Estudos sobre a nutrição mineral do milho: II - deficiências de macronutrientes na variedade Piranão

Corn plants, Piranão variety, were grown in nutrient solution, complete and with omission of one macronutrient each time, in order to gain information on the effects of treatments on growth, symptoms of deficiency, and mineral composition of the leaves. The following symptoms of deficiency were obtained: Nitrogen -- plants underdeveloped; "V" shaped chlorosis beginning in the older leaves followed by drying and necrosis; Phosphorus - older leaves dark green with a purple hve; Potassium - plants underdeveloped; marginal chlorisis and necrosis in the older leaves; iron induced chlorosis in the younger leaves; Calcium - plants very underdeveloped due to death of apical merystem; younger leaves showing tip and marginal chlorosis and afterwards necrosis and break down of the tissue; wide whitish areas in the younger leaves; Magnesium - plants very underdeveloped; internerval chlorosis in older leaves first, proceeding to the young ones; Sulfur - absence of marked symptoms, except for a slight chlorosis in the younger leaves. Dry matter production obeyed the following decreasing order: Complete, minus S, minus P, minus K, minus Mg, minus N and minus Ca. Ears were obtained only in treatments complete and minus S. Top/root ratio decreased according to this order: minus S, complete, minus K, minus Mg, minus N, minus P and minus Ca. The macronutrient content for the treatment complete was the following respectively for lower and upper, leaves: N - 2.76 and 2.52%; P - 0.59 and 0.53%; K - 2.25 and 2.26%;Ca - 2.17 and 1.85;Mg - 0.82 and0.66;S - 0.38 and 0.32. The values of the element corresponding to each treatment were: N - 1.84 and 1.22 (that is: lower leaves in the minus N plants had 1 84% N, whereas the upper leaves in same treatment had 1.22% N; P - 0.14 and 0.17%; K - 0.29 and 0.78%; Ca - 0.29 and 0.05; Mg -0.09 and 0.05%; S- 0.31 and 0.26%.Foram induzidos sintomas de carência de macronutrientes no milho, var. Piranão, exceto no caso do enxofre. Os níveis foliares de N, P, K, Ca, Mg e S foram determinados nas plantas sujeitos à nutrição completa e às deficiências de macronutrientes

Association of fenofibrate therapy with long-term cardiovascular risk in statin-treated patients with type 2 diabetes

IMPORTANCE: Patients with type 2 diabetes are at high risk of cardiovascular disease (CVD) in part owing to hypertriglyceridemia and low high-density lipoprotein cholesterol. It is unknown whether adding triglyceride-lowering treatment to statin reduces this risk. OBJECTIVE: To determine whether fenofibrate reduces CVD risk in statin-treated patients with type 2 diabetes. DESIGN, SETTING, AND PARTICIPANTS: Posttrial follow-up of the Action to Control Cardiovascular Risk in Diabetes (ACCORD) Lipid Study between July 2009 and October 2014; 5 years of follow-up were completed for a total of 9.7 years at general community and academic outpatient research clinics in the United States and Canada. Of the original 5518 ACCORD Lipid Trial participants, 4644 surviving participants were selected based on the presence of type 2 diabetes and either prevalent CVD or CVD risk factors and high-density lipoprotein levels less than 50 mg/dL (<55 mg/dL for women and African American individuals). INTERVENTIONS: Passive follow-up of study participants previously treated with fenofibrate or masked placebo. MAIN OUTCOMES AND MEASURES: Occurrence of cardiovascular outcomes including primary composite outcome of fatal and nonfatal myocardial infarction and stroke in all participants and in prespecified subgroups. RESULTS: The 4644 follow-on study participants were broadly representative of the original ACCORD study population and included significant numbers of women (n = 1445; 31%), nonwhite individuals (n = 1094; 21%), and those with preexisting cardiovascular events (n = 1620; 35%). Only 4.3% of study participants continued treatment with fenofibrate following completion of ACCORD. High-density lipoprotein and triglyceride values rapidly equalized among participants originally randomized to fenofibrate or placebo. Over a median total postrandomization follow-up of 9.7 years, the hazard ratio (HR) for the primary study outcome among participants originally randomized to fenofibrate vs placebo (HR, 0.93; 95% CI, 0.83-1.05; P = .25) was comparable with that originally observed in ACCORD (HR, 0.92; 95% CI, 0.79-1,08; P = .32). Despite these overall neutral results, we continued to find evidence that fenofibrate therapy effectively reduced CVD in study participants with dyslipidemia, defined as triglyceride levels greater than 204 mg/dL and high-density lipoprotein cholesterol levels less than 34 mg/dL (HR, 0.73; 95% CI, 0.56-0.95). CONCLUSIONS AND RELEVANCE: Extended follow-up of ACCORD-lipid trial participants confirms the original neutral effect of fenofibrate in the overall study cohort. The continued observation of heterogeneity of treatment response by baseline lipids suggests that fenofibrate therapy may reduce CVD in patients with diabetes with hypertriglyceridemia and low high-density lipoprotein cholesterol. A definitive trial of fibrate therapy in this patient population is needed to confirm these findings. TRIAL REGISTRATION: clinicaltrials.gov Identifier: NCT00000620

