An evaluation of corallophelia pertusamucus as an analytical matrix for environmental monitoring: A preliminary proteomic study

For the environmental monitoring of coral, mucus appears to be an appropriate biological matrix due to its array of functions in coral biology and the non-intrusive manner in which it can be collected. The aim of the present study was to evaluate the feasibility of using mucus of the stony coral Lophelia pertusa (L. pertusa) as an analytical matrix for discovery of biomarkers used for environmental monitoring. More specifically, to assess whether a mass-spectrometry-based proteomic approach can be applied to characterize the protein composition of coral mucus and changes related to petroleum discharges at the seafloor. Surface-enhanced laser desorption/ionization–time of flight mass spectrometry (SELDI-TOF MS) screening analyses of orange and white L. pertusa showed that the mucosal protein composition varies significantly with color phenotype, a pattern not reported prior to this study. Hence, to reduce variability from phenotype difference, L. pertusa white individuals only were selected to characterize in more detail the basal protein composition in mucus using liquid chromatography, mass spectrometry, mass spectrometry (LC-MS/MS). In total, 297 proteins were identified in L. pertusa mucus of unexposed coral individuals. Individuals exposed to drill cuttings in the range 2 to 12 mg/L showed modifications in coral mucus protein composition compared to unexposed corals. Although the results were somewhat inconsistent between individuals and require further validation in both the lab and the field, this study demonstrated preliminary encouraging results for discovery of protein markers in coral mucus that might provide more comprehensive insight into potential consequences attributed to anthropogenic stressors and may be used in future monitoring of coral health

A high resolution electromagnetic calorimeter based on lead-tungstate crystals

A large-scale prototype of the PHOS electromagnetic spectrometer, which is part of the ALICE detector, has been built and tested. This prototype has 256 detector channels and is operated at −25 °C. Each detector channel is a lead-tungstate crystal coupled to an Avalanche Photo-Diode with a low-noise preamplifier. The prototype includes a 16×16 crystal matrix, photo-detectors, analog and digital electronics, a thermo-stabilized cooling system, a light-emitting diode monitoring system, and a charged-particle detector acting as veto counter. Results of measurements using electron and hadron beams of the CERN PS and SPS accelerators are discussed, and the performance of the prototype is evaluated