Pyroxene low-temperature plasticity and fragmentation as a record of seismic stress evolution in the lower crust

Seismic rupture of the lower continental crust requires a high failure stress, given large lithostatic stresses and potentially strong rheologies. Several mechanisms have been proposed to generate high stresses at depth, including local amplification of stress heterogeneities driven by the geometry and rheological contrast within a shear zone network. High dynamic stresses are additionally associated with the subsequent slip event, driven by propagation of the rupture tips. In the brittle upper crust, fracturing of the damage zone is the typical response to high stress, but in the lower crust, the evolution of combined crystal plastic and brittle deformation may be used to constrain in more detail the stress history of rupture, as well as additonal parameters of the deformation environment. It is crucial to understand these deep crustal seismic deformation mechanisms both along the fault and in the wall rock, as coseismic damage is an important (and sometimes the only) method of significantly weakening anhydrous and metastable lower crust, whether by grain size reduction or by fluid redistribution.A detailed study of pyroxene microstructures are used here to characterise the short-term evolution of high stress deformation experienced on the initiation of lower crustal earthquake rupture. These pyroxenes are sampled from the pseudotachylyte-bearing fault planes and damage zones of lower crustal earthquakes linked to local stress amplifications within a viscous shear zone network, recorded in an exhumed granulite-facies section in Lofoten, northern Norway. In orthopyroxene, initial low-temperature plasticity is overtaken by pulverisation-style fragmentation, generating potential pathways for hydration and reaction. In clinopyroxene, low-temperature plasticity remains dominant throughout but the microstructural style changes rapidly through the pre- and co-seismic periods from twinning to undulose extinction and finally the formation of low angle boundaries. We present here an important record of lower crustal short-term stress evolution along seismogenic faults

Strain localization in pseudotachylyte veins at lower crustal conditions

Viscous shearing in the dry and strong lower crust often localizes in pseudotachylyte veins (i.e. quenched molten rocks formed by the frictional heat released during seismic slip), and it has been suggested that brittle (coseismic) grain-size reduction and fluid infiltration in the fractured domains are necessary to weaken the anhydrous granulitic lower crust. However, the deformation mechanisms responsible for the associated strain weakening and viscous shear localization in pseudotachylytes are yet to be explored. This study investigates the deformation microstructures of mylonitized pseudotachylytes in anorthosites from Nus- fjord, northern Norway, where ductile shear zones invariably nucleate in pseudotachylyte veins. Thus, pseudotachy- lytes are weaker than the host rock during superposed ductile deformation. Pristine pseudotachylytes contain microlites of plagioclase, clinopyroxene, amphibole and orthopyroxene, flow structures, and chilled margins. Some pseudotachylytes have lost the pristine microstructure and have recrystallized into a fine-grained ( < 10 \u3bc m) mixture of plagioclase, amphibole, clinopyroxene, biotite, quartz \ub1 K-feldspar \ub1 orthopyroxene. Thus, the fine grain size in the mylonites ( < 20 \u3bc m) is not the product of progressive grain-size reduction with increasing strain, but is an initial characteristic of the shear zone (pseudotachylyte) precursor. The stable mineral assemblage in the mylonitic foliation consists of plagioclase, hornblende, clinopyroxene \ub1 quartz \ub1 biotite \ub1 orthoclase. Geothermobarometry and thermodynamic modelling indicate that pristine pseudotachylytes and their mylonitized equivalents formed at ca. 700 \u30aC and 0.6-0.9 GPa. Diffusion creep and grain boundary sliding were identified as the main deformation mechanisms in the mylonite on the basis of the lack of crystallographic preferred orientations, the high degree of phase mixing, and the nucleation of hornblende in dilatant sites. In contrast with common observations that fluid infiltration is required to trigger viscous deformation, thermody- namic modelling indicates that a limited amount of fluid (0.4 wt%, similar to the bulk fluid content measured in the host rock) is sufficient to stabilize the mineral assemblage in the mylonite. This suggests that cosesimic grain size reduction resulted in fluid redistribution into the fractured domains and not necessarily in fluid infiltration. Recent experiments suggest that very small amount of water (tens of ppm) are effective in facilitating mineral reactions if sufficient porosity in present. Coseismic fracturing and creep cavitation in the mylonitized pseudotachylytes en- hance the porosity of the shear zone and result in nucleation of new phases in dilatant sites. This process keeps the grain size of the polymineralic aggregate in the grain-size sensitive creep field, thereby stabilizing strain localiza- tion in the mylonitized pseudotachylytes. This study highlights that pseudotachylytes caused by brittle faulting can be precursors of viscous, weak shear zones in the dry lower crust, indicating lower crustal earthquakes as agents of rheological change from strong, brittle lower crust, to strong lower crust with embedded fine grained, weak viscous shear zones

The Nusfjord exhumed earthquake source (Lofoten, Norway): deep crustal seismicity driven by bending of the lower plate during continental collision

