7 research outputs found
Вплив поліморфізму генів PTHR1 та COL1A1 на розвиток захворювань тканин пародонта у людей молодого віку
At the present stage of development of dentistry, the leading Ukrainian and foreign scientists devote a considerable part of the research to a deeper study of the etiology and pathogenetic mechanisms of periodontal tissue diseases and the influence of various exogenous and endogenous factors on their course.The aim of the study – to learn the ability and methods of forecasting and early diagnosis of the periodontal tissue lesions in young people. Materials and Methods. During our research we examined 24 young people with periodontal tissue diseases, who were included to the main group, and 15 healthy people, who formed the control group. The complex clinical examination was performed in each research group. It was studied the distribution of polymorphous variants of the type I parathormone receptor and the α1-chain of collagen gene with a help of polymerase chain reaction by restrictase cleavage of DNA fragments and electrophoresis in polyacrylamide gel (AA/BA 29:1). Results and Discussion. The distribution of genotypes by PTHR1 gene in control group was similar to those in main group (p>0.05). Also we didn’t find the difference between frequencies of the separate alleles in people with periodontal tissue pathology and without it (p>0.05). Yes, the repetitions of the allele 5 encoding normal type I parathormone receptor were found more often, comparing with the allele 6 that is responsible for the formation of unfunctional PTHR1 (р<0.001) in both main and control groups. The dominance of the genotype TT, which corresponds to the pathology, was found in young people with the periodontal tissue lesions – (38.46 ± 4.79) %, while among the control group the genotype of norm GG was met the most often – (68.24±5.08) %. Also, the frequency of repetitions of the allele T encoding the imperfect collagen chain was (57.60±3.79) % in young people with periodontal diseases, and in the control group this figure was (13.27±2.81) %, p<0.001. Conclusions. According to our results the presence of allele T and genotype TT that correspond the imperfect collagen chain may be one of the causes of periodontal tissue pathology.На современном этапе развития стоматологии значительную часть исследований ведущие украинские и зарубежные ученые отрасли посвящают более глубокому изучению этиологии и патогенетических механизмов заболеваний тканей пародонта и влияния различных экзогенных и эндогенных факторов на их ход.Цель исследования – изучить возможности и методы прогнозирования и ранней диагностики поражений тканей пародонта у людей молодого возраста.Материалы и методы. В процессе работы било обследовано 24 молодых людей с заболеваниями тканей пародонта, что составили основную группу и 15 здоровых лиц, что вошли в контрольную группу. В каждой исследуемой группе было проведено полное клиническое обследование. С помощью полимеразной цепной реакции путем расщепления фрагментов ДНК рестриктазами и электрофореза в 8 % полиакриламидном геле (сток-раствор АА/БА 29:1) било изучено распределение полиморфных вариантов гена рецептора паратгормона 1 типа и α1-цепи коллагена.Результаты исследования и их обсуждение. В группе контроля распределение вариантов генотипа за геном PTHR1 почти не отличалось (p>0,05) от такого в основной группе. Так же не было обнаружено разницы между частотами определения отдельных аллелей в лиц с патологией тканей пародонта и без нее (p>0,05). Да, повтори аллеля 5, что кодирует нормальный рецептор паратгормона 1 типа, были гораздо чаще, в сравнении с аллелем 6, что ответственный за формирование неполноценного PTHR1 (р<0,001) как в основной так и в контрольной группах. В молодых людей из поражённым пародонтом было обнаружено преобладание генотипа TT, что соответствует патологии – (38,46±4,79) %, а в контрольной группе наиболее часто встречался генотип нормы GG (68,24±5,08) %. Также частота повторов аллеля T, что кодирует дефектную цепь коллагена, составляла (57,60±3,79) % в молодых людей с заболеваниями тканей пародонта, а в группе контроля этот показатель соответствовал (13,27±2,81) %, р<0,001.Выводи. Согласно данным, полученным в процессе исследованя, наличие аллеля T и генотипа TT, что соответствует дефектной цепи коллагена, определяет склонность и может бить одной из причин развития патологии тканей пародонта.На сучасному етапі розвитку стоматології значну частину досліджень провідні українські та зарубіжні науковці галузі присвячують більш глибокому вивченню етіології та патогенетичних механізмів захворювань тканин пародонта та впливу різних екзогенних та ендогенних факторів на їх перебіг.Мета дослідження – вивчити можливості та способи прогнозування і ранньої діагностики уражень тканин пародонта у людей молодого віку.