Sublethal doses of insecticide reduce thermal tolerance of a stingless bee and are not avoided in a resource choice test

Insecticides and climate change are among the multiple stressors that bees face, but little is known about their synergistic effects, especially for non-Apis bee species. In laboratory experiments, we tested whether the stingless bee Tetragonula hockingsi avoids insecticide in sucrose solutions and how T. hockingsi responds to insecticide and heat stress combined. We found that T. hockingsi neither preferred nor avoided sucrose solutions with either low (2.5 × 10−4 ng µl−1 imidacloprid or 1.0 × 10−4 ng µl−1 fipronil) or high (2.5 × 10−3 ng µl−1 imidacloprid or 1.0 × 10−3 ng µl−1 fipronil) insecticide concentrations when offered alongside sucrose without insecticide. In our combined stress experiment, the smallest dose of imidacloprid (7.5 × 10−4 ng) did not significantly affect thermal tolerance (CTmax). However, CTmax significantly reduced by 0.8°C (±0.16 SE) and by 0.5°C (±0.16 SE) when bees were fed as little as 7.5 × 10−3 ng of imidacloprid or 3.0 × 10−4 ng of fipronil, respectively, and as much as 1.5°C (±0.16 SE) and 1.2°C (±0.16 SE) when bees were fed 7.5 × 10−2 ng of imidacloprid or 3.0 × 10−2 ng of fipronil, respectively. Predictions of temperature increase, and increased insecticide use in the tropics suggest that T. hockingsi will be at increased risk of the effects of both stressors in the future

Epiphytic ant-plant obtains nitrogen from both native and invasive ant inhabitants

Ant-plants have been extensively used as model systems in the study of the evolution and ecology of mutualisms. Using a 15N isotope labeling experiment, we found that both a native ant mutualist (Philidris cordata) and an invasive ant (Pheidole megacephala) provide nitrogen to the Australian ant-plant Myrmecodia beccarii

Epiphytic ant-plant obtains nitrogen from both native and invasive ant inhabitants

Ant-plants have been extensively used as model systems in the study of the evolution and ecology of mutualisms. Using a 15N isotope labeling experiment, we found that both a native ant mutualist (Philidris cordata) and an invasive ant (Pheidole megacephala) provide nitrogen to the Australian ant-plant Myrmecodia beccarii

Epiphytic ant-plant obtains nitrogen from both native and invasive ant inhabitants

Ant-plants have been extensively used as model systems in the study of the evolution and ecology of mutualisms. Using a 15N isotope labeling experiment, we found that both a native ant mutualist (Philidris cordata) and an invasive ant (Pheidole megacephala) provide nitrogen to the Australian ant-plant Myrmecodia beccarii

Native and non-native sources of carbohydrate correlate with abundance of an invasive ant

Invasive species threaten many ecological communities and predicting which communities and sites are invasible remains a key goal of invasion ecology. Although invasive ants often reach high abundances in association with plant-based carbohydrate resources, the source and provenance of these resources are rarely investigated. We characterized carbohydrate resources across ten sites with a range of yellow crazy ant abundance in Arnhem Land, Australia and New Caledonia to determine whether yellow crazy ant (Anoplolepis gracilipes) abundance and trophic position correlate with carbohydrate availability, as well as the relative importance of native and non-native sources of carbohydrates to ant diet. In both locations, measures of yellow crazy ant abundance strongly positively correlated with carbohydrate availability, particularly honeydew production, the number of tended hemipterans, and the number of plants with tended hemipterans. In Arnhem Land, 99.6% of honeydew came from native species, whereas in New Caledonia, only 0.2% of honeydew was produced by a native hemipteran. More honeydew was available in Australia due to three common large-bodied species of Auchenorrhyncha honeydew producers (treehoppers and leafhoppers). Yellow crazy ant trophic position declined with increasing yellow crazy ant abundance indicating that in greater densities the ants are obtaining more of their diet from plant-derived resources, including honeydew and extrafloral nectar. The relationships between yellow crazy ant abundance and carbohydrate availability could not be explained by any of the key environmental variables we measured at our study sites. Our results demonstrate that the positive correlation between yellow crazy ant abundance and honeydew production is not contingent upon the provenance of the hemipterans. Native sources of carbohydrate may play an underappreciated role in greatly increasing community invasibility by ants

Invasive ants reduce abundance of small rainforest skinks

Invasive ants are among the world's most damaging invasive species, often directly or indirectly affecting native fauna. Insecticidal baits are the main method for suppressing or eradicating invasive ant populations, but their use must be considered against potential for unintended effects on native organisms. The invasive yellow crazy ant (Anoplolepis gracillipes) is widespread in the tropics, particularly on islands, where they have displaced a range of invertebrates. Effects of this ant on vertebrates, and in continental ecosystems generally, are less studied. We investigated the effects of yellow crazy ants and bait application on rainforest skinks and their invertebrate prey. We compared skink and skink prey abundance across four replicated rainforest site categories: high and low yellow crazy ant sites had both been baited but differed in yellow crazy ant activity; control sites had never had yellow crazy ants or been baited; and buffer sites had never had yellow crazy ants but had been baited. We recorded significantly lower abundance of two small skink species (Lygisaurus laevis and Saproscincus tetradactylus) in high yellow crazy ant sites compared to all other site categories. The differences persisted even after baiting reduced yellow crazy ant activity by 97.8% +/- 0.04% (mean +/- SD). A larger rainforest skink species (Carlia rubrigularis) was not negatively affected by yellow crazy ant invasion. Skink prey abundance was significantly lower in high yellow crazy ant sites compared to control sites and low yellow crazy ant sites, but not compared to buffer sites. These differences did not persist following baiting. We found no evidence that baiting negatively affects skinks or their invertebrate prey. Our data suggest that yellow crazy ants, but not the bait used to treat them, pose a direct threat to small rainforest skinks

