Molecular and Epidemiology Data on Cystic Echinococcosis in Tunisia

Cystic echinococcosis (CE) or hydatidosis is a widespread zoonose in the world. In Tunisia, despite the deployed prevention program, CE remains a serious public health problem. With a human surgical annual incidence averaging 12.6/100000 inhabitants, Tunisia is one of the most endemic areas amongst the Mediterranean countries. Three Echinococcus species have been described: E. granulosus sensu stricto (G1 and G3 genotypes), E. canadensis, and E. equinus. CE, which commonly starts during childhood and described as a young adult disease, may be observed at any age. The liver and the lungs are the most commonly involved organs but the cyst can occur almost anywhere in the body. In production animals, the prevalence of CE is ranged from 16.42% to 40.42% in sheep, 8.56% in cattle, 6% in dromedaries, 2.9% in goats, and 8.48% in donkeys. The elevated number of stray and semi-stray dogs and their frequent contamination by E. granulosus infected viscera is the major cause of the CE spread. A high prevalence of E. granulosus infection has been reported in Tunisian dogs ranging from 3.75% to 27.1%, depending on the regions. Thus, the sanitary education concerning hydatidosis should be reinforced and efforts should be made to implement a targeted educational program

Les chances du libéralisme dans le monde arabo-musulman

On sait que dans le libéralisme, outre la limitation du pouvoir politique, le dernier mot doit revenir à l’individu, à sa liberté, à son choix. Le mot même de liberté s’applique à une relation sociale, c’est-à-dire à une relation entre un ou plusieurs individus. Un individu est libre par rapport à un autre individu, et par extension, par rapport au groupe social, de faire ceci ou cela. Dans le cadre social, la liberté est à la fois, comme dirait F.E.Oppenheim, liberté de et liberté à (freedom..

Reduced-Order Thermal Behavioral Model Based on Diffusive Representation

14 páginas, 15 figuras, 2 tablas.-- et al.The virtual prototyping of power electronic converters requires electrothermal models with various abstraction levels and easy identification. Numerous methods for the construction of compact thermal models have been presented in this paper. Few of them propose state-space models, where the model order can be controlled according to the necessity of the virtual prototyping analyses. Moreover, the model reduction methods require the experience of the engineer and previous calibration. Diffusive representation (DR) is proposed here as an original and efficient method to build compact thermal models as state-space models. The model reduction is obtained through the model parameter identification and/or the time horizon of the measurement data provided for the identification. Instead of eigenvalue elimination, the method enables to specify adequately inside the model the frequency domain wished for the virtual analysis at hand. The proposed method is particularly dedicated to the system optimization phases. Experimental and simulation results are in good agreement. The advantages and limitations of the DR are discussed in comparison to published methods.Peer reviewe

Gas phase ethylene polymerization: What is the influence of condensed mode

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Reduced-Order Thermal Behavioral Model Based on Diffusive Representation

14 páginas, 15 figuras, 2 tablas.-- et al.The virtual prototyping of power electronic converters requires electrothermal models with various abstraction levels and easy identification. Numerous methods for the construction of compact thermal models have been presented in this paper. Few of them propose state-space models, where the model order can be controlled according to the necessity of the virtual prototyping analyses. Moreover, the model reduction methods require the experience of the engineer and previous calibration. Diffusive representation (DR) is proposed here as an original and efficient method to build compact thermal models as state-space models. The model reduction is obtained through the model parameter identification and/or the time horizon of the measurement data provided for the identification. Instead of eigenvalue elimination, the method enables to specify adequately inside the model the frequency domain wished for the virtual analysis at hand. The proposed method is particularly dedicated to the system optimization phases. Experimental and simulation results are in good agreement. The advantages and limitations of the DR are discussed in comparison to published methods.Peer reviewe

Gastrointestinal parasites of canids, a latent risk to human health in Tunisia

Abstract Background Although data on the parasite environmental contamination are crucial to implement strategies for control and treatment, information about zoonotic helminths is very limited in Tunisia. Contamination of areas with canid faeces harboring infective parasite elements represents a relevant health-risk impact for humans. The aim of this study was to assess the environmental contamination with eggs and oocysts of gastrointestinal parasites of dogs and wild canids in Tunisia with special attention to those that can be transmitted to humans. Results One thousand two hundred and seventy faecal samples from stray dogs and 104 from wild canids (red foxes and golden jackals) were collected from different geographical regions throughout Tunisia. The helminth eggs and protozoan oocysts were concentrated by sucrose flotation and identified by microscopic examination. The most frequently observed parasites in dog samples were Toxocara spp. (27.2%), E. granulosus (25.8%), and Coccidia (13.1%). For wild canid faeces, the most commonly encountered parasites were Toxocara spp. (16.3%) followed by Capillaria spp. (9.6%). The parasite contamination of dog faeces varied significantly from one region to another in function of the climate. Conclusion To our knowledge, the study highlights for the first time in Tunisia a serious environmental contamination by numerous parasitic stages infective to humans. Efforts should be made to increase the awareness of the contamination risk of such parasites in the environment and implement a targeted educational program

