Differential interferometric phases at high spectral resolution as a sensitive physical diagnostic of circumstellar disks

Context. The circumstellar disks ejected by many rapidly rotating B stars (so-called Be stars) offer the rare opportunity of studying the structure and dynamics of gaseous disks at high spectral as well as angular resolution. Aims. This paper explores a newly identified effect in spectro-interferometric phase that can be used for probing the inner regions of gaseous edge-on disks on a scale of a few stellar radii. Methods. The origin of this effect (dubbed central quasi-emission phase signature, CQE-PS) lies in the velocity-dependent line absorption of photospheric radiation by the circumstellar disk. At high spectral and marginal interferometric resolution, photocenter displacements between star and isovelocity regions in the Keplerian disk reveal themselves through small interferometric phase shifts. To investigate the diagnostic potential of this effect, a series of models are presented, based on detailed radiative transfer calculations in a viscous decretion disk. Results. Amplitude and detailed shape of the CQE-PS depend sensitively on disk density and size and on the radial distribution of the material with characteristic shapes in differential phase diagrams. In addition, useful lower limits to the angular size of the central stars can be derived even when the system is almost unresolved. Conclusions. The full power of this diagnostic tool can be expected if it can be applied to observations over a full life-cycle of a disk from first ejection through final dispersal, over a full cycle of disk oscillations, or over a full orbital period in a binary system

Be Stars: Rapidly Rotating Pulsators

I will show that Be stars are, without exception, a class of rapidly rotating stars, which are in the majority of cases pulsating stars as well, while none of them does possess a large scale (i.e. with significant dipolar contribution) magnetic field.Comment: Review talk given at "XX Stellar Pulsation Conference Series: Impact of new instrumentation and new insights in stellar pulsations", Granada, 5-9 September 2011, in press in AIP Conf. Se

Multitechnique testing of the viscous decretion disk model I. The stable and tenuous disk of the late-type Be star β\beta CMi

The viscous decretion disk (VDD) model is able to explain most of the currently observable properties of the circumstellar disks of Be stars. However, more stringent tests, focusing on reproducing multitechnique observations of individual targets via physical modeling, are needed to study the predictions of the VDD model under specific circumstances. In the case of nearby, bright Be star $\beta$ CMi, these circumstances are a very stable low-density disk and a late-type (B8Ve) central star. The aim is to test the VDD model thoroughly, exploiting the full diagnostic potential of individual types of observations, in particular, to constrain the poorly known structure of the outer disk if possible, and to test truncation effects caused by a possible binary companion using radio observations. We use the Monte Carlo radiative transfer code HDUST to produce model observables, which we compare with a very large set of multitechnique and multiwavelength observations that include ultraviolet and optical spectra, photometry covering the interval between optical and radio wavelengths, optical polarimetry, and optical and near-IR (spectro)interferometry. Due to the absence of large scale variability, data from different epochs can be combined into a single dataset. A parametric VDD model with radial density exponent of $n$ = 3.5, which is the canonical value for isothermal flaring disks, is found to explain observables typically formed in the inner disk, while observables originating in the more extended parts favor a shallower, $n$ = 3.0, density falloff. Modeling of radio observations allowed for the first determination of the physical extent of a Be disk (35$^{+10}_{-5}$ stellar radii), which might be caused by a binary companion. Finally, polarization data allowed for an indirect measurement of the rotation rate of the star, which was found to be $W \gtrsim 0.98$, i.e., very close to critical.Comment: 19 pages (35 including online material), 17 figures, 2 online figures, 2 online tables with dat

Revealing the structure of the outer disks of Be stars

Context. The structure of the inner parts of Be star disks (20 stellar radii) is well explained by the viscous decretion disk (VDD) model, which is able to reproduce the observable properties of most of the objects studied so far. The outer parts, on the ther hand, are not observationally well-explored, as they are observable only at radio wavelengths. A steepening of the spectral slope somewhere between infrared and radio wavelengths was reported for several Be stars that were previously detected in the radio, but a convincing physical explanation for this trend has not yet been provided. Aims. We test the VDD model predictions for the extended parts of a sample of six Be disks that have been observed in the radio to address the question of whether the observed turndown in the spectral energy distribution (SED) can be explained in the framework of the VDD model, including recent theoretical development for truncated Be disks in binary systems. Methods. We combine new multi-wavelength radio observations from the Karl. G. Jansky Very Large Array (JVLA) and Atacama Pathfinder Experiment (APEX) with previously published radio data and archival SED measurements at ultraviolet, visual, and infrared wavelengths. The density structure of the disks, including their outer parts, is constrained by radiative transfer modeling of the observed spectrum using VDD model predictions. In the VDD model we include the presumed effects of possible tidal influence from faint binary companions. Results. For 5 out of 6 studied stars, the observed SED shows strong signs of SED turndown between far-IR and radio wavelengths. A VDD model that extends to large distances closely reproduces the observed SEDs up to far IR wavelengths, but fails to reproduce the radio SED. ... (abstract continues but did not fit here)Comment: 20 pages, 8 figure

