Detection of Airborne Allegen Phl p5 and its Correlation with Poaceae Pollen Counts in Evora, South Portugal

Introduction: Airborne pollen of grasses (Poaceae family) is the main aeroallergen in many european countries, namely in Portugal. Their pollen can be found in the air all over the year but higher concentrations are reached in springtime (Mars to June). Exposure to its allergens is deduced from pollen counts of environmental air samples. The aim of this study is to analyse the relationship between these pollen counts and the concentration of allergen Phl p5, through a new sampling technology, developed under an European LIFE/Environment program. Methods: Airborne pollen were monitored with a Hirst-type sampler (Burkard 7-day pollen trap). Simultaneously, daily air samples were collected with a high volumetric cyclone-type sampler (Coriolis ® d by Bertin Technologies, France) and Phl p5 concentration was measured from liquid samples through a “sandwich” ELISA with a kit from Indoor Biotecnologies®. Both samplers were placed side by side on a meteorological platform at the town center of Evora, 17 m above ground level and 320 m above sea level. ELISA analysis were performed on samples collected between the 2th of Mars and 12 th of June 2007, which includes the main pollen season for grasses and Phleum pratense. Results: Pollen counts from both samplers were not correlated, with absolute values higher in Burkard sampler. Phl p5 concentration in air samples followed the same features of cyclone-type sampler but it was quantified even when pollen counts were low or null, particularly in the beginning of pollen season. Several peaks of allergen were detected at the end of March and on the 1st - 2nd of April. Conclusions: Coriolis ® d cyclone sampler allowed the quantification of Poaceae either by pollen counts and by an ELISA assay but further analysis on the efficiency of sampling and its relationship with biophysical parameters are needed. These results suggest that pollen counts may not reflect exposure to Poaceae pollen. Immuno-sampling could provide a better understanding of exposure to airborne pollen allergens, namely out of the pollen season

The exotic invasive plant Vincetoxicum rossicum is a strong competitor even outside its current realized climatic temperature range

Dog-strangling vine (Vincetoxicum rossicum) is an exotic plant originating from Central and Eastern Europe that is becoming increasingly invasive in southern Ontario, Canada. Once established, it successfully displaces local native plant species but mechanisms behind this plant’s high competitive ability are not fully understood. It is unknown whether cooler temperatures will limit the range expansion of V. rossicum, which has demonstrated high tolerance for other environmental variables such as light and soil moisture. Furthermore, if V. rossicum can establish outside its current climatic limit it is unknown whether competition with native species can significantly contribute to reduce fitness and slow down invasion. We conducted an experiment to test the potential of V. rossicum to spread into northern areas of Ontario using a set of growth chambers to simulate southern and northern Ontario climatic temperature regimes. We also tested plant-plant competition by growing V. rossicum in pots with a highly abundant native species, Solidago canadensis, and comparing growth responses to plants grown alone. We found that the fitness of V. rossicum was not affected by the cooler climate despite a delay in reproductive phenology. Growing V. rossicum with S. canadensis caused a significant reduction in seedpod biomass of V. rossicum. However, we did not detect a temperature x competition interaction in spite of evidence for adaptation of S. canadensis to cooler temperature conditions. We conclude that the spread of V. rossicum north within the tested range is unlikely to be limited by climatic temperature but competition with an abundant native species may contribute to slow it down

On Logical Depth and the Running Time of Shortest Programs

The logical depth with significance $b$ of a finite binary string $x$ is the shortest running time of a binary program for $x$ that can be compressed by at most $b$ bits. There is another definition of logical depth. We give two theorems about the quantitative relation between these versions: the first theorem concerns a variation of a known fact with a new proof, the second theorem and its proof are new. We select the above version of logical depth and show the following. There is an infinite sequence of strings of increasing length such that for each $j$ there is a $b$ such that the logical depth of the $j$th string as a function of $j$ is incomputable (it rises faster than any computable function) but with $b$ replaced by $b+1$ the resuling function is computable. Hence the maximal gap between the logical depths resulting from incrementing appropriate $b$'s by 1 rises faster than any computable function. All functions mentioned are upper bounded by the Busy Beaver function. Since for every string its logical depth is nonincreasing in $b$, the minimal computation time of the shortest programs for the sequence of strings as a function of $j$ rises faster than any computable function but not so fast as the Busy Beaver function.Comment: 12 pages LaTex (this supercedes arXiv:1301.4451

Abnormal regulation of Na,K-ATPase in Glucose Intolerant Rats.

