Sex-differences in the longitudinal recovery of neuromuscular function in COVID-19 associated acute respiratory distress syndrome survivors

Introduction: Patients admitted to the intensive care unit (ICU) following severe acute respiratory syndrome 2 (SARS-CoV-2) infection may have muscle weakness up to 1 year or more following ICU discharge. However, females show greater muscle weakness than males, indicating greater neuromuscular impairment. The objective of this work was to assess sex differences in longitudinal physical functioning following ICU discharge for SARS-CoV-2 infection. Methods: We performed longitudinal assessment of physical functioning in two groups: 14 participants (7 males, 7 females) in the 3-to-6 month and 28 participants (14 males, 14 females) in the 6-to-12 month group following ICU discharge and assessed differences between the sexes. We examined self-reported fatigue, physical functioning, compound muscle action potential (CMAP) amplitude, maximal strength, and the neural drive to the tibialis anterior muscle. Results: We found no sex differences in the assessed parameters in the 3-to-6-month follow-up, indicating significant weakness in both sexes. Sex differences emerged in the 6-to-12-month follow-up. Specifically, females exhibited greater impairments in physical functioning, including lower strength, walking lower distances, and high neural input even 1 year following ICU-discharge. Discussion: Females infected by SARS-CoV-2 display significant impairments in functional recovery up to 1 year following ICU discharge. The effects of sex should be considered in post-COVID neurorehabilitation

Oral diagnosis and treatment planning: Part 6. Preventive and treatment planning for periodontal disease

A high level of sustained personal plaque control is fundamental for successful treatment outcomes in patients with active periodontal disease and, hence, oral hygiene instructions are the cornerstone of periodontal treatment planning. Other risk factors for periodontal disease also should be identified and modified where possible. Many restorative dental treatments in particular require the establishment of healthy periodontal tissues for their clinical success. Failure by patients to control dental plaque because of inappropriate designs and materials for restorations and prostheses will result in the long-term failure of the restorations and the loss of supporting tissues. Periodontal treatment planning considerations are also very relevant to endodontic, orthodontic and osseointegrated dental implant conditions and proposed therapies.E. Corbet and R. Smale

Listeria Membrane Protrusion Collapse: Requirement of Cyclophilin A for Listeria Cell-to-Cell Spreading

Listeria generate actin-rich tubular protrusions at the plasma membrane that propel the bacteria into neighboring cells. The precise molecular mechanisms governing the formation of these protrusions remain poorly defined. In this study, we demonstrate that the prolyl cis-trans isomerase (PPIase) cyclophilin A (CypA) is hijacked by Listeria at membrane protrusions used for cell-to-cell spreading. Cyclophilin A localizes within the F-actin of these structures and is crucial for their proper formation, as cells depleted of CypA have extended actin-rich structures that are misshaped and are collapsed due to changes within the F-actin network. The lack of structural integrity within the Listeria membrane protrusions hampers the microbes from spreading from CypA null cells. Our results demonstrate a crucial role for CypA during Listeria infections

Consensus Report : 2nd European Workshop on Tobacco Use Prevention and Cessation for Oral Health Professionals

Tobacco use has been identified as a major risk factor for oral disorders such as cancer and periodontal disease. Tobacco use cessation (TUC) is associated with the potential for reversal of precancer, enhanced outcomes following periodontal treatment, and better periodontal status compared to patients who continue to smoke. Consequently, helping tobacco users to quit has become a part of both the responsibility of oral health professionals and the general practice of dentistry. TUC should consist of behavioural support, and if accompanied by pharmacotherapy, is more likely to be successful. It is widely accepted that appropriate compensation of TUC counselling would give oral health professionals greater incentives to provide these measures. Therefore, TUC-related compensation should be made accessible to all dental professionals and be in appropriate relation to other therapeutic interventions. International and national associations for oral health professionals are urged to act as advocates to promote population, community and individual initiatives in support of tobacco use prevention and cessation (TUPAC) counselling, including integration in undergraduate and graduate dental curricula. In order to facilitate the adoption of TUPAC strategies by oral health professionals, we propose a level of care model which includes 1) basic care: brief interventions for all patients in the dental practice to identify tobacco users, assess readiness to quit, and request permission to re-address at a subsequent visit, 2) intermediate care: interventions consisting of (brief) motivational interviewing sessions to build on readiness to quit, enlist resources to support change, and to include cessation medications, and 3) advanced care: intensive interventions to develop a detailed quit plan including the use of suitable pharmacotherapy. To ensure that the delivery of effective TUC becomes part of standard care, continuing education courses and updates should be implemented and offered to all oral health professionals on a regular basis

