IL-12 and IL-4 activate a CD39-dependent intrinsic peripheral tolerance mechanism in CD8+ T cells

Immune responses to protein antigens involve CD4+ and CD8+ T cells, which follow distinct programs of differentiation. Naïve CD8 T cells rapidly develop cytotoxic T-cell (CTL) activity after T-cell receptor stimulation, and we have previously shown that this is accompanied by suppressive activity in the presence of specific cytokines, i.e. IL-12 and IL-4. Cytokine-induced CD8+ regulatory T (Treg) cells are one of several Treg-cell phenotypes and are Foxp3− IL-10+ with contact-dependent suppressive capacity. Here, we show they also express high level CD39, an ecto-nucleotidase that degrades extracellular ATP, and this contributes to their suppressive activity. CD39 expression was found to be upregulated on CD8+ T cells during peripheral tolerance induction in vivo, accompanied by release of IL-12 and IL-10. CD39 was also upregulated during respiratory tolerance induction to inhaled allergen and on tumor-infiltrating CD8+ T cells. Production of IL-10 and expression of CD39 by CD8+ T cells was independently regulated, being respectively blocked by extracellular ATP and enhanced by an A2A adenosine receptor agonist. Our results suggest that any CTL can develop suppressive activity when exposed to specific cytokines in the absence of alarmins. Thus negative feedback controls CTL expansion under regulation from both nucleotide and cytokine environment within tissues

Observations of Intrahour Variable Quasars: Scattering in our Galactic Neighbourhood

Interstellar scintillation (ISS) has been established as the cause of the random variations seen at centimetre wavelengths in many compact radio sources on timescales of a day or less. Observations of ISS can be used to probe structure both in the ionized insterstellar medium of the Galaxy, and in the extragalactic sources themselves, down to microarcsecond scales. A few quasars have been found to show large amplitude scintillations on unusually rapid, intrahour timescales. This has been shown to be due to weak scattering in very local Galactic ``screens'', within a few tens of parsec of the Sun. The short variability timescales allow detailed study of the scintillation properties in relatively short observing periods with compact interferometric arrays. The three best-studied ``intrahour variable'' quasars, PKS 0405-385, J1819+3845 and PKS 1257-326, have been instrumental in establishing ISS as the principal cause of intraday variability at centimetre wavelengths. Here we review the relevant results from observations of these three sources.Comment: 10 pages, 4 figures, to appear in Astronomical and Astrophysical Transaction

One-nucleon transfer reactions and the optical potential

We provide a summary of new developments in the area of direct reaction theory with a particular focus on one-nucleon transfer reactions. We provide a status of the methods available for describing (d,p) reactions. We discuss the effects of nonlocality in the optical potential in transfer reactions. The results of a purely phenomenological potential and the optical potential obtained from the dispersive optical model are compared; both point toward the importance of including nonlocality in transfer reactions explicitly. Given the large ambiguities associated with optical potentials, we discuss some new developments toward the quantification of this uncertainty. We conclude with some general comments and a brief account of new advances that are in the pipeline.Comment: 7 pages, 5 figures, proceedings for the 14th International Conference on Nuclear Reaction Mechanisms, Varenna, June 201

Dual-Frequency Observations of 140 Compact, Flat-Spectrum Active Galactic Nuclei for Scintillation-Induced Variability

The 4.9 GHz Micro-Arcsecond Scintillation-Induced Variability (MASIV) Survey detected a drop in Interstellar Scintillation (ISS) for sources at redshifts z > 2, indicating an apparent increase in angular diameter or a decrease in flux density of the most compact components of these sources, relative to their extended emission. This can result from intrinsic source size effects or scatter broadening in the Intergalactic Medium (IGM), in excess of the expected (1+z)^0.5 angular diameter scaling of brightness temperature limited sources due to cosmological expansion. We report here 4.9 GHz and 8.4 GHz observations and data analysis for a sample of 140 compact, flat-spectrum sources which may allow us to determine the origin of this angular diameter-redshift relation by exploiting their different wavelength dependences. In addition to using ISS as a cosmological probe, the observations provide additional insight into source morphologies and the characteristics of ISS. As in the MASIV Survey, the variability of the sources is found to be significantly correlated with line-of-sight H-alpha intensities, confirming its link with ISS. For 25 sources, time delays of about 0.15 to 3 days are observed between the scintillation patterns at both frequencies, interpreted as being caused by a shift in core positions when probed at different optical depths. Significant correlation is found between ISS amplitudes and source spectral index; in particular, a large drop in ISS amplitudes is observed at spectral indices of < -0.4 confirming that steep spectrum sources scintillate less. We detect a weakened redshift dependence of ISS at 8.4 GHz over that at 4.9 GHz, with the mean variance at 4-day timescales reduced by a factor of 1.8 in the z > 2 sources relative to the z < 2 sources, as opposed to the factor of 3 decrease observed at 4.9 GHz. This suggests scatter broadening in the IGM.Comment: 30 pages, 14 figures, accepted for publication in the Astronomical Journa

