Metodología para evaluar efectos del deslumbramiento en amétropes corregidos

Light originated at a glare source and entering the eye, produces a veiling luminance that causes contrast decrease in retinal images and this effect could differ if the subject wears lenses or not. In this work the glare effects in subjects wearing lenses is studied inducing ammetropies in emmetropes. Contrast thresholds of sinusoidal patterns of 2 cpd and 0.5 cd/m2 are evaluated without and with glare, the glare source being steady, at 12o and producing 60 lx at the cornea. To exemplify the use of this methodology, 2 emmetropes under 8 different conditions are considered: naked eye; 3 control conditions (wearing neutral lenses) and 4 ammetropic conditions, inducing low (2 D) and medium (5 D) myopia and hyperopia with positive and negative contact lenses and correcting them with adequate ophthalmic lenses. The contrast threshold differences between the naked eye and the other 7 conditions are statistically not significant without glare and significant (up to more than 100%) with glare, independently of lens power and probably due to ectopic scattering and multiple reflections. The method proposed enables the determination of the glare effects if lenses are worn though a greater population is required to attain conclusive data.La luz originada en una fuente deslumbrante que entra al ojo, produce una luminancia de velo que causa una disminución de contraste en las imágenes retinianas y este efecto podría diferir si el sujeto usa lentes o no. En este trabajo se estudian los efectos del deslumbramiento en sujetos que usan lentes induciendo ametropías en emétropes. Se evalúan contrastes umbrales de patrones sinusoidales de 2 cpd y 0.5 cd/m2 sin y con deslumbramiento, la fuente deslumbrante siendo estable, a 12º y produciendo 60 lx en la córnea. Para ejemplificar el uso de esta metodología, se consideran 2 emétropes en 8 condiciones diferentes: ojo desnudo; 3 condiciones de control (lentes neutras) y 4 condiciones ametrópicas, induciendo miopía e hipermetropía baja (±2 D) y media (±5 D) con lentes de contacto positivas y negativas y corrigiéndolas con adecuadas lentes oftálmicas. Las diferencias de contraste entre el ojo desnudo y las otras 7 condiciones son estadísticamente no significativas sin deslumbramiento y significativas (hasta más de 100%) con deslumbramiento, independientemente de la potencia de la lente y probablemente debido a scattering ectópico y reflexiones múltiples. El método propuesto permite determinar los efectos de deslumbramiento al usar lentes aunque, para tener datos contundentes, se requiere una mayor poblaciónFil: de Paul Camacho, Anibal Gabriel. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Luminotecnia, Luz y Vision; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; ArgentinaFil: Colombo, Elisa Margarita. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Luminotecnia, Luz y Vision; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; ArgentinaFil: Comastri, Silvia Ana Elva. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Fisica; ArgentinaFil: Aparicio, Juan A.. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Menéndez, José Antonio. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Issolio, Luis Alberto. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Luminotecnia, Luz y Vision; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; Argentin

Implementation of the frequency scatter index in clinical commercially available double-pass systems

A previous work has reported a methodology to quantify intraocular scattering using a high sensitivity double-pass instrument with a robust index, the frequency scatter index. The purpose of our study was to evaluate an adaptation of the frequency scatter index for use in clinical double-pass systems. A prospective observational study was carried out in a group of patients with nuclear cataracts (n = 52) and in a control group (n = 11) using conventional double-pass systems. The frequency scatter index and the objective scatter index were used to assess the scattering. The Spearman coefficient was calculated to assess the correlation between both indexes, obtained from the double-pass images. Simultaneous measurements were performed with a double-pass and with a Hartmann-Shack wavefront sensor in the control group. The root-mean-square wavefront error and the full width at half maximum of the double-pass image were used to quantify the residual aberrations introduced by the variations in pupil size and retinal eccentricity. Measurement in eyes with different grades of cataracts shows a strong correlation (¿ = 0.929, p .05). We have introduced and evaluated an adaptation of a methodology proposed recently for the measurement of intraocular scattering using the double-pass technique with a robust index, which is less affected by ocular aberrations. The frequency scatter index can be applied to conventional double-pass instruments available in clinical environments.Peer ReviewedPostprint (updated version

