Patients\u27 Acceptance of Smartphone Health Technology for Chronic Disease Management: A Theoretical Model and Empirical Test

Les quatre textes ont en commun de présenter certaines évolutions récentes de l’histoire politique en Allemagne. Ils prennent tous position face à trois tournants historiographiques. La notion d’histoire culturelle du politique peut servir d’emblème au premier de ces tournants : le politique est envisagé non plus comme une succession d’événements ni comme le fruit de déterminations structurelles dont il serait la superstructure ou l’écume, mais comme l’expression de valeurs et de procédures o..

Patient Demographics in Acute Care Surgery at the Ruijin Hospital in Shanghai

Acute Care Surgery is a discipline that includes trauma care, surgical critical care, and emergency surgery. It is organized in different models and provides mainly operative and nonoperative care. The aim of this study was to provide a demographic analysis of the care of surgical patients at the Emergency Department (ED) in a large teaching hospital in Shanghai, where general surgeons and orthopedic surgeons take care for most of all acute surgery. A bilingual questionnaire was developed to collect data for patients referred to the general or orthopedic surgeon in the ED (June–September 2008). Data about the gender, age, diagnosis, diagnostic tools, treatments, and outcomes were collected. A total of 255 questionnaires were collected; the most common diagnoses of patients were infections of abdominal organs and fractures. Complementary diagnostics like X-ray (59%), blood tests (36%), and ultrasound (17%) were frequently used. More than half of the patients were discharged afterwards most of them with followup. This study gives a first overview of acute care surgery of the emergency patients of the ED in a large Chinese metropolitan hospital

Enhancing the solubility and transdermal delivery of drugs using ionic liquid-in-oil microemulsions

The development of hydrophobic drug and protein delivery carriers remains a challenge. To synthesize L-(-)-carnitine-based ionic liquid (IL), this study applies the density functional theory to investigate the hydrogen bonds and van der Waals force that govern L-(-)-carnitine-based IL formation. An ionic liquid-in-oil microemulsion (IL/O ME) is then developed to facilitate the transdermal delivery of proteins and increase the solubility of drugs. IL/O ME is prepared using isopropyl myristate (IPM), Tween 80/Span 20, and L-(-)-carnitine-based IL. The skin permeation studies conducted using mouse skin show that the insulin permeation percentage of the developed IL/O ME is 3.55 folds higher than that of phosphate-buffered saline and 2.91 folds better than that of a hydrophilic L-(-)-carnitine-based IL. In addition, the solubility of two drug molecules, that is, rosiglitazone and bezafibrate, in IL/O ME is at least 49.28 folds higher than their solubility in water or IPM. Therefore, IL/O ME can significantly improve the solubility of drugs and increase the permeability of proteins (e.g., insulin), thus demonstrating a promising potential as a delivery carrier.National Research Foundation (NRF)Singapore Advanced Research & Education Network (SingAREN)This work was supported by the National Natural Science Foundation of China (21703218), the Shenzhen Science and Technology Innovation Committee (JCYJ20180507183907224 and KQTD20170809110344233), the Economic, Trade, and Information Commission of Shenzhen Municipality through the Graphene Manufacture Innovation Center (201901161514), the Guangdong Province Covid-19 Pandemic Control Research Fund (2020KZDZX1220), and the China Scholarship Council (202006120444). This work was partially supported by the Singapore Academic Research Fund (RT12/19) and the Singapore National Research Foundation Investigatorship (NRF-NRFI2018-03)

De Novo Design of Peptidic Positive Allosteric Modulators Targeting TRPV1 with Analgesic Effects.

Transient receptor potential vanilloid 1 (TRPV1) ion channel is a nociceptor critically involved in pain sensation. Direct blockade of TRPV1 exhibits significant analgesic effects but also incurs severe side effects such as hyperthermia, causing failures of TRPV1 inhibitors in clinical trials. In order to selectively target TRPV1 channels that are actively involved in pain-sensing, peptidic positive allosteric modulators (PAMs) based on the high-resolution structure of the TRPV1 intracellular ankyrin-repeat like domain are de novo designed. The hotspot centric approach is optimized for protein design; its usage in Rosetta increases the success rate in protein binder design. It is demonstrated experimentally, with a combination of fluorescence resonance energy transfer (FRET) imaging, surface plasmon resonance, and patch-clamp recording, that the designed PAMs bind to TRPV1 with nanomolar affinity and allosterically enhance its response to ligand activation as it is designed. It is further demonstrated that the designed PAM exhibits long-lasting in vivo analgesic effects in rats without changing their body temperature, suggesting that they have potentials for developing into novel analgesics

27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells

Purified microtubules have been shown to align along the static magnetic field (SMF) in vitro because of their diamagnetic anisotropy. However, whether mitotic spindle in cells can be aligned by magnetic field has not been experimentally proved. In particular, the biological effects of SMF of above 20 T (Tesla) have never been reported. Here we found that in both CNE-2Z and RPE1 human cells spindle orients in 27 T SMF. The direction of spindle alignment depended on the extent to which chromosomes were aligned to form a planar metaphase plate. Our results show that the magnetic torque acts on both microtubules and chromosomes, and the preferred direction of spindle alignment relative to the field depends more on chromosome alignment than microtubules. In addition, spindle morphology was also perturbed by 27 T SMF. This is the first reported study that investigated the cellular responses to ultra-high magnetic field of above 20 T. Our study not only found that ultra-high magnetic field can change the orientation and morphology of mitotic spindles, but also provided a tool to probe the role of spindle orientation and perturbation in developmental and cancer biology. DOI: http://dx.doi.org/10.7554/eLife.22911.00

Molecular mechanisms underlying menthol binding and activation of TRPM8 ion channel

Menthol in mints elicits a coolness sensation by selective activation of TRPM8 ion channel. Here authors dock menthol to TRPM8 and systematically validate their menthol binding models with thermodynamic mutant cycle analysis in functional tests, and shed light on TRPM8 activation by menthol at the atomic level