Three-component laser Doppler velocimeter measurements in a juncture flow

A single-axis, five-beam, three-component laser velocimeter (LV) system was used in a major experiment. Satisfactory results were obtained with the LV system in the juncture flow. Limited optical access to the tunnel proved to be a problem for the three component LV system in determining the third component

901-11 Late Clinical and Echocardiographic Follow-up After Left Ventricular Endoaneurysmorrhaphy

Infarct expansion and aneurysm (LVA) formation has a poor prognosis. Traditional techniques of LVA resection may be associated with suboptimal results, and do not fully restore LV geometry. LV endoaneurysmorrhaphy(LVEA) is a newer operative technique which utilizes an endocardial patch to exclude the aneurysm and normalize LV geometry. Late clinical and echocardiographic features of these patients (pts) is unknown. We prospectively followed 51 consecutive pts who had undergone LVEA. Average duration of follow-up (F/U) was 4.6 years (range 2-10 years). All pts had clinical evaluation and review of medical records.ResultsThere were 2 (4%) peri-operative deaths, 2 (4%) in-hospital deaths, and 13 (24%) late deaths. Clinical improvement was noted in all 34 survivors:NYHAClassPre-opF/UCCSPre-opF/Un(%)n(%)n(%)n(%)I5(15)21(62)I12(35)29(85)II9(26)8(24)II3(9)5(15)III13(38)4(12)III5(15)0IV7(21)1(3)IV14(41)030 surviving pts had F/U 2D echocardiograms (2DE). Near normal LV geometry was restored in all pts, and no patch aneurysms were noted at late F/U. 24/30 2DEs were adequate for quantitative analysis. The average LVEF post-op was 40.2% using the modified biplane analysis.ConclusionsLV endoaneurysmorrhaphy was associated with a 72% overall survival after average 4.6 year F/U. All survivors had improvement in clinical status and normalization of LV geometry

Stretch‐Induced Increase in Cardiac Contractility Is Independent of Myocyte Ca\u3csup\u3e2+\u3c/sup\u3e While Block of Stretch Channels by Streptomycin Improves Contractility After Ischemic Stunning

Stretching the cardiac left ventricle (LV) enhances contractility but its effect on myoplasmic [Ca2+] is controversial. We measured LV pressure (LVP) and [Ca2+] as a function of intra-LV stretch in guinea pig intact hearts before and after 15 min global stunning ± perfusion with streptomycin (STM), a stretch activated channel blocker. LV wall [Ca2+] was measured by indo-1 fluorescence and LVP by a saline-filled latex balloon inflated in 50 μL steps to stretch the LV. We implemented a mathematical model to interpret crossbridge dynamics and myofilament Ca2+ responsiveness from the instantaneous relationship between [Ca2+] and LVP ± stretching. We found that: (1) stretch enhanced LVP but not [Ca2+] before and after stunning in either control (CON) and STM groups, (2) after stunning [Ca2+] increased in both groups although higher in STM versus CON (56% vs. 39%), (3) STM-enhanced LVP after stunning compared to CON (98% vs. 76% of prestunning values), and (4) stretch-induced effects on LVP were independent of [Ca2+] before or after stunning in both groups. Mathematical modeling suggested: (1) cooperativity in cross-bridge kinetics and myofilament Ca2+ handling is reduced after stunning in the unstretched heart, (2) stunning results in depressed myofilament Ca2+ sensitivity in the presence of attached cross-bridges regardless of stretch, and (3) the initial mechanism responsible for increased contractility during stretch may be enhanced formation of cross-bridges. Thus stretch-induced enhancement of contractility is not due to increased [Ca2+], whereas enhanced contractility after stunning in STM versus CON hearts results from improved Ca2+ handling and/or enhanced actinomyosin cross-bridge cycling

Soluble ST2 levels and left ventricular structure and function in patients with metabolic syndrome