Implant dentistry in undergraduate dental curricula in South-East Asia: forum workshop at the University of Hong Kong, Prince Philip Dental Hospital, 19–20 November 2010

This paper reports on the discussions arising from a 2-day forum on implant dentistry education in South-East Asia. The 10 institutions present represented undergraduate and postgraduate dental curricula from seven countries, including Hong Kong, Indonesia, Malaysia, Taiwan, Thailand, the Philippines, and Singapore. While not aiming to reach consensus as in other such conferences, the outcome was positive in establishing realistic goals in university education in implant dentistry for curriculum leaders and developers

Effectiveness of different interdental brushes on cleaning the interproximal surfaces of teeth and implants: a randomized controlled, double-blind cross-over study

OBJECTIVES: To compare the interproximal cleansing efficacy of the novel, waist-shaped Circum brush (Topcaredent, Switzerland; CB) with that of a straight soft interdental brush (IB) (TePe, Sweden; SB) on posterior surfaces. MATERIAL and METHODS: Eight patients after completion of initial periodontal therapy abolished oral hygiene for 3 days. Baseline plaque scores (PlI, Silness and Loe ) were assessed on eight surfaces of all premolars and molars. Subsequently, an instructed nurse applied at random one of the two IB, three times per interdental space . Following this, registration of the PlI was repeated by the same blinded examiner. After a 2-week recovery, patients abolished oral hygiene practices for another 3 days. Again, pre-and post-brushing PlI were recorded by the same examiner. The second IB was now applied. RESULTS: Patient mean PlI and site PlI were evaluated before and after application of the SB or CB respectively. Paired t-tests were performed to yield statistically significant differences. The reduction of biofilm from before to after the use of the IB on a subject basis was highly significant (P < 0.0001). The mean PlI after the use of the CB was significantly lower than after the use of the SB (P < 0.0001). Comparing the PlI of the line angles (MB, ML, DB, DL), significantly more biofilm had been removed by applying CB compared with SB (P < 0.0001). Moreover, comparing the PlI of the buccal (MB, DB) or the lingual line angles (DL, ML) yielded a significantly higher reduction of biofilm in favour of the CB (P < 0.0001). The reduction of the PlI in the mid-interproximal portion, both mesially and distally did not differ significantly between CB and ST. No biofilm reduction was seen on the buccal sites with either IB. CONCLUSION: The application of the waist-shaped Circum IB resulted in significantly lower PlI scores than the use of a straight IB. This was predominantly due to the higher cleansing effect of the waist-shaped CB on the buccal and lingual line angles.link_to_subscribed_fulltex

Factors influencing resonance frequency analysis assessed by Osstell™ mentor during implant tissue integration: II. Implant surface modifications and implant diameter

Objectives:: To monitor the development of the stability of Straumann ® tissue-level implants during the early phases of healing by resonance frequency analysis (RFA) and to determine the influence of implant surface modification and diameter. Material and methods:: A total of twenty-five 10 mm length implants including 12 SLA RN ∅4.1 mm implants, eight SLActive RN ∅4.1 mm implants and five SLA WN ∅4.8 mm implants were placed. Implant stability quotient (ISQ) values were determined with Osstell ™ mentor at baseline, 4 days, 1, 2, 3, 4, 6, 8 and 12 weeks post-surgery. ISQ values were compared between implant types using unpaired t-tests and longitudinally within implant types using paired t-tests. Results:: During healing, ISQ decreased by 3-4 values after installation and reached the lowest values at 3 weeks. Following this, the ISQ values increased steadily for all implants and up to 12 weeks. No significant differences were noted over time. The longitudinal changes in the ISQ values showed the same patterns for SLA implants, SLActive implants and WB implants. At placement, the mean ISQ values were 72.6, 75.7 and 74.4, respectively. The mean lowest ISQ values, recorded at 3 weeks, were 69.9, 71.4 and 69.8, respectively. At 12 weeks, the mean ISQ values were 76.5, 78.8 and 77.8, respectively. The mean ISQ values at all observation periods did not differ significantly among the various types. Single ISQ values ranged from 55 to 84 during the entire healing period. Pocket probing depths of the implants ranged from 1 to 3 mm and bleeding on probing from 0 to 2 sites/implant post-surgically. Conclusions:: All ISQ values indicated the stability of Straumann ® implants over a 12-week healing period. All implants showed a slight decrease after installation, with the lowest ISQ values being reached at 3 weeks. ISQ values were restored 8 weeks post-surgically. It is recommended to monitor implant stability by RFA at 3 and 8 weeks post-surgically. However, neither implant surface modifications (SLActive) nor implant diameter were revealed by RFA. © 2010 John Wiley & Sons A/S.link_to_subscribed_fulltex

