The effect of cultural and environmental factors on potato seed tuber morphology and subsequent sprout and stem development

Seed crops of the variety Estima were grown in each of 2 years using two planting dates, two harvest dates, two plant densities and two irrigation regimes to produce seed tubers which had experienced different cultural and environmental conditions. The effects of these treatments on tuber characteristics, sprout production and stem development in the ware crop were then determined in subsequent experiments using storage regimes of 3 and 10 °C. Time of planting the seed crop affected numbers of eyes, sprouts and above ground stems in the subsequent ware crop because environmental conditions around the time of tuber initiation appeared to alter tuber shape. Cooler, wetter conditions in the 7 days after tuber initiation were associated with tubers which were longer, heavier and had more eyes, sprouts and above ground stems. In contrast, the time of harvesting the seed crop did not affect tuber shape or numbers of above ground stems and there was no interaction with tuber size. The density of the seed crop had no effect on any character measured and irrigation well after tuber initiation did not affect tuber shape, numbers of sprouts or numbers of stems. Seed production treatments, which resulted in earlier dormancy break, were associated with tubers that produced more sprouts and above ground stems, in contrast to the conventional understanding of apical dominance. Storage at 3 °C gave fewer sprouts, a lower proportion of eyes with sprouts and fewer stems than storage at 10 °C. The major effects on stem production appear to result from environmental conditions at the time of tuber initiation of the seed crop and sprouting temperature

Factors affecting color correction of retroreflective markings

A nighttime field study was conducted to assess the effects of retroreflective material area, distribution, and color on judgments of conspicuity. Participants, seated in a stationary vehicle, took part in a pairwise comparison of the stimuli. The independent variables included retroreflective power, area and distribution of the retroreflective material, color of the retroreflective material, participant age, and participant gender. The results indicate that color (white, fluorescent yellow-green, and fluorescent red-orange) was a significant factor in the judgment of conspicuity, as might be predicted from the Helmholtz-Kohlrausch effect. In addition, color interacted with the distribution of material at the high level of retroreflective power. The area of the retroreflective material was also significant. The present study, in agreement with a number of previous studies, indicates that color influences the conspicuity of retroreflective stimuli, but that the results are not always in agreement with color correction factors prescribed in ASTM E 1501. The discrepancy between empirically derived color correction factors seems to be attributable to an interaction of the stimulus size (subtended angle) and color, which previous studies have not extensively examined. To a lesser degree, the retroreflective power of a material also appears to influence conspicuity. While the ASTM correction factors may be appropriate for intermediate subtended solid angles, particularly for nonsaturated colors, smaller correction factors appear appropriate for markings subtending small angles (approaching point sources), and larger factors for larger subtended angles of saturated stimuli.The University of Michigan Industry Affiliation Program for Human Factors in Transportation Safetyhttp://deepblue.lib.umich.edu/bitstream/2027.42/91263/1/102869.pd

Dust devils on Earth and Mars: Extension of particle threshold laboratory simulations

Abstract not available

Regulation of seed germination in the close Arabidopsis relative Lepidium sativum : a global tissue-specific transcript analysis

The completion of germination in Lepidium sativum and other endospermic seeds (e.g. Arabidopsis [Arabidopsis thaliana]) is regulated by two opposing forces, the growth potential of the radicle (RAD) and the resistance to this growth from the micropylar endosperm cap (CAP) surrounding it. We show by puncture force measurement that the CAP progressively weakens during germination, and we have conducted a time-course transcript analysis of RAD and CAP tissues throughout this process. We have also used specific inhibitors to investigate the importance of transcription, translation, and posttranslation levels of regulation of endosperm weakening in isolated CAPs. Although the impact of inhibiting translation is greater, both transcription and translation are required for the completion of endosperm weakening in the whole seed population. The majority of genes expressed during this process occur in both tissues, but where they are uniquely expressed, or significantly differentially expressed between tissues, this relates to the functions of the RAD as growing tissue and the CAP as a regulator of germination through weakening. More detailed analysis showed that putative orthologs of cell wall-remodeling genes are expressed in a complex manner during CAP weakening, suggesting distinct roles in the RAD and CAP. Expression patterns are also consistent with the CAP being a receptor for environmental signals influencing germination. Inhibitors of the aspartic, serine, and cysteine proteases reduced the number of isolated CAPs in which weakening developed, and inhibition of the 26S proteasome resulted in its complete cessation. This indicates that targeted protein degradation is a major control point for endosperm weakening