Матеріали і методи. В процесі роботи було обстежено 24 молодих людей із захворюваннями тканин пародонта, що склали основну групу, та 15 здорових осіб, які ввійшли до контрольної групи. У кожній досліджуваній групі було проведено повне клінічне обстеження. За допомогою полімеразної ланцюгової реакції шляхом розщеплення фрагментів ДНК рестриктазами та електрофорезу в 8 % поліакриламідному гелі (сток-розчин АА/БА 29:1) було вивчено розподіл поліморфних варіантів гена рецептора паратгормону 1 типу та α1-ланцюга колагену.Результати досліджень та їх обговорення. В групі контролю розподіл варіантів генотипу за геном PTHR1 майже не відрізнявся (p>0,05) від такого в основній групі. Так само не було виявлено значущої різниці між частотами виявлення окремих алелей в осіб із патологією тканин пародонта та без неї (p>0,05). Так, повтори алеля 5, що кодує нормальний рецептор паратгормону 1 типу, були значно частіше, порівняно з алелем 6, що відповідає за формування неповноцінного PTHR1 (р<0,001) як в основній, так і у контрольній групах. У молодих людей із ураженим пародонтом було виявлено переважання генотипу TT, що відповідає патології (38,46±4,79) %, а в контрольній групі найчастіше зустрічався генотип норми GG (68,24±5,08) %. Також частота повторів алеля T, що кодує недосконалий ланцюг колагену, становила (57,60±3,79) % в молодих людей із захворюваннями тканин пародонта, а в групі контролю цей показник складав (13,27±2,81) %, р<0,001.Висновки. Згідно з даними, отриманими в процесі дослідження, наявність алеля T та генотипу TT, що відповідають недосконалому ланцюгу колагену, визначає схильність та може бути однією із причин розвитку патології тканин пародонта
Особливості перебігу карієсу зубів у дітей із захворюваннями шлунково-кишкового тракту
The chronic diseases of the gastrointestinal tract and frequent exasperation may lead to decreased metabolism and immunologic reactivity that assists to development of tooth decay. The research aim was to study the indexes of prevalence and intensity of tooth decay for children with the diseases of gastrointestinal tract. For the decision of the goаl of the work were inspected 357 children 6‒15 years–old. As a result of undertaken studies there was found that high prevalence (84.31±1.93.%) and intensity (8.96±0.15 tooth) of caries was determined in children with the gastrointestinal tract diseases, with the expressed tendency to complications, with predominating of the subcompensated and decompensated forms of caries that made progress with the increase of age of this group of patients.Длительное течение хронических заболеваний пищеварительной системы и частые обострения приводят к нарушению всех видов обмена, снижения иммунологической реактивности, которая способствует развитию кариеса зубов. Целью исследования было изучение показателей распространенности и интенсивности кариеса зубов у детей с заболеваниями желудочно-кишечного тракта. Для решения цели работы были обследованы 357 детей 6–5-летнего возраста. В результате проведенных исследований установлено, что у детей с заболеваниями ЖКТ определялась высокая распространенность (84,31±1,93) % и интенсивность (8,96±0,15) зуба кариозного процесса с выраженной тенденцией к осложнениям, с превалированием субкомпенсированных и декомпенсированных форм данной болезни, которая прогрессировала с увеличением возраста данной группы пациентов.Тривалий перебіг хронічних захворювань травної системи та часті загострення призводять до порушення усіх видів обміну, зниження імунологічної реактивності, що сприяє розвитку карієсу зубів. Метою дослідження було вивчення показників поширеності та інтенсивності карієсу зубів у дітей із захворюваннями шлунково-кишкового тракту. Для вирішення мети роботи було обстежено 357 дітей 6‒15-річного віку. В результаті проведених досліджень встановлено, що у дітей із захворюваннями ШКТ визначалась висока поширеність (84,31±1,93) % та інтенсивність (8,96±0,15) зуба каріозного процесу з вираженою тенденцією до ускладнень, із превалюванням субкомпенсованих та декомпенсованих форм даної хвороби, що прогресувала зі збільшенням віку даного контингенту пацієнтів
The evaluation of risk factors of genesis of plural caries in children with pathology of gastrointestinal tract
The substantial risk factors of development of plural caries in children with gastrointestinal diseases are toxicosis of pregnancy, extragenital mother diseases, children diseases of junior age,inferior feed, irregular oral hygiene. The research aim was to study risk factors of genesis of plural caries in children with gastrointestinal diseases.For thedecision of aim were inspected260 children 6‒15-years-old.The result of undertaken studies: a relative risk ofdevelopment of caries
at pathology of pregnancyand luingins in children with a plural carieswas in 1,4 times higher thanfor childrenwithout pathology.In the same time, functional disorders of GIT,oligotrophyand rachitis, children diseases of junior age ensure development of thedecompensated form of caries,with a relative
