Measuring Exocytosis Rate Using Corrected Fluorescence Recovery After Photoconversion

Exocytosis plays crucial roles in regulating the distribution and function of plasma membrane (PM) and extracellular matrix proteins. However, measuring the exocytosis rate of a specific protein by conventional methods is very difficult because of exocytosis-independent trafficking such as endocytosis, which also affects membrane protein distribution. Here, we describe a novel method, corrected fluorescence recovery after photoconversion, in which exocytosis-dependent and -independent trafficking events are measured simultaneously to accurately determine exocytosis rate. In this method, the protein-of-interest is tagged with Dendra2, a green-to-red photoconvertible fluorescent protein. Following the photoconversion of PM-localized Dendra2, both the recovery of the green signal and the changes in the photoconverted red signal are measured, and the rate of exocytosis is calculated from the changing rates of these two signals

Continuum Coupling Effects in Spectra of Mirror Nuclei and Binding Systematics

Continuum-coupling correction to binding energies in neutron rich oxygen and fluorine isotopes and to excitation energies of 2+ states in 36Ca and 36S mirror nuclei are studied using the real-energy continuum shell model.Comment: Proc. XXX Mazurian Lakes Conference on Physics: Nuclear Physics and Fundamental Processes 15 pages, 5 figure

Intruder level and deformation in the SD-pair shell model

The influence of the intruder level on nuclear deformation is studied within the framework of the nucleon-pair shell model truncated to an SD-pair subspace. The results suggest that the intruder level has a tendency to soften the deformation and plays an important role in determining the onset of rotational behavior.Comment: 2 input TeX files, 2 figures, submitted to Phys. Lett.

Rotation and vibration in tetraquarks

A novel approach is introduced for obtaining precise solutions of the pairing Hamiltonian for tetraquarks, which utilizes an algebraic technique in infinite dimensions. The parameters involved in the transition phase are calibrated based on potential tetraquark candidates derived from phenomenology. Our investigation shows that the rotation and vibration transitional theory delivers a more accurate explanation for heavy tetraquarks compared to other methods utilizing the same formalism. To illustrate the concept, we compute the spectra of several tetraquarks, namely charm, bottom, bottom-charm and open charm and bottom systems, and contrast them with those of other particles.Comment: 13 pages, 4 figures, 4 Tables. Invited contribution to a Special Issue of Few Body Systems: "Emergence and Structure of Baryons -- Selected Contributions from the International Conference Baryons 2022

γ -rigid solution of the Bohr Hamiltonian for the critical point description of the spherical to γ -rigidly deformed shape phase transition

The γ-rigid solution of the Bohr Hamiltonian with the β-soft potential and 0 ≤γ≤30 is worked out. The resulting model, called T(4), provides a natural dynamical connection between the X(4) and the Z(4) critical-point symmetries, which thus serves as the critical-point symmetry of the spherical to γ-rigidly deformed shape phase transition. This point is further justified through comparing the model dynamics with those of the interacting boson model. As a preliminary test, the low-lying structures of Er158 are taken to compare the theoretical calculations, and the results indicate that this nucleus could be considered as the candidate of the T(4) model with an intermediate γ deformation

Double-Carrier Phase-Disposition Pulse Width Modulation Method for Modular Multilevel Converters

Modular multilevel converters (MMCs) have become one of the most attractive topologies for high-voltage and high-power applications. A double-carrier phase disposition pulse width modulation (DCPDPWM) method for MMCs is proposed in this paper. Only double triangular carriers with displacement angle are needed for DCPDPWM, one carrier for the upper arm and the other for the lower arm. Then, the theoretical analysis of DCPDPWM for MMCs is presented by using a double Fourier integral analysis method, and the Fourier series expression of phase voltage, line-to-line voltage and circulating current are deduced. Moreover, the impact of carrier displacement angle between the upper and lower arm on harmonic characteristics is revealed, and further the optimum displacement angles are specified for the circulating current harmonics cancellation scheme and output voltage harmonics minimization scheme. Finally, the proposed method and theoretical analysis are verified by simulation and experimental results

Analytically solvable prolate-oblate shape phase transitional description within the SU(3) limit of the interacting boson model

A novel analytically solvable prolate-oblate shape phase transitional description for the SU(3) limit of the interacting boson model is investigated for finite-N as well as in the large-N classical limit. It is shown that the ground state shape phase transition is of first order due to level crossing. Through a comparison of the theoretical predictions with available experimental data for even-even 180Hf, 182-186W, 188-190Os, and 192-198Pt, it is shown that this simple novel description is suitable for a description of the prolate-oblate shape phase transition in these nuclei. © 2012 American Physical Society

Simple description of odd-A nuclei around the critical point of the spherical to axially deformed shape phase transition

An analytically solvable model, X(3/2j+1), is proposed to describe odd-A nuclei near the X(3) critical point. The model is constructed based on a collective core described by the X(3) critical point symmetry coupled to a spin-j particle. A detailed analysis of the spectral patterns for cases j=1/2 and j=3/2 is provided to illustrate dynamical features of the model. By comparing theory with experimental data and results of other models, it is found that the X(3/2j+1) model can be taken as a simple yet very effective scheme to describe those odd-A nuclei with an even-even core at the critical point of the spherical to axially deformed shape phase transition. © 2011 American Physical Society