Estudos sobre a nutrição mineral do milho: II. efeito de doses crescentes de N, R e K no crescimento, produção e composição mineral da variedade Piranão em condições controladas

Maize plants, Piranão cultivar, were grown in nutrient solution with 5 levels each of N, R and K till harvest. Nitrate reductase activity and putrescine level were determined in leaves of plants in the N and K series, respectively, at silking stage. Upper and lower leaves were analysed for N, R and K in the corresponding treatments at the end of the life cycle. The main conclusions were the following : 5.1 There was a linear effect of level of N in the substrate on dry matter production. 5.2. High activities of nitrate reductase suggest that under natural conditions the variety should be very responsive to N fertilization. 5.3. An asymptotic response curve was found in the treatments with increasing levels of R in the medium, as well as in the case of the K treatments. 5.4. Curves of response of roots (dry matter) showed a tendency to level of as a function of increasing levels of element at lower concentrations than the components aeral part of the plant (leaves, stems, ears). 5.5. Determination of leaf putrescine did not of prove a better indicator of the K status leaf K. 5.6. The variety under study seems to be relatively more efficient in the utilization of N for yield components; the efficiency for utilization of K, is rather low and that for R is intermediate, findings that should have a bearing on the fertilization in field conditions.O milho, var. Piranão, foi cultivado em solução nutritiva com níveis crescentes de N, R e K. Houve resposta linear à adição de N e assintótica às doses de R e de K. A determinação da atividade da reductase de nitrato se correlacionou melhor com a produção da matéria seca que a do N total nas folhas. O teor de potássio total nas folhas, por sua vez refletiu melhor o estado nutricional que a determinação de putrescina nas folhas

Atividade de nanoformulações de Melaleuca alternifolia e terpinen-4-ol em isolados de Rhodococcus equi

Rhodococcus equi é o agente etiológico da rodococose equina, importante doença respiratória de potros. Especialmente na última década, a emergência de cepas resistentes aos antimicrobianos empregados no tratamento da rodococose tem sido relatada. Nesse sentido, há a necessidade de estudos envolvendo terapias alternativas e novas tecnologias, incluindo o uso de plantas medicinais e nanotecnologia. Neste trabalho utilizou-se Melaleuca alternifolia nas seguintes formulações: óleo livre, nanocápsula, nanoemulsão e a combinação de óleo livre com nanocápsula e com nanoemulsão, além do seu composto majoritário, terpinen-4-ol, a fim de verificar a atividade antimicrobiana frente a isolados de R. equi de diferentes origens. Utilizou-se o método de microdiluição em caldo na determinação das concentrações inibitória mínima (CIM) e bactericida mínima (CBM) das diferentes formulações frente aos isolados (n=24). Verificou-se baixo potencial para atividade antibacteriana de M. alternifolia na formulação de óleo livre. Todavia, essa atividade foi potencializada quando se incorporou o óleo essencial às nanoformulações. O composto terpinen-4-ol demonstrou potencial atividade antibacteriana quando incorporado ao óleo essencial e quando utilizado isoladamente. Verificou-se que tanto M. alternifolia quanto terpinen-4-ol testados possuem atividade antimicrobiana contra isolados de R. equi, sugerindo seu emprego em estudos avaliando seu potencial para o tratamento da rodococose

Microbiota cloacal aeróbia de cracídeos cativos no Rio Grande do Sul e sua susceptibilidade a antimicrobianos Cloacal microbiota identification and evaluation of the antimicrobial resistance in captive cracids from Rio Grande do Sul, Brazil

