Isoflavonoids in non-leguminous families: an update

This review provides a listing of isoflavonoids reported in non-leguminous families. Reviews published to date have principally focused on plants with the richest isoflavonoid contents, the family Leguminosae. After a brief recall of the structure of isoflavonoids, we present all isoflavonoid structures encountered in non-leguminous families which may, thereby, become new plant sources for these compounds. Articles reporting on their different functions in plants are presented, as well as a brief summary discussing their potential benefits for human health. A list of 135 references is given

Isoflavonoids in non-leguminous families: an update

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Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis

Hepatitis C virus (HCV) is a unique enveloped virus that assembles as a hybrid lipoviral particle by tightly interacting with host lipoproteins. As a result, HCV virions display a characteristic low buoyant density and a deceiving coat, with host-derived apolipoproteins masking viral epitopes. We previously described methods to produce high-titer preparations of HCV particles with tagged envelope glycoproteins that enabled ultrastructural analysis of affinity-purified virions. Here, we performed proteomics studies of HCV isolated from culture media of infected hepatoma cells to define viral and host-encoded proteins associated with mature virions. Using two different affinity purification protocols, we detected four viral and 46 human cellular proteins specifically copurifying with extracellular HCV virions. We determined the C terminus of the mature capsid protein and reproducibly detected low levels of the viral nonstructural protein, NS3. Functional characterization of virion-associated host factors by RNAi identified cellular proteins with either proviral or antiviral roles. In particular, we discovered a novel interaction between HCV capsid protein and the nucleoporin Nup98 at cytosolic lipid droplets that is important for HCV propagation. These results provide the first comprehensive view to our knowledge of the protein composition of HCV and new insights into the complex virus–host interactions underlying HCV infection

RF Conditionning of the Spiral 2 CW RFQ

International audienceThe SPIRAL2 RFQ is designed to accelerate light and heavy ions with A/Q from 1 to 3 at 0.73 MeV/A. The nominal beam intensities are up to 5 mA CW for both proton and deuteron beams and up to 1 mA CW for heavier ions. The four-vane cavity is made with 5 1-meter long sections mechanically assembled, it works at 88 MHz and is powered up to 180 kW CW to achieve the nominal vane voltage of 113.7 kV for A/Q = 3 ions. This paper describes the RF conditioning of the RFQ at GANIL with the setting of its RF systems and cooling system used to tune the cavity resonance frequency