Antioxidants that protect mitochondria reduce interleukin-6 and oxidative stress, improve mitochondrial function, and reduce biochemical markers of organ dysfunction in a rat model of acute sepsis

Funding This study was funded by the Medical Research Council (Grant number G0800149). Research material from this study is not available. Acknowledgement We are very grateful to Dr Robin A.J. Smith, Department of Chemistry, University of Otago, Dunedin, New Zealand, for the generous gifts of MitoE and MitoQ, without which this work would not have been possible.Peer reviewedPublisher PD

Pharmacological activation of endogenous protective pathways against oxidative stress under conditions of sepsis

Funding The study was funded entirely by institutional funds.Peer reviewedPostprin

Accounting for modern corporations: Instruction paper; Accounts of holding companies; Corporation accounting and investigations; Profits of a corporation

The views of three men whose right to be considered authorities on this subject is unquestioned are presented herein. Mr. Lybrand presents a valuable paper on the subject, The Accounts of Holding Companies; Mr. Macpherson writes on Corporation Accounting and Investigations, illustrated with a worked-out problem; Mr. Dickinson presents a paper entitled The Profits of a Corporation, and in his treatment of accounting problems includes an illustrative example

Infrared aircraft measurements of stratospheric composition over Antarctica during September 1987

The JPL Mark IV interferometer recorded high resolution, infared solar spectra from the NASA DC-8 aircraft during flights over Antarctica in September 1987. The atmospheric absorption features in these spectra were analyzed to determine the overburdens of O3, NO, NO2, HNO3, ClONO2, HCl, HF, CH4, N2O, CO, H2O and CFC-12. The spectra were obtained at latitudes which ranged between 64 degrees S and 86 degrees S, allowing the composition in the interior of the polar vortex to be compared with that at the edge. The latitude dependence observed for NO, HO2, HNO3, ClONO2, HCl and HF are summerized. The values at 30 deg S were observed on the ferry flight from New Zealand to Hawaii. The dashed lines connecting the two were interpolated across the region for which there are no measurements. The chemically perturbed region is seen to consist of a collar of high HNO3 and ClONO2 surrounding a core in which the overburdens of these and of HCl and NO2 are very low. Clear increases in the overburdens of HF and HNO3 were observed during the course of September in the vortex core. HCl and NO2 exhibited smaller, less significant increases. The overburdens of the tropospheric source gases, N2O, CH4, CF2Cl2, and H2O were observed to much smaller over Antarctica than at mid-latitudes. This, together with the fact that HF over Antarctica was more that double its mid-latitude value, suggests that downwelling has occurred

Evaluation of the Psoriasis Transcriptome across Different Studies by Gene Set Enrichment Analysis (GSEA)

Our objective was to develop a consistent molecular definition of psoriasis. There have been several published microarray studies of psoriasis, and we compared disease-related genes identified across these different studies of psoriasis with our own in order to establish a consensus.We present a psoriasis transcriptome from a group of 15 patients enrolled in a clinical study, and assessed its biological validity using a set of important pathways known to be involved in psoriasis. We also identified a key set of cytokines that are now strongly implicated in driving disease-related pathology, but which are not detected well on gene array platforms and require more sensitive methods to measure mRNA levels in skin tissues. Comparison of our transcriptome with three other published lists of psoriasis genes showed apparent inconsistencies based on the number of overlapping genes. We extended the well-established approach of Gene Set Enrichment Analysis (GSEA) to compare a new study with these other published list of differentially expressed genes (DEG) in a more comprehensive manner. We applied our method to these three published psoriasis transcriptomes and found them to be in good agreement with our study.Due to wide variability in clinical protocols, platform and sample handling, and subtle disease-related signals, intersection of published DEG lists was unable to establish consensus between studies. In order to leverage the power of multiple transcriptomes reported by several laboratories using different patients and protocols, more sophisticated methods like the extension of GSEA presented here, should be used in order to overcome the shortcomings of overlapping individual DEG approach

Medial and Lateral Entorhinal Cortex Differentially Excite Deep versus Superficial CA1 Pyramidal Neurons

Although hippocampal CA1 pyramidal neurons (PNs) were thought to comprise a uniform population, recent evidence supports two distinct sublayers along the radial axis, with deep neurons more likely to form place cells than superficial neurons. CA1 PNs also differ along the transverse axis with regard to direct inputs from entorhinal cortex (EC), with medial EC (MEC) providing spatial information to PNs toward CA2 (proximal CA1) and lateral EC (LEC) providing non-spatial information to PNs toward subiculum (distal CA1). We demonstrate that the two inputs differentially activate the radial sublayers and that this difference reverses along the transverse axis, with MEC preferentially targeting deep PNs in proximal CA1 and LEC preferentially exciting superficial PNs in distal CA1. This differential excitation reflects differences in dendritic spine numbers. Our results reveal a heterogeneity in EC-CA1 connectivity that may help explain differential roles of CA1 PNs in spatial and non-spatial learning and memory

Detection of a flow induced magnetic field eigenmode in the Riga dynamo facility

In an experiment at the Riga sodium dynamo facility, a slowly growing magnetic field eigenmode has been detected over a period of about 15 seconds. For a slightly decreased propeller rotation rate, additional measurements showed a slow decay of this mode. The measured results correspond satisfactory with numerical predictions for the growth rates and frequencies

Bench-to-bedside review : targeting antioxidants to mitochondria in sepsis

Peer reviewedPublisher PD

Women’s views about management and cause of urinary tract infection: qualitative interview study

Objectives To explore the views of women with urinary tract infection on the acceptability of different strategies for managing the infection, including delayed use of antibiotics, and the cause of infection