Os cracídeos são aves silvestres que habitam as matas tropicais da América. Foram coletadas, no ano de 2007, amostras cloacais de 51 aves de dez espécies diferentes de cracídeos mantidos em cativeiros no Estado do Rio Grande do Sul. A partir dos swabs, colhidos assepticamente, foi realizado o isolamento e a caracterização bacteriana e o teste de susceptibilidade antimicrobiana dos isolados. Foram identificadas 93 cepas de bactérias. As bactérias mais frequentemente isoladas foram Escherichia coli, Staphylococcus spp. e Streptococcus spp. Todas as amostras foram negativas para o isolamento de Salmonella spp. O resultado do teste de sensibilidade mostrou que dentre as 93 cepas isoladas, todas foram sensíveis apenas ao imipinem. Adicionalmente, os menores percentuais de resistência foram observados frente ao cloranfenicol e ciprofloxacina. Os gêneros e espécies bacterianas com maior percentual de resistência a diferentes antibióticos testados foram Escherichia coli, Serratia marcescens, Staphylococcus aureus e Streptococcus spp. Com os resultados obtidos no presente trabalho, concluí-se, que a população de cracídeos estudada apresenta sua microbiota cloacal composta por vários gêneros e espécies bacterianas e que a multirresistencia pode ser um problema no futuro, uma vez que algumas cepas isoladas mostraram percentuais elevados de resistência a diferente antimicrobianos.<br>Cracids are wildlife Galliformes which inhabits the America's tropical forests. Fifty one cloacal swabs were collected from 10 different species of captive cracids from the Rio Grande do Sul State during 2007. The cloacal swab samples were submitted to bacterial isolation, identification and, subsequently; antimicrobial susceptibility testing. Ninety three bacterial isolates were obtained from the cracid population examined. The most prevalent among the isolates were Escherichia coli, and bacteria from the Staphylococcus and Streptococcus genera. All samples tested in this study were negative for Salmonella spp. The antimicrobial susceptibility tests showed that none of the 93 strains presented resistance to the antimicrobial imipinem. In addition, the lower percentages of resistance were observed against cloranfenicol and ciprofloxacine. The bacteria genus and species with the highest percentage of resistance to the different antimicrobials examined were E. coli, Serratia marcescens, Staphylococcus spp. and Streptococcus spp. In conclusion, the data presented in this article demonstrate that the cloacal microbiota of the reported cracid population is composed of several bacterial genera and species and multi-drug resistance may be a problem for the future, since some strains showed elevated percentage of resistance against several different antimicrobials

Consorciação de braquiárias com milho outonal em plantio direto sob pivô central Consortium of pasture with fall corn in no tillage under center pivot

Em regiões de clima tropical, com altas taxas de decomposição do material orgânico, uma alternativa para suprir o aporte anual de palha exigido para proporcionar a sustentabilidade do sistema plantio direto é a consorciação de forrageiras com culturas produtoras de grãos. Com o objetivo de identificar, no sistema de integração agricultura-pecuária, a melhor modalidade de consorciação de três espécies de braquiária (Brachiaria brizantha B. decumbens, e B. ruzizienses) com milho outonal (linha, entrelinha e entrelinha na época de adubação de cobertura do milho), foi conduzido o presente trabalho. Foi utilizado o delineamento em blocos ao acaso, no esquema fatorial (3x3), com quatro repetições. Foram avaliadas: massa seca de palha do milho, massa seca da palha de braquiária, massa seca total de palha, massa de 1.000 grãos e produtividade de grãos. Os resultados demonstraram que, quando o foco principal da consorciação de milho com braquiária for produtividade de grãos e o foco secundário for formação de palha, a utilização da Brachiaria ruzizienses semeada na época de adubação de cobertura do milho proporcionou maiores valores de produtividade de grãos. Todos os tratamentos produziram quantidade de palha suficiente para a manutenção da estabilidade do sistema de semeadura direta.<br>In tropical regions with high rates of organic material decomposition, an alternative to supply the annual amount of straw required to provide the no tillage system sustainability is the use of intercrop of forage plants with grain crop. The present work was conducted with the objective of identify the best intercrop modality of three species of brachiaria (Brachiaria brizantha, B. decumbens and B. ruziziensis) with fall maize (row, interrow, interrow at the moment of maize coverage fertilization) in the pasture-agriculture integration system. It was used a randomized block design in a factorial scheme (3x3) with four repetitions evaluating dry weight of maize straw, dry matter of pasture straw, total dry weight of straw, 1000 grain weight and grain yield. The results showed that when the main focus of intercropping maize with pasture for grain yield and the secondary focus is the formation of straw, the use of Brachiaria ruziziensis sown at the time of maize manuring led to the highest grain yield. All treatments produced sufficient quantity of straw to maintain the stability of no tillage