In vitro scolicidal activity of Thymus capitatus Hoff. et Link. essential oil on Echinococcus granulosus protoscoleces

Cystic echinococcosis remains a serious public health problem worldwide. Surgery is considered as the first option of treatment. Various investigations were carried out to find a convenient scolicidal agent for reducing the risk of post-surgical recurrence of the disease but most of them are associated with adverse side effects. The present study aimed to evaluate the in vitro scolicidal activity of Thymus capitatus Hoff. et Link. essential oil against Echinococcus granulosus protosco- leces. Three concentrations of essential oil (1, 2 and 3 mg/mL) were tested for 1 to 10 min on E. granulosus protosoleces and their viability was assessed by eosin staining test. The essential oil concentrated at 2 and 3 mg/mL killed 100% protoscoleces after 5 and 1 min, respectively. Furthermore, GC/MS and GC-FID analyzes indicated that carvacrol (82.4%) was the major compo- nent of the essential oil. The current investigation showed the potent scolicidal effect of T. capitatus essential oil in vitr

Microsatellite Investigations of Multiple Echinococcus granulosus Sensu Stricto Cysts in Single Hosts Reveal Different Patterns of Infection Events between Livestock and Humans

International audienceCystic echinococcosis (CE) caused by the cestode Echinococcus granulosus sensu lato (s.l.) is a worldwide zoonosis and E. granulosus sensu stricto (s.s.) is the most common species associated with animal and human diseases. The objective of this study was to obtain a better understanding of CE infection in livestock and humans from very low and high endemic areas—France and Tunisia—by studying the genetic diversity of E. granulosus s.s. at the intra-individual host level. This genetic diversity was studied using EgSca6 and EgSca11 microsatellite profiles in 93 sheep from France and Tunisia, and in 12 cattle and 31 children from Tunisia only, all presenting multiple CE cysts (2 to 10 cysts). Overall, 96% of sheep, 92% of cattle, and 48% of children had at least two cysts with different microsatellite profiles. Inversely, 35% of sheep, 17% of cattle, and 65% of children had at least two cysts with the same microsatellite profile. The genotyping results for the CE samples highlight high and similar genetic diversity in France and Tunisia, suggesting that the probability of being successively infected by CE of the same microsatellite profile was rare in both countries. Therefore, our results suggest that in rare cases, several eggs of the same microsatellite profile, from two to seven in our data, can be ingested simultaneously in a single infection event and develop into several cysts in livestock and children. They also indicate that multiple infection events are frequent in livestock, even in a low endemic country such as France, and are less frequent but not negligible in children in a high endemic country such as Tunisia. Moreover, this is the first time that genetic evidence of secondary CE has been found. Further studies are needed to better assess the pattern of infection events in livestock and humans, especially by studying the genetic diversity of adult worms in definitive hosts

Antimicrobial Susceptibility among Pathogens Isolated in Early- versus Late-Onset Ventilator-Associated Pneumonia

Ventilator-associated pneumonia (VAP) is associated with increased hospital stay and high morbidity and mortality in critically ill patients. The aims of this study were to (i) determine the incidence of multidrug-resistant (MDR) pathogens in the first episodes of VAP and to assess potential differences in bacterial profiles of subjects with early- versus late-onset VAP. This was a retrospective cohort study over a period of 18 months including all patients who had a first episode of VAP confirmed by positive bacterial culture. Subjects were distributed into two groups according to the number of intubation days: early-onset VAP (<5 days) or late-onset VAP (≥5 days). The primary endpoint was the nature of causative pathogens and their resistance profiles. Sixty patients were included, 29 men and 31 women, with an average age of 38 ± 16 years. The IGS 2 at admission was 40.5 [32–44] and APACHE was 19 [15–22]. Monomicrobial infections were diagnosed in 77% of patients (n = 46). The most frequently isolated bacteria were A. baumannii, 53% (n = 32); P. aeruginosa in 37% (n = 22); Enterobacterales in 28% (n = 17) and S. aureus in 5% (n = 3). Ninety-seven percent of the bacteria were MDR. The VAP group comprised 36 (60%) episodes of early-onset VAP and 24 (40%) episodes of late-onset VAP. There was no significant difference in the distribution of the bacterial isolates, nor in terms of antibacterial resistances between early- and late-onset VAPs. Our data support recent observations that there is no microbiological difference in the prevalence of potential MDR pathogens or in their resistance profiles associated with early- versus late-onset VAPs, especially in countries with high rates of MDR bacteria