15 research outputs found

    Ruthenibacterium lactatiformans gen. nov., sp.nov., an anaerobic, lactate-producing member of the family Ruminococcaceae isolated from human faeces

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    Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585-1T and 668, were characterized by mesophilic fermentative metabolism, production of d-lactic acid, succinic acid and acetic acid as end products of d-glucose fermentation, prevalence of C18 : 1 ω9, C18 : 1 ω9 aldehyde, C16 : 0 and C16 : 1 ω7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4–56.6 mol%. The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 % similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 % with Gemmiger formicilis ATCC 27749T and 88.9/88.8 % with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae , for which the name Ruthenibacterium lactatiformans gen. nov., sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585-1T (=DSM 100348T=VKM B-2901T)

    Whole genome alignment of complete genome sequences using Mauve.

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    <p>Colored boxes, linear collinear blocks (LCB). White gaps, insertions and deletions. Position atop or below the horizontal line represents the direction of LCB.</p

    Strains, isolated from the same children in different time points.

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    <p>Horizontal axis represent the age of the subject, rows represent the children, labels in the cells correspond to the isolated strains. Dotted lines connect similar strains.</p

    Sizes of pan-genome and core genome of <i>B</i>. <i>longum</i> subsp. <i>longum</i> as functions of the number of strains sequentially added.

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    <p>Each dot represents distribution of data obtained from 10000 random permutations of strain order. Central horizontal lines, medians; lower and upper border of boxes, 25 and 75 quartiles, respectively; ends of the whiskers, minimal and maximal values; black dots, mean values.</p

    Intraspecies Genomic Diversity and Long-Term Persistence of <i>Bifidobacterium longum</i>

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    <div><p>Members of genus <i>Bifidobacterium</i> are Gram-positive bacteria, representing a large part of the human infant microbiota and moderately common in adults. However, our knowledge about their diversity, intraspecific phylogeny and long-term persistence in humans is still limited. <i>Bifidobacterium longum</i> is generally considered to be the most common and prevalent species in the intestinal microbiota. In this work we studied whole genome sequences of 28 strains of <i>B</i>. <i>longum</i>, including 8 sequences described in this paper. Part of these strains were isolated from healthy children during a long observation period (up to 10 years between isolation from the same patient). The three known subspecies (<i>longum</i>, <i>infantis</i> and <i>suis</i>) could be clearly divided using sequence-based phylogenetic methods, gene content and the average nucleotide identity. The profiles of glycoside hydrolase genes reflected the different ecological specializations of these three subspecies. The high impact of horizontal gene transfer on genomic diversity was observed, which is possibly due to a large number of prophages and rapidly spreading plasmids. The pan-genome characteristics of the subspecies <i>longum</i> corresponded to the open pan-genome model. While the major part of the strain-specific genetic loci represented transposons and phage-derived regions, a large number of cell envelope synthesis genes were also observed within this category, representing high variability of cell surface molecules. We observed the cases of isolation of high genetically similar strains of <i>B</i>. <i>longum</i> from the same patients after long periods of time, however, we didn’t succeed in the isolation of genetically identical bacteria: a fact, reflecting the high plasticity of microbiota in children.</p></div

    Pairwise comparison of ortholog groups content in <i>B</i>. <i>longum</i> strains.

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    <p>Heatmap represents the number of shared orthologs between strains, the tree was inferred by complete linkage hierarchical clustering.</p
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