Правовые аспекты, касающиеся пищевых добавок в Румынии и Европейском Союзе

Disciplina Biologie vegetală şi animală (I), Facultatea de Farmacie, UMF Grigore T. Popa, Iaşi, România, Disciplina Analiza medicamentului, Legislaţie şi Deontologie Farmaceutică, Facultatea de Farmacie, UMF Grigore T. Popa, Iaşi, România, Conferința științifico-practică cu participare internațională Centrul de Sănătate Publică din municipiul Chișinău – 70 de ani la straja sănătăţii 23 octombrie 2014This paper aims to describe the most important legislative regulations on food supplements in Romania and the European Union. Pending the adoption of common legislation in all countries of the Union, each Member State shall develop its own rules in order to control and provide safety in the administration of supplements. These include directives on procedures for authorization, producer responsibility, quality control and labelingВ статье излагаются наиболее важные законодательные акты, касающиеся пищевых добавок в Румынии и Европейском Союзе. До принятия общего для всех стран законодательства, каждое государство разрабатывает свои собственные правила с целью контроля и обеспечения безопасности при реализации добавок. Они включают директивы, касающиеся методов авторизации, ответственности производителей, контроля качества и маркировки

Application of infrared Thermography in rabbit orthopaedic models

The rectal or internal temperature (BT) is a reference method for body temperature. BT and ear temperature (BTear) were recorded in rabbit orthopaedic experimental model - White New Zeeland rabbits (N = 14), for a six day post-surgery period. Ear (BTear) temperature measured with infrared thermography (IRT) camera was compared with rectal body temperature (BT) measured with digital thermometer. Each BTear and BT methods were studied by analysis of variance and for BT classes such as: hypothermia (BTh≥ than 38,5оC), normothermia (BTn) and hyperthermia or fever (BTf ≥ 40,0 оC). Mean differences, linear regression and Pearson correlation were analysed. BTear was positively correlated with rectal temperature (BT); r=+0.579 at p <0.001. The regression equation model was statistically acceptable (p<0.001) and value of internal body temperature can be estimated on ITR measurements by relation: BT (оC) = 25.498 + BTear x 0.361 with R2=0.336. This study demonstrates that IRT technology, a passive and non-contact technology can be effectively used for estimating BT changes in rabbits

Retrospective analysis on somatic cells count trend in Staphylococcus aureus cows' mastitis

cells count (SCC) of two successive samplings. The analysis was performed to identify the associations between SCC trend with retrospective and transversal milk quality indicators, and mastitis. From a BIOAMR database, 28 cows with a posteriori diagnosed mastitis with Staphylococcus aureus (4/28 cases were methicillin-resistant Staphylococcus aureus – MRSA) were sampled. The trend of SCC was Spearman's rho correlated with previous lactose (rs = +0.785, p=0.03), pH (rs = +0.662 at p=0.019), and current SCC measurements (rs = +0.781, at p=0.000). Increasing trend of SCC was retrospectively associated with lactose content (Z = -2.152 at p = 0.031), pH (Z = -2.152 at p = 0.031) SCC at first measurement (Z = - 1.764 at p = 0.078) and currently associated with SCC (Z = -3.316 at p = 0.001), fat content (Z = -1.88 at p = 0.060) and fat/protein ratio (Z = -1.717 at p = 0.086). The 28 samples of the study did not revealed strong association between SCC trend and type of Staphylococcus aureus (p = 0.186 by Mann-Whitney test), even if MRSA had a higher increasing trend of SCC in comparison whit S. aureus (non-MRSA) infections (1403.5 vs. 288.2 thousands somatic cells). By preliminary results the trend of somatic cells could be an indicator in detection of mastitis but more case studies are necessary

In vitro bovine embryos evaluation based on OCT4, SOX2, IGF1R and IGF2R expression level

In vitro production of bovine embryos comprises a lot of factors that can influence the successful of this technique, oxidative stress being one of them. These factors can influence the evolution of important development processes such as the maternal to zygotic transition and the embryonic genome activation. Adding antioxidants to in vitro culture media exerts the key role to reduce the effects of reactive oxidative species produced during assisted reproduction technique, influencing in a positive way also the early embryonic developement. The objective of this study was to determine the effect of antioxidant rosmarinic acid (105 μM), added to in vitro bovine oocytes maturation media, on the quality of embryo produced based on gene expression level of OCT4, SOX2, IGF1R and IGF2R. For this purpose, we used 35 bovine ovaries taken from slougtherhouse from which we obtain 202 cumulus-oocyte-complexes and 127 of them were maturated in vitro based on morphological aspects. The cumulus-oocyte-complexes were divided in two groups: control (M1, M2, M3) and with acid rozmarinic (AR1, AR2 and AR3). The levels of OCT4, SOX2, IGF1R and IGF2R were the highest in group AR1, embryos obtain from oocytes class I supplemented with rozmarinic acid, where OCT4 expression was 4.08, SOX2 was 27.66, IGF1R and IGF2R were 53.44 and 25.10

Collection of blastomeres in order to establish sex and isolate genetic material-review

Globally, limited access to food needs in relation to meat or milk production has required the establishment of the sex of offspring from the embryonic stage. While the meat industry uses males, the dairy industry relies on females. During the period of exploitation, the number of products obtained from a female bovine is 5-6 individuals, their sex being able to be influenced by means of sexed semen. Embryo sexing programs can result in a large number of conception products, in a shorter period of time taking into account the desired sex. The use of the desired sex embryo facilitates the improvement of the genetic value. Embryonic sexing procedures involve the collection by biopsy of a minimum amount of genetic material that can ensure the determination of sex. Both invasive and non-invasive biopsy and sexing procedures can influence the subsequent viability of embryos prepared for embryo transfer. This paper highlights the methods of embryonic sexing along with the advantages and disadvantages of each technique involved in determining sex

Unusual Canine Distemper Virus Infection in Captive Raccoons (Procyon lotor)

Canine morbillivirus, also known as canine distemper virus (CDV), is the causative agent of canine distemper (CD), which is a serious contagious disease of canines, large felids, and, occasionally, raccoons. This study included seven raccoons from the Timisoara Zoological Garden, Romania. CDV was detected using RT-qPCR on blood samples, but several other exams were also performed—clinical, bacteriological, immunohistochemistry (IHC) and histopathology, toxicological screening, and necropsy—which confirmed CDV infection. Severe digestive disorders (diarrhea and frequent hematemesis) were observed. The necropsy findings included pseudo membranous gastroenteritis, congestion, and pulmonary edema in two raccoons. Immunohistochemistry showed immunolabeled CDV antigenantibodies on the viral nucleocapsid. Histopathology revealed lymphocyte depletion in mesenteric lymphnodes and intranuclear and intracytoplasmic inclusions in the enterocytes of the small intestine. Based on the RT-qPCR assay, laboratory tests, and the lesions observed, it was established that the raccoons were infected with CDV, which was the cause of death in two cases. The results from the necropsy, histology, and immunohistochemistry in the raccoons are comparable with reported CDV lesions in dogs. In conclusion, several exams may be performed to establish the etiology of possible interspecific viral infection, but only very specific exams can identify aCDV infection. Laboratory analyses must be completed by RT-qPCR assay or IHC to establish infection with uncommon viruses in raccoons with high accuracy