Background: A biomarker that is of great interest in relation to adverse cardiovascular events is soluble ST2 (sST2), a member of the interleukin family. Considering that metabolic syndrome (MetS) is accompanied by a proinflammatory state, we aimed to assess the relationship between sST2 and left ventricular (LV) structure and function in patients with MetS. Methods: A multicentric, cross-sectional study was conducted on180 MetS subjects with normal LV ejection fraction as determined by echocardiography. LV hypertrophy (LVH) was defined as an LV mass index greater than the gender-specific upper limit of normal as determined by echocardiography. LV diastolic dysfunction (DD) was assessed by pulse-wave and tissue Doppler imaging. sST2 was measured by using a quantitative monoclonal ELISA assay. Results: LV mass index (β=0.337, P<0 .001, linear regression) was independently associated with sST2 concentrations. Increased sST2 was associated with an increased likelihood of LVH [Exp (B)=2.20, P=0.048, logistic regression] and increased systolic blood pressure [Exp (B)=1.02, P=0.05, logistic regression]. Comparing mean sST2 concentrations (adjusted for age, body mass index, gender) between different LV remodeling patterns, we found the greatest sST2 level in the group with concentric hypertrophy. There were no differences in sST2 concentration between groups with and without LV DD. Conclusions: Increased sST2 concentration in patients with MetS was associated with a greater likelihood of exhibiting LVH. Our results suggest that inflammation could be one of the principal triggering mechanisms for LV remodeling in MetS

Population structure of Pentaclethra macroloba (Willd.) Kuntze in high and low floodplains of the Amazonian estuary.

The flood cycle of floodplain forests and topographic variations are important factors of diversification and morphological and ecophysiological adaptations of the plant community. Pentaclethra macroloba is a hyperdominant species adapted to flood variations. The objective of this study was to characterize the P. macroloba population structure between environments with high topography, flooded only during the rainy season and peak flood of the river, (high várzea-HV) and low topography with daily flood (low várzea-LV). The study was carried in the Mazagão Experimental Field, Brazil. Four plots of 1 ha were installed in each environment. All individuals with DBH &#8805; 5 cm were measured and georeferenced. ANOVA test, 0.05% probability, was applied to verify the structural differences between two environments. The HV had higher population density (51.2 individuals.ha-1) compared to the LV (36.7 individuals.ha-1), although without significance. Basal area was higher in the HV (10.42 m²) in comparison with LV (5.90 m²), with significant difference (F = 7.13, p = 0.00795). The maximum DAP of HV was 67 cm with mean of 24 cm. The LV presented maximum DBH of 42 cm with mean of 21 cm, showing structural difference between the two environments (F = 6.22, p = 0.0131). The aggregation index showed high aggregation in the LV (R = 0.89) than in the HV (R = 0.79), differing significantly between the two environments (F = 11.31, p = 0.0008). Frequency and time of flooding are ecological drives that structure the population of pracaxizeiro causing morphological and ecophysiological adaptations to the individual

The f-vector of the descent polytope

For a positive integer n and a subset S of [n-1], the descent polytope DP_S is the set of points x_1, ..., x_n in the n-dimensional unit cube [0,1]^n such that x_i >= x_{i+1} for i in S and x_i <= x_{i+1} otherwise. First, we express the f-vector of DP_S as a sum over all subsets of [n-1]. Second, we use certain factorizations of the associated word over a two-letter alphabet to describe the f-vector. We show that the f-vector is maximized when the set S is the alternating set {1,3,5, ...}. We derive a generating function for the f-polynomial F_S(t) of DP_S, written as a formal power series in two non-commuting variables with coefficients in Z[t]. We also obtain the generating function for the Ehrhart polynomials of the descent polytopes.Comment: 14 pages; to appear in Discrete & Computational Geometr

Serial magnetic resonance imaging based assessment of the early effects of an ACE inhibitor on postinfarction left ventricular remodeling in rats

In vivo assessment of treatment efficacy on postinfarct left ventricular (LV) remodeling is crucial for experimental studies. We examined the technical feasibility of serial magnetic resonance imaging (MRI) for monitoring early postinfarct remodeling in rats. MRI studies were performed with a 7-Tesla unit, 1, 3, 8, 15, and 30 days after myocardial infarction (MI) or sham operation, to measure LV mass, volume, and the ejection fraction (EF). Three groups of animals were analyzed: sham-operated rats (n = 6), MI rats receiving lisinopril (n = 11), and MI rats receiving placebo (n = 8). LV dilation occurred on day 3 in both MI groups. LV end-systolic and end-diastolic volumes were significantly lower in lisinopril-treated rats than in placebo-treated rats at days 15 and 30. EF was lower in both MI groups than in the sham group at all time points, and did not differ between the MI groups during follow-up. Less LV hypertrophy was observed in rats receiving lisinopril than in rats receiving placebo at days 15 and 30. We found acceptable within- and between-observer agreement and an excellent correlation between MRI and ex vivo LV mass (r = 0.96; p &lt; 0.001). We demonstrated the ability of MRI to detect the early beneficial impact of angiotensin-converting enzyme (ACE) inhibitors on LV remodeling. Accurate and noninvasive, MRI is the tool of choice to document response to treatment targeting postinfarction LV remodeling in rats