Photoreceptor contributions to the human pupil light reflex

The pupil reacts to the amount of light reaching the eye. It reduces its size when the light is high and dilates at low light levels to allow the entrance of more photons. This behavior is called pupil light reflex (PLR). Recent investigations in humans were conducted to understand how the photoreceptor signals are combined to drive the pupil light reflex. This review is about the physiological processes that govern the pupil in humans. In particular, how cone-opsins, rhodopsin, and melanopsin photoreception contribute to governing the PLR. We also summarize investigations on the assessment of the PLR in clinical settings

Light adaptation in letter contrast sensitivity: The influence of optical and neural mechanisms

This work discusses the level of implication of the optical and physiological mechanisms in letter contrast sensitivity (CS) under different conditions of environmental lighting. A study was carried out on 26 eyes with normal ocular health. Sixteen conditions of light, obtained by combining different test luminances (from 10 to 600 cd/m2) and surround luminances (from 1 to 600 cd/m2) were considered. The results reveal a significant influence of optical factors (pupil size variations and glare effects) on CS when surround luminance changes, and dominance of neural effects when test luminance changes. Furthermore, test size and illumination conditions are provided for which letter threshold contrasts are not sensitive to surround luminance changes.Fil: Arranz, I.. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Matezanz, B. M.. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Issolio, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucuman. Instituto de Investigacion En Luz, Ambiente y Vision; Argentina. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnologia. Departamento de Luminotecnia, Luz y Vision; ArgentinaFil: Menéndez, J. A.. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Mar, S.. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Aparicio, J. A.. Universidad de Valladolid. Facultad de Ciencias; Españ

Temporal retinal sensitivity in mesopic adaptation

Purpose: Night driving is a complex visual task with important ramifications for driver and pedestrian safety. It is usually performed under mesopic or scotopic conditions and frequently, in the presence of transient glare sources that can adapt parts of the central retina. The objective of this work was to analyze the time response of adaptation for the central 15° of the retina when part of it is exposed to transient or steady mesopic adapting fields. Methods: Absolute visual thresholds and luminance thresholds when viewing steady and transient adaptation fields were measured for three observers, at temporal retinal eccentricities of 0°-14.5° in steps of 2.9° (subsequently described as 0°, 3°, 6°, 9°, 12° and 15°) using a two-channel Maxwellian view optical system. The adaptation field and stimulus subtended 1.05° and 0.45° respectively. The transient adaptation field was presented with a stimulus onset asymmetry (SOA) of 300ms. Time course adaptation curves were also measured at 0°, 6° and 9° Results: The absolute dark adaptation threshold (threshold measured at dark adaptation conditions or ) decreases in peripheral retina due to an increasing rod contribution. Luminance thresholds vs eccentricity curves for transient () and steady () mesopic adaptation fields intersect across the first 15° of the peripheral retina. Conclusions: While the fovea shows higher sensitivity than the areas of peripheral retina investigated in this study, the speed of adaptation, measured from the visibility loss, is greater for retinal regions between 6° and 9° than for the fovea or retinal eccentricities beyond 9°.Fil: Beatriz Matezanz. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Issolio, Luis Alberto. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Laboratorio de Luminotecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Investigación en Luz, Ambiente y Visión. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Instituto de Investigación en Luz, Ambiente y Visión; ArgentinaFil: Isabel Arranz. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Cristina de la Rosa. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: José Menéndez. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Santiago Mar. Universidad de Valladolid. Facultad de Ciencias; EspañaFil: Juan Antonio Aparicio. Universidad de Valladolid. Facultad de Ciencias; Españ

Transmittance measurement of the in vivo human eye with a double-pass system

Purpose: To develop a methodology based on a double-pass system to obtain information about the transmittance of ocular media. Methods: The procedure consists of recording double-pass images at different powers of a laser diode of 780 nm and determining the scattering in an area between 25–35 arcmin of each image. The scattering showed linear behavior in respect to the irradiance of the laser, and the slope of the linear fit was proportional to the transmittance squared of the media evaluated. An artificial eye with different filters was tested first. Then, fifteen subjects with clear ocular media were divided into two groups: ten subjects classified by the iris color were recruited for the measurements of an ocular transmittance index and the estimation of the transmittance (group A), and another five subjects were selected for measurements with neutral filters (group B). Results: The measurements performed in group A presented a mean transmittance of 42.95%. No differences in the transmittance were found between subjects with different iris color ( p = 0.154). Measurements in group B showed a good correlation (r = 0.959, p < 0.001) between the expected and the measured value for the transmittance. Conclusion: We proposed and evaluated a method to determine the transmittance of the eye in vivo using the double-pass system.Peer ReviewedPostprint (published version

