Seismic Refraction Method

Seismic methods are applied primarily in order to determine quasi-homogeneous zonesaccording to parameters of fragmentation, physical and chemical weathering anddeformability of rock masses and cohesionless soil. Applied seismic methods comprisesending impulses underground and registering the resulting refracted arrivals from subsurfaceinterfaces on a number of receivers positioned on or near the surface. Times elapsed fromsending to receiving seismic waves depend on depths of studied structures and propagationvelocities of seismic waves along paths of their propagation from the source to the refractor(or reflector) to the receiver.This paper presents two examples of preparation of seismic sections as a basis forgeotechnical design of foundations. Both examples are presentations of an optimum programof exploratory boring supplemented by results of deep seismic refraction studies.Results of geophysical investigations should be included in the data obtained bygeological mapping, which will in correlation with drilling results complete the picture ofgeological structure of terrain and facilitate categorization of materials and rocks for thepurpose of developing the engineering and hydrogeological profile as a base for designers inthe project execution stage

Reducing the Sodium Chloride Content in Chicken Pate by Using Potassium and Ammonium Chloride

AbstractThe aim of this research was to investigate possibility of chicken pate production with reduced sodium chloride content, as well as to establish changes in sensory characteristics. In the study, six experimental groups of chicken pate were produced with the same basic ingredients, but different amounts of added salts. Sensory evaluation was performed in order to determine general taste acceptability, and of the sodium and potassium levels in the chicken pate. The pate from EI and EII groups in which the amount of added sodium chloride was reduced and/or partially substituted with ammonium chloride had a most acceptable taste

Pomološke i proizvodne osobine nekih novijih sorti šljive

Plum assortment in Serbia is dominated by old autochthonous cultivars, well-known cultivar ‘Stanley’ and cultivars named and released in Fruit Research Institute, Čačak such as: ‘Čačanska lepotica’, ‘Čačanska rodna’, ‘Čačanska rana’ and ‘Čačanska najbolja’. In order to fulfill different demands of fresh fruit market and achieve better results in modern plum production, it is necessary, among other things, to introduce new cultivars. Before introduction of new cultivars in orchards, significant evaluation of these cultivars in environmental conditions of region they are being introduced is required. The main objective of this study was to evaluate the most importand pomological and productive traits of six introduced plum cultivars: ‘Hanita’, ‘Jojo’, ‘Jubileum’, ‘Presenta’, ‘Tegera’ and ‘Valor’. The most important phenological (flowering and ripening time), and morphometric traits (fruit and stone weight, fruit dimensions and flash percentage), as well as tree vigour (trunk cross section area) and cropping potential (yield per tree and yield efficiency) were evaluated. Flowering phenofase in all examined cultivars lasted during the second decade of april, whereby flowering abundance was estimated as very good. The largest fruit weight, and fruit dimensions (height, width and thickness) were determined in ‘Valor’, whereas the largest stone weight and tree vigour were determined in ‘Jubileum’. The largest flesh percentage was found in cultivars ‘Jojo’, ‘Tegera’ and ‘Valor’. Cultivars ‘Presenta’ and ‘Jojo’ had largest yield per tree, while ‘Jojo’ had the highest yield efficiency.U sortimentu šljive u Srbiji dominiraju stare, authtone sorte, dobro poznata sorta ‘Stanley’ i sorte stvorene u Institutu za voćarstvo, Čačak, pre svih ‘Čačanska lepotica’, ‘Čačanska rodna’, ‘Čačanska rana’ i ‘Čačanska najbolja’. Da bi se zadovoljili različiti zahtevi tržišta i postigli što bolji rezultati u savremenoj proizvodnji šljive, neophodno je, između ostalog, introdukovati nove sorte. Pre uvođenja u proizvodnju, ove sorte zahtevaju značajno ispitivanje u ekološkim uslovima regiona u koji se introdukuju. Cilj ovog rada je bio da se ispitaju najznačajnije pomološke i proizvodne osobine šest introdukovanih sorti šljive: ‘Hanita’, ‘Jojo’, ‘Jubileum’, ‘Presenta’, ‘Tegera’ i ‘Valor’. Ispitivane su najznačajnije fenološke (fenofaza cvetanja i sazrevanja plodova) i morfometrijske osobine (masa i dimenzije ploda, masa koštice i randman mezokarpa ploda), kao i bujnost (površina poprečnog preseka debla) i rodnost (prinos po stablu i koeficijent rodnosti). Fenofaza cvetanja je kod svih ispitivanih sorti trajala tokom druge dekade aprila u obe ispitivane godine, pri čemu je obilnost cvetanja ocenjena kao veoma dobra. Najveća masa, visina, širina i debljina ploda su utvrđeni kod sorte ‘Valor’, dok su najveća masa koštice i bujnost stabla utvrđeni kod sorte ‘Jubileum’. Najveći randman ploda imale su sorte ‘Jojo’, ‘Tegera’ i ‘Valor’. Sorte ‘Presenta’ i ‘Jojo’ su imale najveći prinos po stablu, dok je sorta ‘Jojo’ imala i najveći koeficijent rodnosti

