decreases the length of hospitalization andsymptoms as compared to 0.9% saline nebulization

Opioid substitution treatment with sublingual buprenorphine in Manipur and Nagaland in Northeast India: what has been established needs to be continued and expanded

Manipur and Nagaland in northeast India report an antenatal HIV prevalence of > 1% and the current HIV prevalence among injecting drug users is 24% and 4.5% respectively. Through support from DFID's Challenge Fund, Emmanuel Hospital Association (EHA) established thirteen drop-in-centres across the two states to deliver opioid substitution treatment with sublingual buprenorphine for 1200 injecting drug users. Within a short span of time the treatment has been found to be attractive to the clients and currently 1248 injecting opioid users are receiving opioid substitution treatment. The project is acceptable to the drug users, the families, the communities, religious as well as the militant groups. The treatment centres operate all days of the week, have trained staff members, utilize standardized protocols and ensure a strict supervised delivery system to prevent illicit diversion of buprenorphine. The drug users receiving the substitution treatment are referred to HIV voluntary counselling and testing. As this treatment has the potential to change HIV related risk behaviours, what has been established in the two states needs to be continued and expanded with the support from the Government of India

Application of Genetic Algorithm Technique for Machining Parameters Optimization in Drilling of Stainless Steel

Abstract This work is aimed at developing relations between the pertinent variables that affect drilling process of stainless steel using artificial neural network. The experiments were conducted on vertical CNC machining centre. The parameters used were spindle speed and feed rate. The effect of machining parameters on entry burr height, exit burr height and surface roughness was experimentally evaluated for different spindle speeds and feed rates. A model was established between the drilling parameters and experimentally obtained data using ANN. The predicted values and measured values are fairly close, which indicates that the developed model can be effectively used to predict the burr height and surface roughness in drilling of stainless steel. Genetic algorithm (GA) technique was used in this work to identify the optimized drilling parameters. Confirmation test was conducted with the optimized parameters and it was found that confirmation test results were similar to that of GA-predicted output values

A Preliminary Study of Blastocystis sp. Isolated from Chicken in Perak And Selangor, Malaysia

ABSTRACT. Blastocystis is considered to be a zoonoses and it is believed that animals such as chicken constitute large reservoirs for human infection via the faecal-oral route. Therefore, Blastocystis infection was surveyed in free-range chicken and cagereared chicken comprising broiler birds for consumption as well as jungle fowls and silkie chicken kept for recreation. Fresh faecal samples collected were examined by wet smear preparation and were cultured in Jones medium supplemented with 10% horse serum. Out of 107 chickens, it was found that most of the free-range chicken was positive for Blastocystis sp. with a high prevalence rate of 80%–100% in village chicken, jungle fowl and white silkie chicken. However, the cage-reared chicken, consisting of broiler chicken had no infection. The vacuolar form was the most common Blastocystis cell form found in cultures, similar to B. hominis. These cells were usually spherical and vary greatly in size, ranging from 10 μm to 30 μm in diameter. Owing to the free ranging and scavenging habits, the likelihood of acquiring the infection from the environment contaminated with the faecal material of animals with Blastocystis is high in free-range chicken as compared to caged chicken