Detection of multi-tomato leaf diseases (late blight, target and bacterial spots) in different stages by using a spectral-based sensor.

Several diseases have threatened tomato production in Florida, resulting in large losses, especially in fresh markets. In this study, a high-resolution portable spectral sensor was used to investigate the feasibility of detecting multi-diseased tomato leaves in different stages, including early or asymptomatic stages. One healthy leaf and three diseased tomato leaves (late blight, target and bacterial spots) were defined into four stages (healthy, asymptomatic, early stage and late stage) and collected from a field. Fifty-seven spectral vegetation indices (SVIs) were calculated in accordance with methods published in previous studies and established in this study. Principal component analysis was conducted to evaluate SVIs. Results revealed six principal components (PCs) whose eigenvalues were greater than 1. SVIs with weight coefficients ranking from 1 to 30 in each selected PC were applied to a K-nearest neighbour for classification. Amongst the examined leaves, the healthy ones had the highest accuracy (100%) and the lowest error rate (0) because of their uniform tissues. Late stage leaves could be distinguished more easily than the two other disease categories caused by similar symptoms on the multi-diseased leaves. Further work may incorporate the proposed technique into an image system that can be operated to monitor multi-diseased tomato plants in fields

Detection of multi-tomato leaf diseases (\u3ci\u3elate blight, target and bacterial spots\u3c/i\u3e) in different stages by using a spectral-based sensor

Several diseases have threatened tomato production in Florida, resulting in large losses, especially in fresh markets. In this study, a high-resolution portable spectral sensor was used to investigate the feasibility of detecting multi-diseased tomato leaves in different stages, including early or asymptomatic stages. One healthy leaf and three diseased tomato leaves (late blight, target and bacterial spots) were defined into four stages (healthy, asymptomatic, early stage and late stage) and collected from a field. Fifty-seven spectral vegetation indices (SVIs) were calculated in accordance with methods published in previous studies and established in this study. Principal component analysis was conducted to evaluate SVIs. Results revealed six principal components (PCs) whose eigenvalues were greater than 1. SVIs with weight coefficients ranking from 1 to 30 in each selected PC were applied to a K-nearest neighbor for classification. Amongst the examined leaves, the healthy ones had the highest accuracy (100%) and the lowest error rate (0) because of their uniform tissues. Late stage leaves could be distinguished more easily than the two other disease categories caused by similar symptoms on the multi-diseased leaves. Further work may incorporate the proposed technique into an image system that can be operated to monitor multi-diseased tomato plants in fields

S3: Social-network Simulation System with Large Language Model-Empowered Agents

Social network simulation plays a crucial role in addressing various challenges within social science. It offers extensive applications such as state prediction, phenomena explanation, and policy-making support, among others. In this work, we harness the formidable human-like capabilities exhibited by large language models (LLMs) in sensing, reasoning, and behaving, and utilize these qualities to construct the S$^3$ system (short for $\textbf{S}$ocial network $\textbf{S}$imulation $\textbf{S}$ystem). Adhering to the widely employed agent-based simulation paradigm, we employ prompt engineering and prompt tuning techniques to ensure that the agent's behavior closely emulates that of a genuine human within the social network. Specifically, we simulate three pivotal aspects: emotion, attitude, and interaction behaviors. By endowing the agent in the system with the ability to perceive the informational environment and emulate human actions, we observe the emergence of population-level phenomena, including the propagation of information, attitudes, and emotions. We conduct an evaluation encompassing two levels of simulation, employing real-world social network data. Encouragingly, the results demonstrate promising accuracy. This work represents an initial step in the realm of social network simulation empowered by LLM-based agents. We anticipate that our endeavors will serve as a source of inspiration for the development of simulation systems within, but not limited to, social science

Children neuropsychological and behavioral scale-revision 2016 in the early detection of autism spectrum disorder

BackgroundThe Children Neuropsychological and Behavioral Scale-Revision 2016 (CNBS-R2016) is a widely used developmental assessment tool for children aged 0–6 years in China. The communication warning behavior subscale of CNBS-R2016 is used to assess the symptoms of autism spectrum disorder (ASD), and its value of >30 points indicates ASD based on CNBS-R2016. However, we observed that children with relatively lower values were also diagnosed with ASD later on in clinical practice. Thus, this study aimed to identify the suitable cutoff value for ASD screening recommended by the communication warning behavior of CNBS-R2016.Materials and methodsA total of 90 typically developing (TD) children and 316 children with developmental disorders such as ASD, developmental language disorder (DLD), and global developmental delay (GDD; 130 in the ASD group, 100 in the DLD group, and 86 in the GDD group) were enrolled in this study. All subjects were evaluated based on the CNBS-R2016. The newly recommended cutoff value of communication warning behavior for screening ASD was analyzed with receiver operating curves.ResultsChildren in the ASD group presented with lower developmental levels than TD, DLD, and GDD groups in overall developmental quotient assessed by CNBS-R2016. We compared the consistency between the scores of communication warning behavior subscale and Autism Behavior Checklist (ABC), Childhood Autism Rating Scale (CARS), Autism Diagnostic Observation Schedule, second edition (ADOS-2), and clinical diagnosis for the classification of ASD at a value of 30 based on the previously and newly recommended cutoff value of 12 by the CNBS-R2016. The Kappa values between the communication warning behavior and ABC, CARS, ADOS-2, and clinical diagnosis were 0.494, 0.476, 0.137, and 0.529, respectively, with an agreement rate of 76.90%, 76.26%, 52.03%, and 82.27%, respectively, when the cutoff point was 30. The corresponding Kappa values were 0.891, 0.816, 0.613, and 0.844, respectively, and the corresponding agreement rate was 94.62%, 90.82%, 90.54%, and 93.10%, respectively, when the cutoff point was 12.ConclusionThe communication warning behavior subscale of CNBS-R2016 is important for screening ASD. When the communication warning behavior score is 12 points or greater, considerable attention and further comprehensive diagnostic evaluation for ASD are required to achieve the early detection and diagnosis of ASD in children