Biološko-pomološke osobine autohtonih sorti šljive u agroekološkim uslovima Čačka

This study was carried out to determine basic biological and pomological traits of eight ex situ autochthonous plum cultivars (‘Belošljiva Ljubić’, ‘Bugarka’, ‘Cerovački piskavac’, ‘Crnošljiva’, ‘Crvena ranka’, ‘Dragačevka’, ‘Papračanka’ and ‘Petrovača’) during two consecutive years (2015 and 2016). Examination included investigation of the main phenological properties (flowering and ripening time), morphometrical (fruit and stone weight and flesh percentage) and sensorial properties of fruit (fruit scin and flesh colour, flesh firmness and stone adherence), as well as vigour (trunk cross sectional area) and cropping potential (yield per tree and yield efficiency). Studied plum cultivars flowered from the end of the first to the mid of the third decade of April and ripened during the third decade of July and the first and the second decade of August. The highest vigour and yield per tree was found in cultivar ‘Dragačevka’, while the highest yield efficiency was observed in cultivar ‘Cerovački piskavac’. Cultivar ‘Belošljiva Ljubić’ had the highest values of fruit and stone weight. The highest velue of flesh percentage was observed in cultivar ‘Dragačevka’. Dark-blue fruit skin colour was dominant, while flesh colour varied from yellowish green to yellow. Flesh was medium firm to firm and non adherent, semi adherent or adherent to the stone.Ispitivanja obuhvaćena ovim radom su sprovedena sa ciljem determinacije najvažnijih biološko-pomoloških osobina osam autohtonih sorti šljive (Belošljiva Ljubić, Bugarka, Cerovački piskavac, Crnošljiva, Crvena ranka, Dragačevka, Papračanka i Petrovača) u ex situ uslovima tokom 2015. i 2016. godine. Proučavane su najznačajnije fenološke osobine (fenofaze cvetanja i sazrevanja plodova), morfometrijske (masa ploda i koštice, randman mezokarpa ploda) i senzorne osobine ploda (boja pokožice i mezokarpa ploda, čvrstina mezokarpa i stepen prijanjanja koštice), kao i bujnost (površina poprečnog preseka debla) i rodnost (prinos po stablu i koeficijent rodnosti). Proučavani genotipovi šljive su cvetali od kraja prve do sredine treće dekade aprila, a sazrevali tokom treće dekade jula i prve i druge dekade avgusta. Najvećom bujnošću i najvišim prinosom po stablu se odlikovala sorta Dragačevka, dok je najviši indeks rodnosti utvrđen kod sorte Cerovački piskavac. Najveća masa ploda i koštice su utvrđene kod sorte Belošljiva Ljubić, a najveći randman mezokarpa ploda kod sorte Dragačevka. Dominirala je tamno plava boja pokožice ploda. Mezokarp ploda je bio žute ili žuto-zelene boje, srednje čvrst do čvrst, dok je koštica bila slobodna, delimično slobodna ili je u potpunosti prijanjala za mezokarp ploda

Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products

Soj bakterije Lactobacillus helveticus BGRA43, izoliran iz crijeva čovjeka, ima antimikrobni učinak na sojeve patogenih mikroorganizama u hrani, a tijekom fermentacije u mlijeku oslobađa peptide protuupalnih svojstava. U ovom je radu potvrđeno da soj BGRA43 djeluje antimikrobno na patogene sojeve, kao što su: Yersinia enterocolitica, Shigella sonnei, S. flexneri i Streptococcus pneumoniae. Stanice soja BGRA43 mogu preživjeti u simuliranim uvjetima gastrointestinalnog sustava uz dodatak mlijeka, pri čemu zadržavaju dovoljnu brojnost. Osim toga, LC/MS/MS analizom potvrđeno je da stanice soja BGRA43 mogu hidrolizirati β-laktoglobulin. U prisutnosti prebiotika, tj. inulina ili koncentrata β-glukana iz zobenih mekinja (Nutrim®) povećao se rast stanica soja BGRA43, čak i kad je prebiotik upotrijebljen kao jedini izvor ugljika. Soj BGRA43 odlično je rastao i u punomasnom kravljem ili kozjem mlijeku, te mlijeku koje je sadržavalo inulin ili Nutrim. Primjenom probiotičkog soja BGRA43 kao starter kulture dobiveni su fermentirani mliječni proizvodi od kravljeg ili kozjeg mlijeka sa ili bez dodatka inulina ili Nutrima s otprilike 107 CFU/mL stanica probiotika. Svi su proizvodi bili homogeni i viskozni, a najbolju su senzorsku ocijenu dobili fermentirani napici proizvedeni od rekonstituiranog mlijeka, punomasnog kravljeg mlijeka te kozjeg mlijeka s dodatkom 1 % inulina.Lactobacillus helveticus BGRA43 isolated from human intestines shows antimicrobial activity against foodborne pathogens and during fermentation in milk releases peptides with demonstrated anti-inflammatory properties. In this study, it was found that strain BGRA43 exhibits antimicrobial activity against human pathogens Yersinia enterocolitica, Shigella sonnei, S. flexneri and Streptococcus pneumoniae. Strain BGRA43 was able to survive in simulated gastric juice containing milk and retained cell number stability during the incubation in simulated intestinal conditions. In addition, LC/MS/MS analysis showed the ability of BGRA43 to hydrolyze β-lactoglobulin. Abundant growth of strain BGRA43 occurred in the presence of prebiotics inulin or concentrated oat bran β-glucan (Nutrim®), even when used as the sole carbon source. Similarly, strain BGRA43 grew satisfactorily in pure cow\u27s or goat\u27s milk as well as in the milk containing inulin or Nutrim®. Using the probiotic strain BGRA43 as a single starter strain, fermented milk products obtained from cow\u27s or goat\u27s milk with or without inulin or Nutrim® contained about 107 CFU/mL. The products were homogeneous and viscous and the best sensory scores were observed for fermented milk beverage made from reconstituted skimmed milk, whole cow\u27s milk and whole goat\u27s milk supplemented with 1 % inulin

Is Demonstrating the Concept of Multi-Use Too Soon for the North Sea?:Barriers and Opportunities from a Stakeholder Perspective

Multi-use (MU) has been promoted as a viable approach to the effective planning and mitigation of user-conflicts in the marine realm. Despite several research and pilot projects demonstrating the approach’s feasibility and benefits, commercially viable MU applications remain patchy and few. Further, MU is neither systematically applied nor purposively planned for even in the imminent event of incompatible and conflicting use of marine space. This paper seeks to identify barriers and opportunities for mainstreaming MU based on desktop study and iterative stakeholder consultation. The findings reveal that the MU concept was frequently framed as ‘co-location’ or ‘co-existence’ and aimed toward mitigating conflict among users. Practice was ahead of theory with little attention to synergistic and efficiency aspects. Barriers for MU application include shortcomings in legislation, sectoral thinking, and burdensome administrative procedures. The main opportunity lies in creating a conducive policy environment where MU risks and transaction costs become low and competitive, respectively. Solutions at the sea basin and national level, upon which further MU application can be anchored, are proposed

