Sirius red staining of the renal cortex.

<p>Representative images of Sirius red staining of the renal cortex; CA: cortical area; JA: juxtamedullary area. A = control males (CA); B = ERA-treated males (CA); C = control females (CA); D = ERA-treated females (CA); E = control males (JA); F = ERA-treated males (JA); G = control females (JA); H = ERA-treated females (JA). ERA: Endothelin receptor antagonist. Total magnification: 400x.</p

Morphometric parameters in juxtamedullary and cortical renal tissue.

<p>Morphometric parameters in juxtamedullary and cortical renal tissue.</p

Number of total glomeruli.

<p>Number of total glomeruli obtained by the maceration method. ERA: Endothelin receptor antagonist. Values are mean ± SEM (n = 10). Data were analyzed using two way ANOVA followed by Bonferroni posttest. Two-way ANOVA showed a statistically significant interaction (p < 0.05) between the effects of ERA treatment and sex. *p<0.05 vs control males.</p

Histological structure of the renal cortex.

<p>Representative images of Hematoxylin-eosin staining of the renal cortex. A = control males; B = ERA-treated males; C = control females; D = ERA-treated females. ERA: Endothelin receptor antagonist. Total magnification: 400x.</p

A: Proteinuria (mg/24 h/100g). B: Creatinine Clearance (ml/min/100g).

<p>ERA: Endothelin receptor antagonist. Values are mean ± SEM. Data were analyzed using two way ANOVA followed by Bonferroni posttest.</p

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NOS activity measured as pmol [¹⁴C] L-citrulline/g tissue/min.

<p>Two-way ANOVA showed no statistically significant interaction between the effects of Diabetes and L-Arg treatment on NOS activity. The effects of Diabetes and L-Arg were considered extremely significant (p<0.001). ***p<0.001 vs. control untreated rats; ^###p<0.001 vs. control rats treated with L-Arg; ^&&&p<0.001 vs. diabetic untreated rats. Data are mean ± SEM (n = 8).</p