risk,in 2,25 timeshigher than for childrenwithout mentionedpathologies
THE MODERN ASPECTS OF THE RAMPANT CARIES AND THE INFLUENCE OF GASTROINTESTINAL TRAC DISEASES ON THE ORGANISMS OF THE ORAL CAVITY IN CHILDREN (REVIEW OF THE LITERATURE)
An important role in pathogenesis of tooth decay is played by the state of digestion system. The chronic diseases of gastrointestinal tract (stomach, liver, duodenum, pancreas) are accompanied by the deficiency of vitamins, mineral substances, proteins and carbohydrates in organism that results in development of inflammatory and dystrophic changes in oral cavity tissues. The chronic diseases of the gastrointestinal tract and frequent exasperation may lead to decreased metabolism and immunologic reactivity that assists to development of tooth decay. The substantial risk factors of development of plural caries in children with gastrointestinal diseases are toxicosis of pregnancy, extragenital mother diseases, children diseases of junior age, inferior feed, irregular oral hygiene. Today significant achievements have been made in the study of the etiology and pathogenesis of caries of the teeth. The commonly recognized mechanism of caries is the progressive demineralization of solid tissues of the tooth under the influence of organic acids, the formation of which is associated with the activity of certain microorganisms. The course of rampant caries in children on the background of general-somatic diseases is characterized by rapid, malignant course and most often leads to complications, in particular, to the emergence of secondary and relapsing caries. Such patients are more likely to experience loss of and violation of the boundary adherence of filling, which dictates the need for measures aimed at improving the treatment and prevention of caries, especially its decompensated form. In patients with decompensated form of caries, changes in the parameters of local humoral immunity are revealed. As a rule, the saliva of such patients is viscous, the rate of secretion is low. The increased content of ionized and total calcium, phosphates, mineral salting potential of saliva decreases, and the total concentration and amount of active ions of sodium and potassium is increased. At multiple caries, increased activity of amylase and phosphatase in the oral fluid
Mechanism of changes of peripheral neuromuscular endings of the tongues of rats with experimental streptozotocyn diabetes mellitus
This paper presents the characteristics of dynamics of morphological changes of neuromuscular endings of the tongues of rats with experimentally induced streptozotocin diabetes mellitus. The hysto-ultrastructural research showed pathomorphological changes in different periods of the experimental diabetes mellitus. The patterns of these changes indicate the close interrelation between neuromuscular endings and elements of muscular tissues. During experimental streptozotocin diabetes mellitus, there occured interconnected structural changes of muscle fibers, myelin nerve fibers and the microcirculatory channel of the tongue with damage to the neuromuscular endings. In the dynamics of the diabetic process, three phases were distinguished: the phase of reactive-dystrophic changes with maximum severity two weeks after the beginning of the experimental modeling of streptozotocin diabetes mellitus; the phase of destructive-dystrophic processes, which began after the fourth week from the beginning of the modeling of experimental streptozotocin diabetes; the degenerative-destructive phase, which developed after the sixth week of the experiment. The dynamics of changes in the neuromuscular endings are also related to the duration of diabetes mellitus, they occur in two stages: in the first stage (up to 4 weeks) reactive processes were observed, in the second (6–8 weeks) – dystrophic processes. The uneven degree of their manifestation is related to the reaction of the microcirculatory channel of neuromuscular endings and composition of the tongue muscles. The greatest sensitivity to hyperglycemia was observed in muscle fibers of the intermediate type
HISTOLOGICAL CHANGES OF THE KIDNEYS, LIVER AND LUNGS WHICH APPEARS IN LABORATORY ANIMALS AFTER MODELING OF CRANIO-SKELETAL TRAUMA AND ITS CORRECTION BY PHYTOMIXTURE
