15 research outputs found

    BEEING FREE: HABBITS AND DAILY BREAKOUTS IN PRISON ENVIRONMENT

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    Prison is the last possible destination for a humanistic society where the community exert its social, moral and normative borders. A total institution that offers a low mobility and a diminished level of freedom may have contrary effects towards society expectations. Neutralizing the individual for limited periods cannot be sufficient in a society of communication and openness towards others. This utopian society may be considered a social mirror that reflects the failure of the community to accommodate and operate human adaptability. The concepts of freedom, family, visit and imprisonment routine are transposed to a qualitative analyze after interviewing 37 inmates from Bucharest-Jilava Penitentiar

    Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes

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    The electrocatalytical property of single-wall carbon nanotube (SWNT)modified electrode toward NADH detection was explored by cyclic voltammetry andamperometry techniques. The experimental results show that SWNT decrease theovervoltage required for oxidation of NADH (to 300 mV vs. Ag/AgCl) and this propertymake them suitable for dehydrogenases based biosensors. The behavior of the SWNTmodified biosensor for L-malic acid was studied as an example for dehydrogenasesbiosensor. The amperometric measurements indicate that malate dehydrogenase (MDH)can be strongly adsorbed on the surface of the SWNT-modified electrode to form anapproximate monolayer film. Enzyme immobilization in Nafion membrane can increasethe biosensor stability. A linear calibration curve was obtained for L-malic acidconcentrations between 0.2 and 1mM

    A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages

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    Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol–gel matrix. Working parameters such as applied potential, pH, NAD+ concentration, storage conditions were optimized. A response range between 0.05 and 2 mM was found with a sensitivity of 13.45 ± 0.67 µA/mM·cm2 and a detection limit of 20 µM. The developed biosensor was used to detect ethanol in commercial beverages with good accuracy

    A New Highly Sensitive Electrochemical Biosensor for Ethanol Detection Based on Gold Nanoparticles/Reduced Graphene Oxide/Polyallylamine Hydrochloride Nanocomposite

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    A highly sensitive electrochemical biosensor for ethanol based on a screen-printed electrode modified with gold nanoparticles—electrochemically reduced graphene oxide—poly (allylamine hydrochloride) nanocomposite (AuNPs-ERGO-PAH) is reported in this work. Ethanol was oxidized in the presence of the oxidized form of the nicotinamide adenine dinucleotide (NAD+) in a reaction catalyzed by alcohol dehydrogenase (ADH) immobilized in sol-gel. The AuNPs-ERGO-PAH nanocomposite was used as a transducer for the electrocatalytic oxidation of the reduced form the nicotinamide adenine dinucleotide (NADH) produced in the enzyme reaction. Under the optimal conditions, the ethanol biosensor exhibits a wide dynamic range from 0.05 to 5 mM with a low detection limit of 10 µM (S/N = 3) and a high sensitivity of 44.6 ± 0.07 µA/mM·cm2 for the linear range between 0.05 and 0.2 mM. The biosensor response was stable for up to 6 weeks. Furthermore, the developed biosensor has been used to detect ethanol in alcoholic beverages with good results, suggesting its potential application in various fields, including fermentation processes and food quality control

    Amperometric L-Lactate Biosensor Based upon a Gold Nanoparticles/Reduced Graphene Oxide/Polyallylamine Hydrochloride Modified Screen-Printed Graphite Electrode

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    This work describes a novel L-lactate biosensor based on the immobilization of L-lactate dehydrogenase enzyme on the screen-printed electrode modified with a ternary composite based on gold nanoparticles, electrochemically-reduced graphene oxide, and poly (allylamine hydrochloride). The enzyme was stabilized by crosslinking with glutaraldehyde. Applied working potential, pH and NAD+ concentration were optimized. The biosensor reports a specific sensitivity of 1.08 µA/mM·cm2 in a range up to 3 mM L-lactic acid with a detection limit of 1 µM. The operational and long-term stability as well as good selectivity allowed the L-lactic acid measurement in dairy products and wine samples

    Enhanced Sensitive Love Wave Surface Acoustic Wave Sensor Designed for Immunoassay Formats

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    We report a Love wave surface acoustic wave (LW-SAW) immunosensor designed for the detection of high molecular weight targets in liquid samples, amenable also for low molecular targets in surface competition assays. We implemented a label-free interaction protocol similar to other surface plasmon resonance bioassays having the advantage of requiring reduced time analysis. The fabricated LW-SAW sensor supports the detection of the target in the nanomolar range, and can be ultimately incorporated in portable devices, suitable for point-of-care testing (POCT) applications

    A New Integrated System for Assistance in Communicating with and Telemonitoring Severely Disabled Patients

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    In this paper, we present a new complex electronic system for facilitating communication with severely disabled patients and telemonitoring their physiological parameters. The proposed assistive system includes three subsystems (Patient, Server, and Caretaker) connected to each other via the Internet. The two-way communication function is based on keywords technology using a WEB application implemented at the server level, and the application is accessed remotely from the patient’s laptop/tablet PC. The patient’s needs can be detected by using different switch-type sensors that are adapted to the patient’s physical condition or by using eye-tracking interfaces. The telemonitoring function is based on a wearable wireless sensor network, organized around the Internet of Things concept, and the sensors acquire different physiological parameters of the patients according to their needs. The mobile Caretaker device is represented by a Smartphone, which uses an Android application for communicating with patients and performing real-time monitoring of their physiological parameters. The prototype of the proposed assistive system was tested in “Dr. C.I. Parhon” Clinical Hospital of IaÅŸi, Romania, on hospitalized patients from the Clinic of Geriatrics and Gerontology. The system contributes to an increase in the level of care and treatment for disabled patients, and this ultimately lowers costs in the healthcare system
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