Introduction: Glucose is the most important physiological insulin secretagogue. However, the mechanisms underlying glucose-induced insulin release are not fully understood. The role of electrogenic systems such as ionic pumps, to these events remains essentially uninvestigated. Na,K-ATPase, responsible for maintaining Na+ and K+ gradients across the plasma membrane and generates a net outward current, thus changes in its activity may contribute to the early ionic events regulating insulin secretion (Therien and Blostein, 2000). Objective: The aim of this work was to evaluate the regulation of Na,K-ATPase activity by glucose in intact -cells of normal and glucose intolerant (GI) rats and its putative contribution to the regulation of insulin secretion. Material and Methods: Pancreatic -cells, from normal or control or GI rats, were isolated and cultured (48h). Cell batches were pre-incubated (30min) with 2mM glucose to reach basal. Afterwards cells were challenged with glucose in the interval 0-11mM for 60min, for dose-dependence evaluation, or with 8mM glucose for 5-120min, for time-dependence evaluation. ATPase activity was assessed in intact cells by colorimetric quantification of Pi formed in 30min. Na,K-ATPase activity was calculated by the difference between the activities obtained in the absence and in presence the of 1mM ouabain (Costa et al., 2009). Results: In β-cells from normal rats, glucose induced a bimodal regulation of Na,K-ATPase. In the absence of glucose, Na,K-ATPase activity was 0.056±0.015 U/mg. Stimulation with 2mM glucose induced an increase of Na,K-ATPase activity of ~4 fold whereas for [glucose] above 2mM it was observed a significant inhibition of Na,K-ATPase activity (0.061±0.013, 0.080±0.009 and 0.064±0.005 U/mg for 5.6, 8.4 and 11mM glucose, respectively, compared to 0.188±0.035 U/mg observed in 2mM G; n=3-8). β-cells from GI rats does not present this profile; in the absence of glucose, Na,K-ATPase activity was 0.202±0.036 U/mg and no significant differences from this value were observed with the other glucose concentration tested. Addicionally, in β-cells from normal rats, glucose (8mM) induced a time-dependent inhibition, with a biphasic profile, of Na,K-ATPase - it was observed a decrease in the pump activity between 0 and 20min stimulation where it reached a minimum value (77%). For incubation periods over 20min, the pump activity slowly and partially recovered (54%, 55% and 52%, for 30, 60 and 120min, respectively; n=7). In β-cells from GI animals, an less accentuated decrease of Na,K-ATPase activity between 0 ans 20min was also observed (34%), and is not observed further recover in activity. Conclusions: This work demonstrates there Na,K-ATPase is strictly regulated by glucose in pancreatic β-cell. This regulation is unpaired in GI animals. Na,K-ATPase contribution to glucose-induced ionic events and insulin secretion might be relevant and must be explored as a possible therapeutic target in TD2 . 1. Therien AG, Blostein R (2000) Mechanisms of sodium pump regulation. Am J Physiol Cell Physiol 279:C541-C566 2. Costa AR, Real J, Antunes CM, Cruz-Morais J (2009) A new approach for determination of Na,K-ATPase activity: application to intact pancreatic beta-cells. In Vitro Cell Dev Biol Ani

Implication of AMPK in glucose-evoked modulation of Na,K-ATPase

Background and aims: Na,K-ATPase is an integral membrane protein that maintains the gradients of Na+ and K+, using the energy of ATP hydrolysis, maintaining the ionic gradients that allow electrical activity to occur. It has been demonstrated that, in pancreatic β-cells, Na,K-ATPase is regulated by glucose and that this phenomenon is impaired in glucose intolerant subjects. However, the mechanism underlying glucose-induced modulation of Na,K-ATPase is still unclear. The AMP-activated protein kinase (AMPK) is a molecular key player in energy homeostasis, providing exquisite sensitivity to small changes in intracellular AMP levels and thus to intracellular [ATP]/[ADP] ratio, that is known to activate protein regulatory pathways. Since in pancreatic β-cell, glucose has marked effects on oxidative metabolism and total intracellular ATP and AMP levels, the involvement of AMPK in the cascade of events regulating Na,K-ATPase regulation in pancreatic β-cells was postulated. The aim of this work was to evaluate the putative role of AMPK in the glucose-evoked regulation of Na,K-ATPase activity in the pancreatic β-cell. Materials and methods: Pancreatic -cells from normal (control) or glucose-intolerant Wistar rats (GIR) were isolated and cultured (48h). Cell batches were pre-incubated (30min) with 2.1mM glucose to reach basal activity. Afterwards cells were challenged to 8.4mM glucose for 20min, in the presence or absence of AMPK agonists (AICAR) and antagonists (compound C; CC). ATPase activity was assessed in intact cells by colorimetric quantification of Pi formed in 30min. Na,K-ATPase activity was calculated by the difference between the activities obtained in the absence and in presence the of 1mM ouabain. Results: In basal conditions the activity of Na,K-ATPase from normal and GIR pancreatic β-cell was similar (0.184±0.030 and 0.186±0.020 molPi/min/mgProt, respectively). Challenging the control β-cells with glucose 8.4mM evoked a 62% reduction of Na,K-ATPase activity whereas in GIR β-cells a significantly lower inhibition (40%) was observed. The addition of AICAR 1mM abolished glucose-induced Na,K-ATPase inhibition (0,166±0.011 molPi/min/mg). In control β-cell, the addition of CC 10 μM had no effect on glucose-induced inhibition of Na,K-ATPase. In the contrary, in GIR β-cells it significantly potentiated glucose-evoked inhibition of Na,K-ATPase reaching values similar to that observed in the controls (66%). Conclusions: The AMPK agonist AICAR counteracts the inhibitory action of glucose on Na,K-ATPase of control β-cells whereas CC amplified the glucose-induced inhibition of Na,K-ATPase in GIR β-cells. These results suggest that AMPK plays a central role in the cascade of events underlying glucose-induced modulation of Na,K-ATPase and that the defect must be upstream of AMPK. Finally, abnormal glucose-induced regulation of Na,K-ATPase occurs prior to overt type 2 diabetes and might be a feature in the disease development

The Thermodynamics of Cosmic String densities in U(1) Scalar Field Theory

We present a full characterization of the phase transition in U(1) scalar field theory and of the associated vortex string thermodynamics in 3D. We show that phase transitions in the string densities exist and measure their critical exponents, both for the long string and the short loops. Evidence for a natural separation between these two string populations is presented. In particular our results strongly indicate that an infinite string population will only exist above the critical temperature. Canonical initial conditions for cosmic string evolution are show to correspond to the infinite temperature limit of the theory.Comment: 4 pages, 4 figures, RevTe