In the late period after cranio-skeletal trauma, the stimulation of reparative processes in organs and systems of the body is very important. In these circumstances, the effectiveness of phyto-medicines was not insufficiently studied. Phytho-pharmaceuticals include a number of natural biologically active substances capable of overcoming the mechanisms of damage and stimulating reparative processes. The absence of side effects and the background of high biological activity makes them a promising tool for correction of various pathological disorders and processes. The purpose of this work is to find out the histological changes of the kidneys, liver and lungs which appears in laboratory animals after modeling of cranio-skeletal trauma and correction of these changes by phytomixture. In experiments was used 99 non-linear white rats, 180-200 grams weight, which were on a standard vivarium diet. All animals were divided into three groups: control and two experimental. In both experimental groups (46 animals), under the conditions of thiopental-sodium anesthesia (40 mg per 1 kg body weight intraperitoneally), a moderate severity of closed craniocerebral trauma was modeled and caused a closed fracture of the femoral bones. The control group included 7 intact animals. After 14 days of post-traumatic period, for the first experimental group, the phytochemicals, which included Arnica montana, Elytrigia repens, Calendula officinalis, Urtica dioica and folium Betulae were used. The infusion was prepared at 1:10 and administered once a day to the experimental animals through the probe into the stomach at a dose of 10 ml per kilogram of animal mass daily from day 14 to day 35 of the experiment. In the second experimental group, an equivalent volume of physiological solution was intra gastrically injected during observation periods. The surviving animals, in conditions of thiopental-sodium anesthesia (80 mg per 1 kg of body weight intraperitoneally), after 14, 21, 28 and 35 days after trauma were taken out from the experiment by the method of total blood-flow from the heart. The erythrocyte index of intoxication (EII) and the content of fractions of medium weight molecules at 254 and 280 nm (MSM254, MSM280) were determined. In terms of cranio-skeletal injuries modeling during the late traumatic disease manifestations a significant increase of the level of endogenous intoxication was observed. It was manifested by increase of erythrocyte index value intoxication (EI) and content fractions average molecular weight (MSM) in the blood serum. The level of EI was significantly greater than control in all periods of observation with a moderate decline after 35 days. The characteristic feature of the dynamics of content MSM serum fractions is their high level after 14 days with normalization after 28 days and re-growth after 35 days. After applying phytomixture a significant decrease in endotoxemia was observed. Value of EI reaches the level of control after 35 days, and fractions MSM - starting from 28 days. Thus, after 21 days of the experiment in the stromal components of the kidneys, liver and lungs uneven blood supply mainly the venous channel was visualized. It was combined with moderate swelling of interstitium and the formation of focal polymorphic-cell infiltrates. In epitheliocytes there was mainly protein degeneration. The use of the herbal extract reduced perivascular edema and polymorphocyte infiltration of interalveolar lobes in the lungs, the development of the degeneration of the epithelium of the excretory renal tubule and hepatocytes. The appearance of dual-core cells showed an increase in regenerative activity in the liver and in the kidneys. There were discovered that simulated trauma is accompanied by dystrophic-necrotic disorders of the liver, kidney and lung tissues, which decrease until 35 days of the experiment, with the appearance of marked signs of regeneration. After the phytomixture using the signs of tissue recovery were more pronounced, especially after 35 days after injury
Indices of the dental caries intensity in individuals with different clinical variations of psychophysiological disadaptation
The role of psychological characteristics of the personality in the emergence and development of dental diseases has been substantiated by a number of studies of domestic and foreign scientists, in which features of the emotional and personal sphere of patients with different resistance of hard dental tissues and periodontium to diseases were considered.
The purpose of the study was to study the indices of caries intensity in people with periodontal disease in various clinical variants of psychophysiological disadaptation.
Materials and methods. Determination of the intensity of dental caries (DMF index) was carried out among 90 young people (aged18-44 years) with periodontal diseases and considering their psychophysiological conditions.
Results. While analyzing the values of the DMF index in patients in the experimental groups, it was eatablished that in group I, the mean DMF value (5.42 ± 0.18) indicated a low intensity of the carious process, which was 1.3 times lower than the mean values of the DMF (7.10 ± 0,19) in individuals of group II, whose values of DMF indicated a moderate intensity of caries