Mesenchymal–endothelial transition contributes to cardiac neovascularization

Endothelial cells contribute to a subset of cardiac fibroblasts by undergoing endothelial-to-mesenchymal-transition, but whether cardiac fibroblasts can adopt an endothelial cell fate and directly contribute to neovascularization after cardiac injury is not known. Here, using genetic fate map techniques, we demonstrate that cardiac fibroblasts rapidly adopt an endothelial cell like phenotype after acute ischemic cardiac injury. Fibroblast derived endothelial cells exhibit anatomical and functional characteristics of native endothelial cells. We show that the transcription factor p53 regulates such a switch in cardiac fibroblast fate. Loss of p53 in cardiac fibroblasts severely decreases the formation of fibroblast derived endothelial cells, reduces post infarct vascular density and worsens cardiac function. Conversely, stimulation of the p53 pathway in cardiac fibroblasts augments mesenchymal to endothelial transition, enhances vascularity and improves cardiac function. These observations demonstrate that mesenchymal-to-endothelial-transition contributes to neovascularization of the injured heart and represents a potential therapeutic target for enhancing cardiac repair

An Improved Method of an Image Mosaic of a Tea Garden and Tea Tree Target Extraction

UAV may be limited by its flight height and camera resolution when aerial photography of a tea garden is carried out. The images of the tea garden contain trees and weeds whose vegetation information is similar to tea tree, which will affect tea tree extraction for further agricultural analysis. In order to obtain a high-definition large field-of-view tea garden image that contains tea tree targets, this paper (1) searches for the suture line based on the graph cut method in the image stitching technology; (2) improves the energy function to realize the image stitching of the tea garden; and (3) builds a feature vector to accurately extract tea tree vegetation information and remove unnecessary variables, such as trees and weeds. By comparing this with the manual extraction, the algorithm in this paper can effectively distinguish and eliminate most of the interference information. The IOU in a single mosaic image was more than 80% and the omissions account was 10%. The extraction results in accuracies that range from 84.91% to 93.82% at the different height levels (30 m, 60 m and 100 m height) of single images. Tea tree extraction accuracy rates in the mosaic images are 84.96% at a height of 30 m, and 79.94% at a height of 60 m

Tomato Leaf Diseases Classification Based on Leaf Images: A Comparison between Classical Machine Learning and Deep Learning Methods

Tomato production can be greatly reduced due to various diseases, such as bacterial spot, early blight, and leaf mold. Rapid recognition and timely treatment of diseases can minimize tomato production loss. Nowadays, a large number of researchers (including different institutes, laboratories, and universities) have developed and examined various traditional machine learning (ML) and deep learning (DL) algorithms for plant disease classification. However, through pass survey analysis, we found that there are no studies comparing the classification performance of ML and DL for the tomato disease classification problem. The performance and outcomes of different traditional ML and DL (a subset of ML) methods may vary depending on the datasets used and the tasks to be solved. This study generally aimed to identify the most suitable ML/DL models for the PlantVillage tomato dataset and the tomato disease classification problem. For machine learning algorithm implementation, we used different methods to extract disease features manually. In our study, we extracted a total of 52 texture features using local binary pattern (LBP) and gray level co-occurrence matrix (GLCM) methods and 105 color features using color moment and color histogram methods. Among all the feature extraction methods, the COLOR+GLCM method obtained the best result. By comparing the different methods, we found that the metrics (accuracy, precision, recall, F1 score) of the tested deep learning networks (AlexNet, VGG16, ResNet34, EfficientNet-b0, and MobileNetV2) were all better than those of the measured machine learning algorithms (support vector machine (SVM), k-nearest neighbor (kNN), and random forest (RF)). Furthermore, we found that, for our dataset and classification task, among the tested ML/DL algorithms, the ResNet34 network obtained the best results, with accuracy of 99.7%, precision of 99.6%, recall of 99.7%, and F1 score of 99.7%