The origin of earthquakes in the lower crust at depth of 20-40 km, where dominantly ductile deformation is expected, is highly debated. Exhumed networks of lower crustal coeval pseudotachylytes (quenched frictional melt produced during seismic slip) and mylonites (produced during the post- and interseismic viscous creep) provide a snapshot of the earthquake cycle at anomalously deep conditions in the crust. Such natural laboratories offer the opportunity to investigate the origin and the tectonic setting of lower crustal earthquakes.The Nusfjord East shear zone network (Lofoten, northern Norway) represents an exhumed lower crustal earthquake source, where mutually overprinting mylonites and pseudotachylytes record the interplay between coseismic slip and viscous creep (Menegon et al., 2017; Campbell and Menegon, 2019). The network is well exposed over an area of 4 km2 and consists of three main intersecting sets of ductile shear zones ranging in width from 1 cm to 1 m, which commonly nucleate on former pseudotachylyte veins. Mutual crosscutting relationships indicate that the three sets were active at the same time. Amphibole-plagioclase geothermobarometry yields consistent P-T estimates in all three sets (700-750 °C, 0.7-0.8 GPa). The shear zones separate relatively undeformed blocks of anorthosite that contain pristine pseudotachylyte fault veins. These pseudotachylytes link adjacent or intersecting shear zones, and are interpreted as fossil seismogenic faults representing earthquake nucleation as a transient consequence of ongoing, localised aseismic creep along the shear zones (Campbell et al., under review).The coeval activity of the three shear zone sets is consistent with a local extensional setting, with a bulk vertical shortening and a horizontal NNW-SSE extension. This extension direction is subparallel to the convergence direction between Baltica and Laurentia during the Caledonian Orogeny, and with the dominant direction of nappe thrusting in the Scandinavian Caledonides. 40Ar‐39Ar dating of localized upper amphibolite facies shear zones in the Nusfjord area with similar orientation to the Nusfjord East network yielded an age range of 433–413 Ma (Fournier et al., 2014; Steltenpohl et al., 2003), which indicates an origin during the collisional (Scandian) stage of the Caledonian Orogeny.We propose that the Nusfjord East brittle-viscous extensional shear zone network represents the rheological response of the lower crust to the bending of the lower plate during continental collision. (Micro)seismicity in the lower crust in collisional orogens is commonly localized in the lower plate and has extensional focal mechanisms. This has been tentatively correlated with slab rollback and bending of the lower plate (Singer et al., 2014). We interpret the Nusfjord East shear zone network as the geological record of this type of lower crustal seismicity

Dynamic evolution of porosity in lower-crustal faults during the earthquake cycle

Earthquake-induced fracturing of the dry and strong lower crust can transiently increase permeability for fluids to flow and trigger metamorphic and rheological transformations. However, little is known about the porosity that facilitates these transformations. We analyzed microstructures that have recorded the mechanisms generating porosity in the lower crust from a pristine pseudotachylyte (solidified earthquake-derived frictional melt) and a mylonitized pseudotachylyte from Lofoten, Norway to understand the evolution of fluid pathways from the coseismic to the post- and interseismic stages of the earthquake cycle. Porosity is dispersed and poorly interconnected within the pseudotachylyte vein (0.14 vol%), with a noticeably increased amount along garnet grain boundaries (0.25–0.41 vol%). This porosity formed due to a net negative volume change at the grain boundary when garnet overgrows the pseudotachylyte matrix. Efficient healing of the damage zone by fluid-assisted growth of feldspar neoblasts resulted in the preservation of only a few but relatively large interconnected pores along coseismic fractures (0.03 vol% porosity). In contrast, porosity in the mylonitized pseudotachylyte is dramatically reduced (0.02 vol% overall), because of the efficient precipitation of phases (amphibole, biotite and feldspars) into transient pores during grain-size sensitive creep. Porosity reduction on the order of &gt;85% may be a contributing factor in shear zone hardening, potentially leading to the development of new pseudotachylytes overprinting the mylonites. Our results show that earthquake-induced rheological weakening of the lower crust is intermittent and occurs when a fluid can infiltrate a transiently permeable shear zone, thereby facilitating diffusive mass transfer and creep

The earthquake cycle in the dry lower continental crust: insights from two deeply exhumed terranes (Musgrave Ranges, Australia and Lofoten, Norway)

This paper discusses the results of field-based geological investigations of exhumed rocks exposed in the Musgrave Ranges (Central Australia) and in Nusfjord (Lofoten, Norway) that preserve evidence for lower continental crustal earthquakes with focal depths of approximately 25–40 km. These studies have established that deformation of the dry lower continental crust is characterized by a cyclic interplay between viscous creep (mylonitization) and brittle, seismic slip associated with the formation of pseudotachylytes (a solidified melt produced during seismic slip along a fault in silicate rocks). Seismic slip triggers rheological weakening and a transition to viscous creep, which may be already active during the immediate post-seismic deformation along faults initially characterized by frictional melting and wall-rock damage. The cyclical interplay between seismic slip and viscous creep implies transient oscillations in stress and strain rate, which are preserved in the shear zone microstructure. In both localities, the spatial distribution of pseudotachylytes is consistent with a local (deep) source for the transient high stresses required to generate earthquakes in the lower crust. This deep source is the result of localized stress amplification in dry and strong materials generated at the contacts with ductile shear zones, producing multiple generations of pseudotachylyte over geological time. This implies that both the short- and the long-term rheological evolution of the dry lower crust typical of continental interiors is controlled by earthquake cycle deformation