A Multi-Wavelength Study of the Jet, Lobes and Core of the Quasar PKS 2101-490

We present a detailed study of the X-ray, optical and radio emission from the jet, lobes and core of the quasar PKS 2101-490 as revealed by new Chandra, HST and ATCA images. We extract the radio to X-ray spectral energy distributions from seven regions of the 13 arcsecond jet, and model the jet X-ray emission in terms of Doppler beamed inverse Compton scattering of the cosmic microwave background (IC/CMB) for a jet in a state of equipartition between particle and magnetic field energy densities. This model implies that the jet remains highly relativistic hundreds of kpc from the nucleus, with a bulk Lorentz factor Gamma ~ 6 and magnetic field of order 30 microGauss. We detect an apparent radiative cooling break in the synchrotron spectrum of one of the jet knots, and are able to interpret this in terms of a standard one-zone continuous injection model, based on jet parameters derived from the IC/CMB model. However, we note apparent substructure in the bright optical knot in one of the HST bands. We confront the IC/CMB model with independent estimates of the jet power, and find that the IC/CMB model jet power is consistent with the independent estimates, provided that the minimum electron Lorentz factor gamma_min > 50, and the knots are significantly longer than the jet width, as implied by de-projection of the observed knot lengths.Comment: 16 pages, 10 figures, 6 table

Why Do Compact Active Galactic Nuclei at High Redshift Scintillate Less?

The fraction of compact active galactic nuclei (AGNs) that exhibit interstellar scintillation (ISS) at radio wavelengths, as well as their scintillation amplitudes, have been found to decrease significantly for sources at redshifts z > 2. This can be attributed to an increase in the angular sizes of the \muas-scale cores or a decrease in the flux densities of the compact \muas cores relative to that of the mas-scale components with increasing redshift, possibly arising from (1) the space-time curvature of an expanding Universe, (2) AGN evolution, (3) source selection biases, (4) scatter broadening in the ionized intergalactic medium (IGM) and intervening galaxies, or (5) gravitational lensing. We examine the frequency scaling of this redshift dependence of ISS to determine its origin, using data from a dual-frequency survey of ISS of 128 sources at 0 < z < 4. We present a novel method of analysis which accounts for selection effects in the source sample. We determine that the redshift dependence of ISS is partially linked to the steepening of source spectral indices ({\alpha}^8.4_4.9) with redshift, caused either by selection biases or AGN evolution, coupled with weaker ISS in the {\alpha}^8.4_4.9 < -0.4 sources. Selecting only the -0.4 < {\alpha}^8.4_4.9 < 0.4 sources, we find that the redshift dependence of ISS is still significant, but is not significantly steeper than the expected (1+z)^0.5 scaling of source angular sizes due to cosmological expansion for a brightness temperature and flux-limited sample of sources. We find no significant evidence for scatter broadening in the IGM, ruling it out as the main cause of the redshift dependence of ISS. We obtain an upper limit to IGM scatter broadening of < 110\muas at 4.9 GHz with 99% confidence for all lines of sight, and as low as < 8\muas for sight-lines to the most compact, \sim 10\muas sources.Comment: 38 pages, 13 figures, accepted for publication in The Astrophysical Journa

Continuing a Chandra Survey of Quasar Radio Jets

We are conducting an X-ray survey of flat spectrum radio quasars (FSRQs) with extended radio structures. We summarize our results from the first stage of our survey, then we present findings from its continuation. We have discovered jet X-ray emission from 12 of our first 20 Chandra targets, establishing that strong 0.5-7.0 keV emission is a common feature of FSRQ jets. The X-ray morphology is varied, but in general closely matches the radio structure until the first sharp radio bend. In the sources with optical data as well as X-ray detections we rule out simple synchrotron models for X-ray emission, suggesting these systems may instead be dominated by inverse Compton (IC) scattering. Fitting models of IC scattering of cosmic microwave background photons suggests that these jets are aligned within a few degrees of our line of sight, with bulk Lorentz factors of a few to ten and magnetic fields a bit stronger than $10^{-5}$ G. In the weeks prior to this meeting, we have discovered two new X-ray jets at $z > 1$. One (PKS B1055+201) has a dramatic, $20''$-long jet. The other (PKS B1421-490) appears unremarkable at radio frequencies, but at higher frequencies the jet is uniquely powerful: its optically-dominated, with jet/core flux ratios of 3.7 at 1 keV and 380 at 480 nm.Comment: 4 pages, 8 figures. To appear in `X-Ray and Radio Connections', ed. L.O. Sjouwerman and K.K. Dyer (published electronicly at http://www.aoc.nrao.edu/events/xraydio/). Additional material and higher resolution figures may be found at http://space.mit.edu/home/jonathan/jets