Analisis Pohon Berstruktur Menggunakan Metode CHAID pada Data Respons Ordinal

Eksplorasi data merupakan suatu hal yang penting dilakukan sebelum menganalisa data dengan metode lain. Salah satu metode eksplorasi untuk data respons kategorik adalah metode CHAID (Chi-square Automatic Interaction Detection). Tulisan ini berisi tentang kajian teori metode CHAID dan penerapannya yaitu menganalisis faktor-faktor yang berpengaruh pada peningkatan omset USAha anggota koperasi simpan pinjam (KSP) sebagai respons ordinal. Hasil kajian menunjukkan bahwa metode CHAID tidak hanya dapat digunakan sebagai metode eksplorasi tetapi juga mampu menggunakan struktur hubungan antara variabel respons kategorik dengan serangkaian variabel penjelas serta interaksi antar variabel penjelas. Struktur hubungan ini digambarkan dengan suatu pohon berstruktur

Differential depletion of total T cells and regulatory T cells and prolonged allotransplant survival in CD3Ɛ humanized mice treated with polyclonal anti human thymocyte globulin

<div><p>Thymoglobulin (ATG) is a polyclonal rabbit antibody against human thymocytes used as a T cell-depleting agent to prevent or treat allotransplant rejection. The aim of the present study was to investigate the effect of low dose ATG treatment exclusively on T cells using a humanized BALB/c human CD3Ɛ transgenic mouse model expressing both human and murine T cell receptors (TCR). Mice received a single intravenous (i.v.) injection of ATG. Blood and peripheral lymphoid organs were obtained after different time points. We found a significant T cell depletion in this mouse model. In addition, regulatory T cells (Tregs) proved to be less sensitive to depletion than the rest of T cells and the Treg:non-Treg ratio was therefore increased. Finally, we also investigated the effect of ATG in a heterotopic allogenic murine model of heart transplantation. Survival and transplant function were significantly prolonged in ATG-treated mice. In conclusion, we showed (a) an immunosuppressive effect of ATG in this humanized mouse model which is exclusively mediated by reactivity against human CD3Ɛ; (b) provided evidence for a relative resistance of Tregs against this regimen; and (c) demonstrated the immunomodulatory effect of ATG under these experimental circumstances by prolongation of heart allograft survival.</p></div

Murine cervical heart allotransplantation.

<p>BALB/c huCD3Ɛ were injected i.v with ATG or control rabbit Ig. Cervical heart transplantation was performed and graft function was evaluated by daily regular heart palpation. (A) Cardiac graft function and (B) graft survival (days). Graft function was expressed as the beating score, 0 no organ function, 1: fibrillation, only visible through magnification, 2: poor or partial organ function, 3: impairment in frequency or intensity of heart beating, 4: physiological organ function). Four to five mice per group, two independent experiments were performed. Data are shown as means ± SEM. Mann-Whitney statistical test was used, *p<0.05, ** p<0.001.</p