Metabolic reprogramming identifies the most aggressive lesions at early phases of hepatic carcinogenesis

Metabolic changes are associated with cancer, but whether they are just bystander effects of deregulated oncogenic signaling pathways or characterize early phases of tumorigenesis remains unclear. Here we show in a rat model of hepatocarcinogenesis that early preneoplastic foci and nodules that progress towards hepatocellular carcinoma (HCC) are characterized both by inhibition of oxidative phosphorylation (OXPHOS) and by enhanced glucose utilization to fuel the pentose phosphate pathway (PPP). These changes respectively require increased expression of the mitochondrial chaperone TRAP1 and of the transcription factor NRF2 that induces the expression of the rate-limiting PPP enzyme glucose-6-phosphate dehydrogenase (G6PD), following miR-1 inhibition. Such metabolic rewiring exclusively identifies a subset of aggressive cytokeratin-19 positive preneoplastic hepatocytes and not slowly growing lesions. No such metabolic changes were observed during non-neoplastic liver regeneration occurring after two/third partial hepatectomy. TRAP1 silencing inhibited the colony forming ability of HCC cells while NRF2 silencing decreased G6PD expression and concomitantly increased miR-1; conversely, transfection with miR-1 mimic abolished G6PD expression. Finally, in human HCC patients increased G6PD expression levels correlates with grading, metastasis and poor prognosis. Our results demonstrate that the metabolic deregulation orchestrated by TRAP1 and NRF2 is an early event restricted to the more aggressive preneoplastic lesions

Dynamic Evolution of Porosity in Lower-Crustal Faults During the Earthquake Cycle

Earthquake-induced fracturing of the dry and strong lower crust can transiently increase permeability for fluids to flow and trigger metamorphic and rheological transformations. However, little is known about the porosity that facilitates these transformations. We analyzed microstructures that have recorded the mechanisms generating porosity in the lower crust from a pristine pseudotachylyte (solidified earthquake-derived frictional melt) and a mylonitized pseudotachylyte from Lofoten, Norway to understand the evolution of fluid pathways from the coseismic to the post- and interseismic stages of the earthquake cycle. Porosity is dispersed and poorly interconnected within the pseudotachylyte vein (0.14 vol%), with a noticeably increased amount along garnet grain boundaries (0.25–0.41 vol%). This porosity formed due to a net negative volume change at the grain boundary when garnet overgrows the pseudotachylyte matrix. Efficient healing of the damage zone by fluid-assisted growth of feldspar neoblasts resulted in the preservation of only a few but relatively large interconnected pores along coseismic fractures (0.03 vol% porosity). In contrast, porosity in the mylonitized pseudotachylyte is dramatically reduced (0.02 vol% overall), because of the efficient precipitation of phases (amphibole, biotite and feldspars) into transient pores during grain-size sensitive creep. Porosity reduction on the order of >85% may be a contributing factor in shear zone hardening, potentially leading to the development of new pseudotachylytes overprinting the mylonites. Our results show that earthquake-induced rheological weakening of the lower crust is intermittent and occurs when a fluid can infiltrate a transiently permeable shear zone, thereby facilitating diffusive mass transfer and creep

A Rapid and Accurate MinION-Based Workflow for Tracking Species Biodiversity in the Field

Genetic markers (DNA barcodes) are often used to support and confirm species identification. Barcode sequences can be generated in the field using portable systems based on the Oxford Nanopore Technologies (ONT) MinION sequencer. However, to achieve a broader application, current proof-of-principle workflows for on-site barcoding analysis must be standardized to ensure a reliable and robust performance under suboptimal field conditions without increasing costs. Here, we demonstrate the implementation of a new on-site workflow for DNA extraction, PCR-based barcoding, and the generation of consensus sequences. The portable laboratory features inexpensive instruments that can be carried as hand luggage and uses standard molecular biology protocols and reagents that tolerate adverse environmental conditions. Barcodes are sequenced using MinION technology and analyzed with ONTrack, an original de novo assembly pipeline that requires as few as 1000 reads per sample. ONTrack-derived consensus barcodes have a high accuracy, ranging from 99.8 to 100%, despite the presence of homopolymer runs. The ONTrack pipeline has a user-friendly interface and returns consensus sequences in minutes. The remarkable accuracy and low computational demand of the ONTrack pipeline, together with the inexpensive equipment and simple protocols, make the proposed workflow particularly suitable for tracking species under field conditions