Noviji rezultati oplemenjivanja voćaka u Institutu za voćarstvo, Čačak

The Fruit Research Institute Čačak has seventy years long tradition in breeding of continental fruit species. The continual multi-disciplinary scientific and research work conducted so far has resulted in the releasing of 41 different cultivars. In addition to the named and released cultivars, for promising candidate cultivars have been selected (three plums and one peach) and entered for releasing, while there is also a large number of promising genotypes that are currently under intense evaluation. The paper presents an overview of the most significant biological and productive characteristic of the 12 new cultivars of pome and stone fruits, including seven plum cultivars (Boranka, Timočanka, Zlatka, Nada, Mildora, Krina and Pozna plava), two pear cultivars (Julijana and Anđelija) and three sour cherry cultivars (Sofija, Nevena and Iskra).Institut za voćarstvo, Čačak ima sedamdeset godina dugu tradiciju oplemenjivačkog rada na stvaranju novih sorti kontinentalnih vrsta voćaka. Do sada je kao rezultat kontinuiranog, multidisciplinarnog naučnoistraživačkog rada priznata 41 sorta različitih vrsta voćaka. Pored priznatih sorti, izdvojene su četiri kandidat sorte (tri šljive i jedna breskve) koje su u postupku priznavanja, kao i veliki broj perspektivnih genotipova koji se intenzivno proučavaju. U radu je prikazan pregled najznačajnijih bioloških i proizvodnih osobina 12 novije priznatih sorti jabučastih i koštičavih vrsta voćaka, i to: sedam sorti šljive (Boranka, Timočanka, Zlatka, Nada, Mildora, Krina i Pozna plava), dve sorte kruške (Julijana i Anđelija) i tri sorte višnje (Sofija, Nevena i Iskra)

Galectin-3 Deficiency Facilitates TNF-α-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection

Galectin-3 (Gal-3) has a role in multiple inflammatory pathways. Various, opposite roles of Gal-3 in liver diseases have been described but there are no data about the role of Gal-3 in development of hepatitis induced with cytomegalovirus infection. In this study we aimed to clarify the role of Gal-3 in murine cytomegalovirus (MCMV)-induced hepatitis by using Gal-3-deficient (Gal-3 KO) mice. Here we provide the evidence that Gal-3 has the protective role in MCMV-induced hepatitis. Enhanced hepatitis manifested by more inflammatory and necrotic foci and serum level of ALT, enhanced apoptosis and necroptosis of hepatocytes and enhanced viral replication were detected in MCMV-infected Gal-3 deficient mice. NK cells does not contribute to more severe liver damage in MCMV-infected Gal-3 KO mice. Enhanced expression of TNF-α in the hepatocytes of Gal-3 KO mice after MCMV infection, abrogated hepatocyte death, and attenuated inflammation in the livers of Gal-3 KO mice after TNF-α blockade suggest that TNF-α plays the role in enhanced disease in Gal-3 deficient animals. Treatment with recombinant Gal-3 reduces inflammation and especially necrosis of hepatocytes in the livers of MCMV-infected Gal-3 KO mice. Our data highlight the protective role of Gal-3 in MCMV-induced hepatitis by attenuation of TNF-α-mediated death of hepatocytes

Multi-use of the sea as a sustainable development instrument in five eu sea basins

This paper examines the concept of maritime multi-use as a territorial/SPATIAL governance instrument for the enhancement of sustainable development in five EU sea basins. Multi-use (MU) is expected to enhance the productivity of blue economy sectors, as well as deliver additional socio-economic benefits related to the environmental and social dimensions of sustainable development. The paper provides a definition of maritime multi-use and identifies the multi-uses with the highest potential in EU sea basins. In each sea basin, multi-use plays a different role as concerns sustainable development. For the Eastern Baltic Sea, the Mediterranean Sea and the Black Sea, the MU focus should remain on the environmental pillar of sustainable development. In the North Sea, North Atlantic and Western Baltic Sea, addressing social sustainability seems a key precondition for success of MU in enhancement of sustainable spatial development at sea. Moreover, it has been suggested to introduce MU key global strategies such as SDGs or Macroregional strategies and action plans and to supplement maritime spatial planning with sectoral incentives and educational efforts as key vehicles supporting MU. The paper concludes by identifying aspects which, in order to inform maritime spatial planning and maritime governance regarding a more conscious application of the aforementioned concept, require further investigation. Key tasks are related to: more profound evaluation of performance of policies supporting MUs, researching the impact of MU on societal goals and on the MU costs and benefits, including external ones, and finally identifying the impact of MU on the development of various sectors and regions on land

A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C

Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development