Structural and functional characterization of the interaction between the influenza A virus RNA polymerase and the CTD of host RNA polymerase II

Influenza A viruses, causing seasonal epidemics and occasional pandemics, rely on interactions with host proteins for their RNA genome transcription and replication. The viral RNA polymerase utilizes host RNA polymerase II (Pol II) and interacts with the serine 5 phosphorylated (pS5) C-terminal domain (CTD) of Pol II to initiate transcription. Our study, using single-particle electron cryomicroscopy (cryo-EM), reveals the structure of the 1918 pandemic influenza A virus polymerase bound to a synthetic pS5 CTD peptide composed of four heptad repeats mimicking the 52 heptad repeat mammalian Pol II CTD. The structure shows that the CTD peptide binds at the C-terminal domain of the PA viral polymerase subunit (PA-C) and reveals a previously unobserved position of the 627 domain of the PB2 subunit near the CTD. We identify crucial residues of the CTD peptide that mediate interactions with positively charged cavities on PA-C, explaining the preference of the viral polymerase for pS5 CTD. Functional analysis of mutants targeting the CTD-binding site within PA-C reveals reduced transcriptional function or defects in replication, highlighting the multifunctional role of PA-C in viral RNA synthesis. Our study provides insights into the structural and functional aspects of the influenza virus polymerase-host Pol II interaction and identifies a target for antiviral development

Properties and nature of Be stars: 29. Orbital and long-term spectral variations of \gamma\ Cassiopei\ae

A detailed analysis of more than 800 electronic high-resolution spectra of gamma Cas, which were obtained during a time interval of over 6000 days (16.84 yrs) at several observatories, documents the smooth variations in the density and/or extend of its circumstellar envelope. We found a clear anticorrelation between the peak intensity and FWHM of the H alpha emission, which seems to agree with recent models of such emission lines. The main result of this study is a confirmation of the binary nature of the object, determination of a reliable linear ephemeris T_{min.RV} = HJD (2452081.9$\pm$0.6) + (203.52$\pm$0.08)*E, and a rather definitive set of orbital elements. We clearly demonstrated that the orbit is circular within the limits of accuracy of our measurements and has a semi-amplitude of radial-velocity curve of 4.30$\pm$0.09 (km/s). No trace of the low-mass secondary was found. The time distribution of our spectra does not allow a reliable investigation of rapid spectral variations, which are undoubtedly present in the spectra. We postpone this investigation for a future study, based on series of dedicated whole-night spectral observations

Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities

Pervasive transcription of genomes generates multiple classes of non-coding RNAs. One of these classes are stable long non-coding RNAs which overlap coding genes in antisense direction (asRNAs). The function of such asRNAs is not fully understood but several cases of antisense-dependent gene expression regulation affecting the overlapping genes have been demonstrated. Using high-throughput yeast genetics and a limited set of four growth conditions we previously reported a regulatory function for ∼25% of asRNAs, most of which repress the expression of the sense gene. To further explore the roles of asRNAs we tested more conditions and identified 15 conditionally antisense-regulated genes, 6 of which exhibited antisense-dependent enhancement of gene expression. We focused on the sporulation-specific gene SPS100, which becomes upregulated upon entry into starvation or sporulation as a function of the antisense transcript SUT169. We demonstrate that the antisense effect is mediated by its 3' intergenic region (3'-IGR) and that this regulation can be transferred to other genes. Genetic analysis revealed that SUT169 functions by changing the relative expression of SPS100 mRNA isoforms from a short and unstable transcript to a long and stable species. These results suggest a novel mechanism of antisense-dependent gene regulation via mRNA isoform switching