Huge myxoid chondrosarcoma expanded into the thoracic cavity with spinal involvement

En bloc resection is the treatment of choice of myxoid chondrosarcoma. These tumors can produce huge masses. Anatomical constraints limit the possibility to perform en bloc resection in the spine. A very huge myxoid chondrosarcoma (14.2 × 10.8 × 11.4 cm) arising from T2 to T5 and invading the whole higher left pleural cavity was observed. Surgical planning according to WBB staging system was performed. The tumor was successfully submitted to en bloc resection achieving a tumor-free margin as demonstrated by the pathologist's report. A careful planning and a multidisciplinary collaboration make possible to perform en bloc resection even in apparently impossible cases

Hemodialysis catheter-related infection caused by Pannonibacter phragmitetus: a rare case report in China

Pannonibacter phragmitetus (P. phragmitetus) is rarely related with human disease. We reported a case of catheter-related infection caused by P. phragmitetus in a 68-year-old woman on hemodialysis. The patient developed recurrent fever during hemodialysis and blood cultures were positive for P. phragmitetus. The patient’s body temperature returned to normal after intravenous cefoperazone/sulbactam treatment, and the hemodialysis catheter was locked with gentamicin and urokinase. The potential anti-infective treatment against P. phragmitetus was discussed

Evaluation of Anti-tumor and Chemoresistance-lowering Effects of Pectolinarigenin from Cirsium japonicum Fisch ex DC in Breast Cancer

Purpose: To investigate the antitumor and chemoresistance-lowering effects of pectolinarigenin on breast cancer cells.Methods: Pectolinarigenin was purified by a combination of silica gel and Sephadex LH-20 column chromatography from ethanol extracts of the aerial parts of C. japonicum DC. Breast cancer selfrenewal properties were tested by colony formation and tumor sphere formation assays. Thereafter, real-time polymerase chain reaction (PCR) was used to detect breast cancer stem cell markers. Furthermore, the effect of pectolinarigenin on breast cancer cell was evaluated by chemoresistance using 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Finally, tumor formation in nude mice was used to test the effect of pectolinarigenin on tumorigenicity of breast cancer cells in vivo.Results: The results showed that pectolinarigenin, extracted from Cirsium japonicum Fisch. ex DC., inhibited tumor cell self-renewal in MCF-7 breast cancer cells. Pectolinarigenin (25 μM) caused significant inhibition of colony formation (61.23 %, p < 0.001) and tumor sphere formation (59.49 %, p < 0.01) in MCF-7. The inhibitory effects were associated with changes in breast cancer stem cell markers. Treatment of breast cancer cells with pectolinarigenin reduced the chemoresistance of the cells to doxorubicin. At the same time, mRNA expression of chemoresistance genes (ATP binding cassette subfamily G member 2, ABCG2 and ATP binding cassette subfamily B member 1, MDR1) was repressed by pectolinarigenin. The inhibition efficiency of MDR1 and ABCG2 by 10 μM pectolinarigenin treatment was about 59.29 (p < 0.01) and 46.48 % (p < 0.01), respectively. Furthermore, pectolinarigenin reduced tumor mass in nude mice xenograft model.Conclusion: Pectolinarigenin inhibits breast cancer stem cell-like properties and lowers the chemoresistance of the cancer cells to chemotherapy. The results provide an insight into the mechanism of the anti-breast tumor effects and an experimental basis for the use of pectolinarigenin to enhance treatment of patients with breast cancer.Keywords: Pectolinarigenin, Cancer stem cells, Breast cancer, Chemoresistance, Cirsium japonicum Fisch. ex D

Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report

<p>Abstract</p> <p>Background</p> <p>Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants.</p> <p>Case presentation</p> <p>In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.</p> <p>Conclusion</p> <p>The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.</p

Self-Lubricating Polytetrafluoroethylene/Polyimide Blends Reinforced with Zinc Oxide Nanoparticles

ZnO nanoparticle reinforced polytetrafluoroethylene/polyimide (PTFE/PI) nanocomposites were prepared and their corresponding tribological and mechanical properties were studied in this work. The influences of ZnO loading, sliding load, and velocity on the tribological properties of ZnO/PTFE/PI nanocomposites were systematically investigated. Results reveal that nanocomposites reinforced with 3 wt% ZnO exhibit the optimal tribological and mechanical properties. Specifically, the wear loss decreased by 20% after incorporating 3 wt% ZnO compared to unfilled PTFE/PI. Meanwhile, the impact strength, tensile strength, and elongation-at-break of 3 wt% ZnO/PTFE/PI nanocomposite are enhanced by 85, 5, and 10% compared to pure PTFE/PI blend. Microstructure investigation reveals that ZnO nanoparticles facilitate the formation of continuous, uniform, and smooth transfer film and thus reduce the adhesive wear of PTFE/PI

Single crystal of a one-dimensional metallo-covalent organic framework.