Influence of background size, luminance and eccentricity on different adaptation mechanisms

Mechanisms of light adaptation have been traditionally explained with reference to psychophysical experimentation. However, the neural substrata involved in those mechanisms remain to be elucidated. Our study analyzed links between psychophysical measurements and retinal physiological evidence with consideration for the phenomena of rod-cone interactions, photon noise, and spatial summation. Threshold test luminances were obtained with steady background fields at mesopic and photopic light levels (i.e., 0.06-110 cd/m2) for retinal eccentricities from 0° to 15° using three combinations of background/test field sizes (i.e., 10°/2°, 10°/0.45°, and 1°/0.45°). A two-channel Maxwellian view optical system was employed to eliminate pupil effects on the measured thresholds. A model based on visual mechanisms that were described in the literature was optimized to fit the measured luminance thresholds in all experimental conditions. Our results can be described by a combination of visual mechanisms. We determined how spatial summation changed with eccentricity and how subtractive adaptation changed with eccentricity and background field size. According to our model, photon noise plays a significant role to explain contrast detection thresholds measured with the 1/0.45° background/test size combination at mesopic luminances and at off-axis eccentricities. In these conditions, our data reflect the presence of rod-cone interaction for eccentricities between 6° and 9° and luminances between 0.6 and 5 cd/m2. In spite of the increasing noise effects with eccentricity, results also show that the visual system tends to maintain a constant signal-to-noise ratio in the off-axis detection task over the whole mesopic range.Fil: Gloriani, Alejandro H.. Universidad de Valladolid; EspañaFil: Matesanz, Beatriz M.. Universidad de Valladolid; EspañaFil: Barrionuevo, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Investigación en Luz, Ambiente y Visión. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Instituto de Investigación en Luz, Ambiente y Visión; ArgentinaFil: Arranz, Isabel. Universidad de Valladolid; EspañaFil: Issolio, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Investigación en Luz, Ambiente y Visión. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Instituto de Investigación en Luz, Ambiente y Visión; ArgentinaFil: Mar, Santiago. Universidad de Valladolid; EspañaFil: Aparicio, Juan A.. Universidad de Valladolid; Españ

Quantitative and functional influence of surround luminance on the letter contrast sensitivity function

Aim: To determine the influence of surround luminance on the letter contrast sensitivity function. Method: The binocular contrast sensitivities of 31 young and ocularly healthy individuals were measured with letters of sizes calculated to obtain the targeted fundamental frequencies of 3, 10, 20, and 30 c deg-1, respectively; with surround luminances from 1 to 1000 cd m-2, always with a test chart background luminance of 200 cd m -2. Results: The letter contrast sensitivity increased with surround luminance up to 100 cd m-2 and decreased when surround luminance increased from 100 to 1000 cd m-2. These increments are larger for higher fundamental spatial frequencies, while decrements are similar for all frequencies. To analyse pupil size influence, results were compared with theoretical predictions obtained by combining different ocular MTFs with a typical neural function, where pupil size decrease leads to letter contrast sensitivity increments and veiling luminance causes the observed decrements. Other possible optical or neural factors that influence these values have also been considered. Conclusions: Letter contrast sensitivity function depends on surround luminance and this influence should be considered in future standardized directives.Fil: Juan Antonio Aparicio. No especifica;Fil: Isabel Arranz. No especifica;Fil: Beatriz Matezanz. No especifica;Fil: Juan Vizmanos. No especifica;Fil: L Padierna. No especifica;Fil: V. Gonzáles. No especifica;Fil: Juan Menéndez. No especifica;Fil: Santiago Mar. No especifica;Fil: Issolio, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Investigación en Luz, Ambiente y Visión. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Instituto de Investigación en Luz, Ambiente y Visión; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Luminotecnia, Luz y Visión; Argentin