Ante's (1926) law revisited: A systematic review on survival rates and complications of fixed dental prostheses (FDPs) on severely reduced periodontal tissue support

Background: In subjects suffering from generalized severe periodontitis, only a few teeth may be treated and used as abutments for fixed dental prostheses (FDPs). Objective: To systematically review the impact of severely reduced, but healthy periodontal tissue support on the survival rate and complications of FDPs after a mean follow-up time of at least 5 years. Search strategy: Publications considered for inclusion were searched in MEDLINE (PubMed) and relevant journals were hand searched. The search was performed in duplicate and was limited to human studies published in the dental literature from 1966 up to and including September 2006. Only publications in English, in peer-reviewed journals, were considered. Abstracts were excluded. Selection criteria: Prospective and retrospective cohort studies were included. The primary outcome measure included survival rates of FDPs and abutment teeth, whereas biological and technical complications of FDPs and abutment teeth represented secondary outcome measures. Data analysis: Summary estimates of survival rates and of biological and technical complications were calculated after 5 and 10 years. Results: The search provided 860 titles of which six publications were included. A total of 579 FDPs were incorporated and followed up to 25 years. Meta-analysis yielded an estimated FDP survival rate of 96.4% [95% confidence interval (95% CI): 94.6-97.6%] after five and of 92.9% (95% CI: 89.5-95.3%) after 10 years, respectively. After 10 years, the estimated rate of abutment teeth without endodontic complications amounted to 93% (95% CI: 62.6-98.9%). The 10-year estimated rate of caries-free abutment teeth was 98.1% (95% CI: 88.2-99.7%). FDPs without loss of retention were estimated to occur in 95.4% (95% CI: 92.6-97.2%) of cases after 10 years. Conclusions: These results showed that (i) masticatory function could be established and maintained in subjects receiving FDPs on abutment teeth with severely reduced but healthy periodontal tissue support and (ii) FDPs survival rates compared favourably with those of FDPs incorporated in subjects without severely periodontally compromised dentitions. © 2007 Blackwell Munksgaard.link_to_subscribed_fulltex

Nd:YAG (1064 nm) laser for the treatment of chronic periodontitis: a pilot study

AIM: To evaluate the clinical and microbiological effects of neodymium: yttrium-aluminum-garnet laser therapy as an adjunct to scaling and root planing during the hygienic phase. METHODS: In eight patients, sites with a mean probing pocket depth (PPD) of ≥5 mm were treated by either scaling and root planing (n=28) (control) or by scaling and root planing and adjunctive laser therapy (n=28) (power: 5W). Re-evaluation was at 4-6 weeks. Thereafter, remaining pockets (mean PPD ≥5 mm) were eliminated by either laser surgery (power: 7 W) or gingivectomy (control). RESULTS: At baseline, the mean PPD of sites originally presenting with a mean PPD ≥4 mm were 4.69 and 4.73 mm in the test and control sites, respectively. Six months following surgery, there was a similar average mean PPD reduction in the test (1.18 mm, P<0.01) and control sites (1.35 mm, P<0.01). Also, the reduction in bleeding on probing in both groups was statistically significant (P<0.01, paired t-tests). No statistically-significant differences between the test and control sites were found for any clinical or microbiological parameters at baseline, after initial, and 3 or 6 months' post-surgical therapy. CONCLUSION: During the hygienic phase, neodymium: yttrium-aluminum-garnet (1064 nm) laser treatment yielded no superiority in clinical efficacy compared to conventional debridement. Laser gingivectomy resulted in similar treatment outcomes (mean PPD and bleeding on probing reduction), as did conventional gingivectomy