11 research outputs found
Construction and Synergistic Effect of Recombinant Yeast Co-expressing Pig IL-2/4/6 on Immunity of Piglets to PRRS Vaccination
AbstractIn order to develop cost-effective immunomodulator, the recombinant Pichia pastoris were firstly constructed to co-express porcine IL-2/4/6 genes, and then fermented to feed 45-days Tibetan piglets at different doses to evaluate its effects on immunity of piglets to PRRS vaccination, which simultaneously received intramuscular injection of inactivated PRRS vaccine. The results were found that the leukocytes, IgG and specific antibody to PRRSV, Th and Tc cells increased significantly in the blood of treated piglets in comparison with those of the control (P<0.05); the mRNA expression of TLRs (TLR-2, 3, 4, 7, 9), IFN-γ, IL-2, IL-4, IL-6, IL-7, IL-12 and IL-15 genes were elevated significantly in the immune cells from the blood of treated piglets (P<0.05). Moreover, the growth of the treated piglets also markedly improved whose average net weight gain was significantly higher than the control on 58 days post inoculation (P<0.05). These results suggest that the recombinant yeast can effectively enhance the systematic innate and adaptive immunity of piglets as well as promote the growth of piglet, which could be further developed as cost-effective promising immunomodulator to improve the control of pig PRRS disease
Theoretical Kinetic Studies on Thermal Decomposition of Glycerol Trinitrate and Trimethylolethane Trinitrate in the Gas and Liquid Phases
Glycerol trinitrate (NG) and trimethylolethane trinitrate
(TMETN),
as typical nitrate esters, are important energetic plasticizers in
solid propellants. With the aid of high-precision quantum chemical
calculations, the Rice-Ramsperger-Kassel-Marcus (RRKM)/master equation
theory and the transition state theory have been employed to investigate
the decomposition kinetics of NG and TMETN in the gas phase (over
the temperature range of 300–1000 K and pressure range of 0.01–100
atm) and liquid phase (using water as the solvent). The continuum
solvation model based on solute electron density (SMD) was used to
describe the solvent effect. The thermal decomposition mechanism is
closely relevant to the combustion properties of energetic materials.
The results show that the RO–NO2 dissociation channel
overwhelmingly favors other reaction pathways, including HONO elimination
for the decomposition of NG and TMETN in both the gas phase and liquid
phase. At 500 K and 1 atm, the rate coefficient of gas phase decomposition
of TMETN is 5 times higher than that of NG. Nevertheless, the liquid
phase decomposition of TMETN is a factor of 5835 slower than that
of NG at 500 K. The solvation effect caused by vapor pressure and
solubility can be used to justify such contradictions. Our calculations
provide detailed mechanistic evidence for the initial kinetics of
nitrate ester decomposition in both the gas phase and liquid phase,
which is particularly valuable for understanding the multiphase decomposition
behavior and building detailed kinetic models for nitrate ester
Construction and Synergistic Effect of Recombinant Yeast Co-expressing Pig IL-2/4/6 on Immunity of Piglets to PRRS Vaccination
Should Small, Young Technology-Based Firms Internalize Transactions in Their Internationalization?
The belt and road initiative, cultural friction and ethnicity: Their effects on the export performance of SMEs in China
β-Delayed γ Emissions of 26P and Its Mirror Asymmetry
The study of the origin of asymmetries in mirror β decay is extremely important to understand the fundamental nuclear force and the nuclear structure. The experiment was performed at the National Laboratory of Heavy Ion Research Facility in Lanzhou (HIRFL) to measure the β-delayed γ rays of 26P by silicon array and Clover-type high-purity Germanium (HPGe) detectors. Combining with results from the β decay of 26P and its mirror nucleus 26Na, the mirror asymmetry parameter δ ( ≡ft+/ft−− 1) was determined to be 46(13)% for the transition feeding the first excited state in the daughter nucleus. Our independent results support the conclusion that the large mirror asymmetry is close to the proton halo structure in 26P
<i>β</i>-Delayed <i>γ</i> Emissions of <sup>26</sup>P and Its Mirror Asymmetry
The study of the origin of asymmetries in mirror β decay is extremely important to understand the fundamental nuclear force and the nuclear structure. The experiment was performed at the National Laboratory of Heavy Ion Research Facility in Lanzhou (HIRFL) to measure the β-delayed γ rays of 26P by silicon array and Clover-type high-purity Germanium (HPGe) detectors. Combining with results from the β decay of 26P and its mirror nucleus 26Na, the mirror asymmetry parameter δ ( ≡ft+/ft−− 1) was determined to be 46(13)% for the transition feeding the first excited state in the daughter nucleus. Our independent results support the conclusion that the large mirror asymmetry is close to the proton halo structure in 26P