FLOWERING LOCUS C -dependent and -independent regulation of the circadian clock by the autonomous and vernalization pathways

Background The circadian system drives pervasive biological rhythms in plants. Circadian clocks integrate endogenous timing information with environmental signals, in order to match rhythmic outputs to the local day/night cycle. Multiple signaling pathways affect the circadian system, in ways that are likely to be adaptively significant. Our previous studies of natural genetic variation in Arabidopsis thaliana accessions implicated FLOWERING LOCUS C (FLC) as a circadian-clock regulator. The MADS-box transcription factor FLC is best known as a regulator of flowering time. Its activity is regulated by many regulatory genes in the "autonomous" and vernalization-dependent flowering pathways. We tested whether these same pathways affect the circadian system. Results Genes in the autonomous flowering pathway, including FLC, were found to regulate circadian period in Arabidopsis. The mechanisms involved are similar, but not identical, to the control of flowering time. By mutant analyses, we demonstrate a graded effect of FLC expression upon circadian period. Related MADS-box genes had less effect on clock function. We also reveal an unexpected vernalization-dependent alteration of periodicity. Conclusion This study has aided in the understanding of FLC's role in the clock, as it reveals that the network affecting circadian timing is partially overlapping with the floral-regulatory network. We also show a link between vernalization and circadian period. This finding may be of ecological relevance for developmental programing in other plant species

The next generation of ocular pathogen detection

Metagenomic next-generation sequencing is a powerful method for pathogen detection that combines advanced genome sequencing technology with cutting-edge bioinformatics to analyze microbial populations. Metagenomic next-generation sequencing has the potential to identify uncommon, unculturable, and even previously unidentified pathogens from a clinical isolate. Of particular interest to ophthalmology, this robust data extraction can occur from very small volume clinical samples. Here we discuss the opportunities and limitations of this technique and their current and future application to ophthalmic diagnostics

Driver Distraction: A Naturalistic Observation of Secondary Behaviors With the Use of Driver Assistance Systems

This report describes the naturalistic observation of secondary behaviors performed by 66 drivers who took part in the Automotive Collision Avoidance System Field Operational Test (ACAS FOT). The ACAS FOT included two driver assistance systems, adaptive cruise control (ACC) and forward collision warning (FCW). Each driver participated in both baseline (no driver assistance systems for one week) and treatment conditions (both ACC and FCW available for 3 weeks). The method employed was to sub-sample video data, and code drivers’ secondary behaviors using 4 s video clips of the driver, collected every 5 minutes. Eight-hundred and ninety video clips were reviewed and coded while participants drove manually, with conventional cruise control, ACC, and FCW. The results show that drivers who took part in the field test were no more likely to engage in secondary behaviors when driving with ACC and FCW in comparison to manual control. When the driver assistance systems became available to the participants, there was an increase in the number of conversations drivers had with passengers, probably related to the driver explaining the novel ACAS system to passengers. The results have important implications in that, at least for the duration of exposure examined, they counter the concern often raised that driver assistance systems will promote driver distraction, lapses in attention or modification in perceived risk

Leadership Behaviors and Subordinate Resilience

Utilizing a sample of 150 part-time MBA students, this study evaluated the relationship between leader behaviors and subordinate resilience. We proposed that the transformational leadership dimensions of Attributed Charisma, Idealized Influence, Inspirational Motivation, Intellectual Stimulation, and Individualized Consideration, as well as the transactional leadership dimension of Contingent Reward would be positively associated with subordinate resilience. We also proposed that the transactional leadership dimensions of Management-by-Exception Active and Management-by-Exception Passive and the non-leadership dimension of Laissez-Faire leadership would not be positively associated with subordinate resilience. With the exception of Inspirational Motivation, all hypothesized relationships were supported. A post-hoc analysis of open-ended responses to the question What helped you to deal with this situation? indicated that participants who mentioned their leaders as a positive factor in dealing with the situation exhibited greater resilience than participants who did not. The implications of these results and suggestions for future research are discussed