Although polymers have been studied for well over a century, there are few examples of covalently linked polymer crystals synthesised directly from solution. One-dimensional (1D) covalent polymers that are packed into a framework structure can be viewed as a 1D covalent organic framework (COF), but making a single crystal of this has been elusive. Herein, by combining labile metal coordination and dynamic covalent chemistry, we discover a strategy to synthesise single-crystal metallo-COFs under solvothermal conditions. The single-crystal structure is rigorously solved using single-crystal electron diffraction technique. The non-centrosymmetric metallo-COF allows second harmonic generation. Due to the presence of syntactic pendant amine groups along the polymer chains, the metallopolymer crystal can be further cross-linked into a crystalline woven network

Microscopic Examination of Polymeric Monoguanidine, Hydrochloride-Induced Cell Membrane Damage in Multidrug-Resistant Pseudomonas aeruginosa

Advances in antimicrobial activities of molecule-containing, multiple guanidinium groups against antibiotics-resistant bacteria should be noted. The synthesized polyoctamethylene monoguanidine hydrochloride (POGH), carrying cationic amphiphilic moieties, display excellent activity against multidrug-resistant Pseudomonas aeruginosa (MDR-PA) and other antibiotics-resistant bacteria. The membrane damage effects of POGH on MDR-PA were clarified using beta-lactamase activity assay, confocal fluorescence microscopy, scanning electron microscopy, and transmission electron microscopy. The results showed that POGH disrupted both the outer and inner membranes and the intracellular structure of MDR-PA to different extents depending on the dose. All concentrations of POGH within 3–23 μg/mL increased the outer membrane permeability, which facilitated the release of beta-lactamase across the inner membrane. A median dose (10 μg/mL) of POGH led to the separation of the inner and outer membrane, an increase in the membrane gap, and outer membrane structure damage with still maintained overall cytoskeletal structures. The application of a 30 μg/mL dose of POGH led to the collapse of the outer membrane, cellular wrinkling, and shrinkage, and the formation of local membrane holes. The disruption of the outer and inner membranes and the formation of the local membrane holes by a relative high dose were probably the main bactericidal mechanism of POGH. The microscopic evidence explained the strong outer-membrane permeation ability of guanidine-based antimicrobial polymers, which could be considered for the molecular design of novel guanidine-based polymers, as well as the damaged membrane structure and intracellular structure of MDR-PA

Experimental Research on the Rotating Stall of a Pump Turbine in Pump Mode

The rotating stall is an unstable flow phenomenon of pump turbines in pump mode, which is of increasing concern to scientists and engineers working on pump turbines. However, at present, various studies are carried out based on CFD (computational fluid dynamics) simulation, while directly measured data and experimental research on flow fields are seldom reported. By utilizing PIV (particle image velocimetry) measuring equipment, the flow field within the guide vane zone of a low specific speed pump turbine in pump mode was measured. By measuring and analyzing the transient flow field, the evolutionary process of the rotating stall within the guide vane passages was determined. We found that for all three tested guide vane openings, regardless of whether the positive slope appeared or not, a pre-stall operating point was found for each opening in the process of decreasing the flow rate. The analysis of the loss within the flow field indicated that the dissipation-induced loss increased greatly after the rotating stall appeared. The pump performance curves at the three guide vane openings showed an inflection at the pre-stall point. When the flow rate is larger than that of the pre-stall point, the head of the pump turbine dramatically increases as the flow rate decreases. However, when the flow rate is smaller than the pre-stall point, such increases noticeably slows down. The research results showed that whether the positive slope on the pump performance curve occurred or not, instability caused by the rotating stall should be of great concern

Research on the side converter system of wind power grid based on fractional LCL filter

Abstract The power electronic converters and grid‐connected filters were important components of the permanent magnet direct drive wind power generation system whose performance directly determines the quality of wind power generation. In past modeling, analysis, and control studies, capacitive and inductive components were often treated as integral‐order components. However, the inductance and capacitance components in the actual permanent magnet direct drive wind generator were fractional‐order components, and their electrical characteristics will change with the change of order, which had an important impact on the dynamic and static characteristics of the system. In this paper, the mathematical model of the fractional‐order LCL (FOLCL) filter was derived. Through simulation, it could be seen that the FOLCL filter can avoid resonance fundamentally. At the same time, the fractional‐order PI (FOPI) controller was introduced into the machine‐side current converter, and the parameters of the FOPI controller of the outer speed loop and the inner current loop were adjusted by using the time‐domain optimization method. The results showed that the efficiency of the FOPI controller was significantly better than that of the integer‐order PI controller in realizing maximum wind energy capture. It provides theoretical support and practical application value for the stable operation of the wind power generation system