Strategies of the invasive tropical fire ant (Solenopsis geminata) to minimize inbreeding costs

How invasive species overcome challenges associated with low genetic diversity is unclear. Invasive ant populations with low genetic diversity sometimes produce sterile diploid males, which do not contribute to colony labour or reproductive output. We investigated how inbreeding affects colony founding and potential strategies to overcome its effects in the invasive tropical fire ant, Solenopsis geminata. Our genetic analyses of field samples revealed that 13–100% of males per colony (n = 8 males per 10 colonies) were diploid, and that all newly mated queens (n = 40) were single-mated. Our laboratory experiment in which we assigned newly mated queens to nests consisting of 1, 2, 3, or 5 queens (n = 95 ± 9 replicates) revealed that pleometrosis (queens founding their nest together) and diploid male larvae execution can compensate for diploid male load. The proportion of diploid male producing (DMP) colonies was 22.4%, and DMP colonies produced fewer pupae and adult workers than non-DMP colonies. Pleometrosis significantly increased colony size. Queens executed their diploid male larvae in 43.5% of the DMP colonies, and we hypothesize that cannibalism benefits incipient colonies because queens can redirect nutrients to worker brood. Pleometrosis and cannibalism of diploid male larvae represent strategies through which invasive ants can successfully establish despite high inbreeding

Origin, behaviour, and genetics of reproductive workers in an invasive ant

Background Worker reproduction has an important influence on the social cohesion and efficiency of social insect colonies, but its role in the success of invasive ants has been neglected. We used observations of 233 captive colonies, laboratory experiments, and genetic analyses to investigate the conditions for worker reproduction in the invasive Anoplolepis gracilipes (yellow crazy ant) and its potential cost on interspecific defence. We determined the prevalence of worker production of males and whether it is triggered by queen absence; whether physogastric workers with enlarged abdomens are more likely to be reproductive, how normal workers and physogastric workers compare in their contributions to foraging and defence; and whether worker-produced males and males that could have been queen- or worker-produced differ in their size and heterozygosity. Results Sixty-six of our 233 captive colonies produced males, and in 25 of these, some males could only have been produced by workers. Colonies with more workers were more likely to produce males, especially for queenless colonies. The average number of days between the first appearance of eggs and adult males in our colonies was 54.1 ± 10.2 (mean ± SD, n = 20). In our laboratory experiment, queen removal triggered an increase in the proportion of physogastric workers. Physogastric workers were more likely to have yolky oocytes (37–54.9%) than normal workers (2–25.6%), which is an indicator of fertile or trophic egg production. Physogastric workers were less aggressive during interspecific aggression tests and foraged less than normal workers. The head width and wing length of worker-produced males were on average 4.0 and 4.3% greater respectively than those of males of undetermined source. Our microsatellite DNA analyses indicate that 5.5% of worker-produced males and 14.3% of males of undetermined source were heterozygous, which suggests the presence of diploid males and/or genetic mosaics in A. gracilipes. Conclusions Our experimental work provides crucial information on worker reproduction in A. gracilipes and its potential cost to colony defence. The ability of A. gracilipes workers to produce males in the absence of queens may also contribute to its success as an invasive species if intranidal mating can take place between virgin queens and worker-produced males

Options for reducing uncertainty in impact classification for alien species

Impact assessment is an important and cost-effective tool for assisting in the identification and prioritization of invasive alien species. With the number of alien and invasive alien species expected to increase, reliance on impact assessment tools for the identification of species that pose the greatest threats will continue to grow. Given the importance of such assessments for management and resource allocation, it is critical to understand the uncertainty involved and what effect this may have on the outcome. Using an uncertainty typology and insects as a model taxon, we identified and classified the causes and types of uncertainty when performing impact assessments on alien species. We assessed 100 alien insect species across two rounds of assessments with each species independently assessed by two assessors. Agreement between assessors was relatively low for all three impact classification components (mechanism, severity, and confidence) after the first round of assessments. For the second round, we revised guidelines and gave assessors access to each other’s assessments which improved agreement by between 20% and 30% for impact mechanism, severity, and confidence. Of the 12 potential reasons for assessment discrepancies identified a priori, 11 were found to occur. The most frequent causes (and types) of uncertainty (i.e., differences between assessment outcomes for the same species) were as follows: incomplete information searches (systematic error), unclear mechanism and/or extent of impact (subjective judgment due to a lack of knowledge), and limitations of the assessment framework (context dependence). In response to these findings, we identify actions that may reduce uncertainty in the impact assessment process, particularly for assessing speciose taxa with diverse life histories such as Insects. Evidence of environmental impact was available for most insect species, and (of the non-random original subset of species assessed) 14 of those with evidence were identified as high impact species (with either major or massive impact). Although uncertainty in risk assessment, including impact assessments, can never be eliminated, identifying, and communicating its cause and variety is a first step toward its reduction and a more reliable assessment outcome, regardless of the taxa being assessed